The invention discloses a function and an application of a Mindin gene in treatment of atherosclerosis and belongs to the field of functions and applications of genes. In the invention, an ApoE - / - mouse and a Mindin - / - ApoE - / - mouse are employed as experimental subjects and an atherosclerosis model is obtained through high-fat diet induction. A result proves that compared with the ApoE - / - mouse, a Mindin gene defect can significantly reduce a plague area in aorta, enhance stability of aortic sinus plagues and significantly alleviate an inflammatory response, which indicate that the Mindin, in the atherosclerosis, mainly has an effect of promoting formation of a plague in the aorta and especially promoting the atherosclerosis. On the basis of the functions hereinabove of the Mindin, the Mindin, as a drug target, can be used for screening a drug for preventing, alleviating and / or treating the atherosclerosis. An inhibitor of the Mindin can be used for preparing a drug for preventing, alleviating and / or treating the atherosclerosis.