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Cultivation method of cordyceps sinensis sporocarps and composition and application of cordyceps sinensis sporocarps

A technology of Cordyceps sinensis seeds and entities, which is applied in the field of compositions containing Cordyceps sinensis fruiting bodies, can solve the problems of industrialization efficiency to be improved, long time for induced color change, and low biotransformation rate, so as to shorten the production time and increase the biotransformation rate Effect

Active Publication Date: 2015-03-04
SYNGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Based on the aforementioned studies, although it has been possible to cultivate the fruiting body of Cordyceps sinensis with artificial medium, the existing technology still has its shortcomings, including: low biotransformation rate, industrialization efficiency needs to be improved, and long time for induction , Low-altitude and flat cultivation conditions and stable mass production need to be overcome

Method used

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  • Cultivation method of cordyceps sinensis sporocarps and composition and application of cordyceps sinensis sporocarps
  • Cultivation method of cordyceps sinensis sporocarps and composition and application of cordyceps sinensis sporocarps
  • Cultivation method of cordyceps sinensis sporocarps and composition and application of cordyceps sinensis sporocarps

Examples

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Effect test

Embodiment 1

[0055] Screening the dominant bacterial strain of Mortierella sinensis with Cordyceps sinensis mating type gene sequence analysis technology (compatibility gene sequence analysis technology), comprises the following steps:

[0056] 1. After high-temperature disinfection of the sterile scalpel under the flame of an alcohol lamp, soak it in 75% alcohol to cool it down for later use;

[0057] 2. Take the fruiting body tissue block of Cordyceps sinensis, spray 75% alcohol on its surface for disinfection, and cut out the fruiting body tissue block of Cordyceps sinensis with the sterile scalpel described in step 1, and attach the tissue block of the fruiting body obtained by cutting Cultivate in agar medium (containing 100ppm streptomycin) at 14-20°C until hyphae are produced;

[0058] 3. Take the genomic DNA of Mortierella sinensis: collect the mycelium described in step 2 in a sterile mortar, add liquid nitrogen and grind it into powder, put it into a microcentrifuge tube, add 700...

Embodiment 2

[0064] Artificially cultivating the fruiting bodies of Cordyceps sinensis, comprising the steps of:

[0065] 1. Prepare artificial culture medium, the composition of this artificial culture medium comprises mass percent: 3-5% silkworm chrysalis extract, 1-3% peptone, 1-3% yeast extract, 2-6% glucose, 4-6% special sand, 0.2-0.6% food grade carboxymethyl cellulose, 0.05-0.2% trace elements (iron, nickel, manganese, copper, zinc, boron, molybdenum and chlorine, etc.), 1-3 % of food-grade Hantian powder or agaric gum, and water (the remaining less than 100% is water), wherein, the preparation process of the silkworm chrysalis extract is as follows: 50g of silkworm chrysalis powder is added to 1L of water and mixed uniformly, and extinguished under high temperature and high pressure. Bacteria 20 minutes, through solid-liquid separation after cooling, this liquid is silkworm chrysalis extract; Described trace element comprises (mass percentage): magnesium sulfate 0.01%, dipotassium ...

Embodiment 3

[0072] Industrial-grade mass production of fruiting bodies of Cordyceps sinensis, including the following steps:

[0073] 1. Take the mycelium of the dominant strain of Mortierella sinensis, after the action of a masher, inoculate it into a triangular flask, and carry out shaking culture, the culture temperature is 14-20°C, the speed of the incubator is 100-200rpm, and finally cultivated to Mycelium grows and covers the triangular flask and becomes the inoculum;

[0074] 2. Prepare the artificial medium, the composition of the artificial medium includes: 3-5% silkworm chrysalis extract, 1-3% peptone, 1-3% yeast extract, 2-6% glucose , 4-6% special sand, 0.2-0.6% food grade carboxymethyl cellulose, 0.05-0.2% trace elements (iron, nickel, manganese, copper, zinc, boron, molybdenum and chlorine, etc.), 1- 3% food-grade fennel powder or agaric gum, and water (the rest less than 100% is water), wherein, the preparation process of the silkworm chrysalis extract is as follows: 50g s...

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Abstract

The invention discloses a method for cultivating cordyceps sinensis sporocarps, a cordyceps sinensis sporocarp composition and an application thereof. The method for cultivating the cordyceps sinensis sporocarps comprises the following steps: screening a dominant strain, wherein the dominant strain can be used for rapidly and stably producing the cordyceps sinensis sporocarps; inoculating the dominant strain into an artificial culture medium containing a silkworm chrysalis extracting solution, peptone, a yeast extract, glucose, superfine sand, food-grade carboxymethyl cellulose, trace elements and food-grade agar powder or agarose gel; and performing cultivation in an environment with a changeable temperature and LED (Light-Emitting Diode) illumination to fulfill the aims of industrial scale and mass production of the cordyceps sinensis sporocarps. As proved by detection, the cordyceps sinensis sporocarps cultivated by using the method are higher in indicating components such as polysaccharides, cordycepic acid and adenosine than natural cordyceps sinensis sporocarps.

Description

technical field [0001] The invention relates to a cultivation technology of Cordyceps sinensis, in particular to a method capable of stably mass-producing and cultivating fruiting bodies of Cordyceps sinensis in batches artificially, and a composition containing the fruiting bodies of Cordyceps sinensis and its application. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis) is a large class of medicinal fungi, belonging to the phylum Eumycota, the subphylum Ascomycotina, the class Pyenomycetes, the family Clavicipiracese, and the genus Cordyceps. The asexual form of Cordyceps sinensis can continue to reproduce in artificial medium and artificial culture conditions. When sexual reproduction requires two homologous mycelia to undergo nuclear fusion to form a dikaryotic mycelium, and in a specific environment Induce hyphae to differentiate and form fruiting bodies. The compatibility of the two homologous mycelia in Cordyceps sinensis is determined by th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01H15/00A61K36/068
CPCA01G18/00A23L31/00A61K36/068
Inventor 陈威仁魏钰珊叶美良
Owner SYNGEN BIOTECH
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