The invention belongs o the technical field of
biology and specifically relates to a human
aspirin resistance
gene polymorphism detection kit as well as a preparation method and application thereof. The kit is prepared from PCR premixed reaction liquid, a
positive control product and a
negative control product, wherein the PCR premixed reaction liquid, the
positive control product and the negativecontrol product are respectively used for amplifying polymorphism of 3 genes of GPIIIa, GP1BA and
PEAR1 which are related to human
aspirin resistance; the PRC premixed reaction liquid is prepared from a specificity primer sequence set, a probe set and PCR reaction liquid; the specificity primer sequence set is used for amplifying all the sites; the specificity primer set is prepared from an ordinary outer primer and an ARMs primer with fluorescent
label specificity. The kit disclosed by the invention is used for detecting the human
aspirin resistance
gene polymorphism and has the advantages of high flexibility, high specificity, convenience in operation, reliable results and the like; furthermore, detection can be finished within 1 hour, and result reading is simple and objective.