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Detection kit for detecting metabolic capability of aspirin and preparation method of detection kit

A technology for detecting kits and metabolic capabilities, applied in the field of biochemical detection, can solve problems such as high false positive rate and poor accuracy rate, improve capture efficiency and detection sensitivity, and reduce the failure or poor effect of anti-platelet aggregation therapy

Active Publication Date: 2022-02-01
湖南菲思特精准医疗科技有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

ELISA is currently still the gold standard for detecting aspirin metabolism, but the current ELISA has the problems of high false positive rate and poor accuracy

Method used

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  • Detection kit for detecting metabolic capability of aspirin and preparation method of detection kit
  • Detection kit for detecting metabolic capability of aspirin and preparation method of detection kit
  • Detection kit for detecting metabolic capability of aspirin and preparation method of detection kit

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Embodiment Construction

[0035] The detection kit for detecting the aspirin metabolism ability provided by the present invention and the preparation method thereof will be further described in detail and completely below in conjunction with the examples. The embodiments described below are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0036] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from the market unless otherwise specified.

[0037]The enzyme-linked immunosorbent assay method for the curative effect of aspirin disclosed in this embodiment is to detect the content of 11-DH-TXB2 in human urine by using enzyme-linked immunosorbent assay. The enzyme-linked immunosorbent assay in this example adopts the principle of the competition method, uses the enzyme-linked immunosorbent assay method and the enzy...

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Abstract

The invention discloses a detection kit for detecting the metabolic capability of aspirin and a preparation method of the detection kit, and the kit comprises an elisa plate coated with a monoclonal antibody of 11-dehydrothromboxane B2, a 11-dehydrothromboxane B2 competitive product, a chromogenic substrate, a stop buffer and a 11-dehydrothromboxane B2 standard solution, wherein the monoclonal antibody of 11-dehydrothromboxane B2 is a mouse monoclonal antibody, the sequence of a light chain variable region of the mouse monoclonal antibody is as shown in SEQ ID NO.2 in a sequence table, and the sequence of a heavy chain variable region of the mouse monoclonal antibody is as shown in SEQ ID NO.4 in the sequence table. The enzyme-linked immunosorbent assay kit and the method thereof have the characteristics of accuracy, high sensitivity and the like for detecting aspirin metabolism so as to judge whether a patient has aspirin drug resistance or resistance, and are not easily influenced by biochemical indexes such as creatinine and the like; and the problem of failure or poor effect of anti-platelet aggregation treatment caused by aspirin resistance of clinical patients can be greatly reduced.

Description

technical field [0001] The invention relates to a detection kit for detecting aspirin metabolism capacity and a preparation method thereof, belonging to the field of biochemical detection. Background technique [0002] 11-dehydro-thromboxane B2 (11-dehydro-thromboxaneB2, 11-DH-TXB2) is the terminal metabolite of thromboxane A2 (TXA2), which is mainly excreted through the kidneys, and is involved in hemostasis and the occurrence of cardiovascular diseases. Play an important role. Arachidonic acid generates prostaglandin H under the action of prostaglandin H synthase 1 and 2 (COX-1 and COX-2). Prostaglandin H is chemically unstable and can be converted into a variety of biologically active prostaglandins under the action of isomerase, including TXA2, prostacyclin I2, prostaglandin D2, prostaglandin E2, and prostaglandin F2α. TXA2 is mainly synthesized in platelets through the COX-1 pathway. Neonatal platelets express both COX-1 and COX-2, while mature platelets only express ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/58G01N33/543G01N33/88G01N21/78G01N21/31C07K16/44
CPCG01N33/577G01N33/581G01N33/54306G01N33/88G01N33/9486G01N21/78G01N21/31C07K16/44G01N2800/222G01N2021/786C07K2317/56
Inventor 刘丹易倩春
Owner 湖南菲思特精准医疗科技有限公司
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