The invention relates to 7-ethyl-10-hydroxycamptothecine
crystal forms II and III, and a preparation method and application thereof. The
crystal form II uses Cu-
K alpha radiation; the X-
ray powder diffraction represented by 2theta angle has characteristic peaks when the
diffraction angle is 5.64, 10.80, 12.55, 13.48, 17.09, 17.65, 18.21, 18.79, 24.52 and 26.84 degrees; and the error range of 2theta is + / -0.2 degree. The
crystal form III uses Cu-
K alpha radiation; the X-
ray powder diffraction represented by 2theta angle has characteristic peaks when the diffraction angle is 4.58, 9.34, 10.42, 11.12, 12.30, 13.40, 14.40, 16.34, 16.86, 17.20, 17.70, 18.72, 19.32, 21.48, 22.44, 24.90, 25.40, 25.68 and 27.44 degrees; and the error range of 2theta is + / -0.2 degree. The two crystal forms of 7-ethyl-10-hydroxycamptothecine have high
solubility and favorable stability.