The invention relates to a disassembly and assembly tool for optical fiber connectors. The disassembly and assembly tool comprises a base, at least one vertical column, a cross beam, a detector and a detection terminal; at least four adjusting bolts are arranged on the lower surface of the base, and a detection platform is arranged on the upper surface of the base; the tail end of the vertical column is perpendicularly connected to the edge position of the upper surface of the base; the tail end of the cross beam is arranged outside the vertical column in a sleeving mode and is connected to the vertical column in a sliding mode, the front end of the cross beam is hinged to the detector through a locating pin, and the cross beam comprises a rod body, a connecting ratchet wheel and an organ type protection cover; the detection terminal is installed right below the detector, is connected to the detector and comprises a test end, a fixed threaded sleeve, a ceramic locating rod and a test head, wherein the top end of the test end is connected to the detector, and the tail end of the test end is connected to the top end of the fixed threaded sleeve. According to the disassembly and assembly tool, the work efficiency and the work quality of the focal distance detection work of the optical fiber connectors can be improved effectively, the replacement efficiency of parts of detection equipment can be improved effectively, the service life of the parts of the detection equipment can be prolonged effectively, and the detection cost can be lowered.