The invention relates to the technical field of conductive wheels, in particular to a superconducting film preparation equipment conductive wheel and an operation method thereof.The superconducting film preparation equipment conductive wheel comprises a mounting base, a rotating mechanism is mounted on the mounting base, a fastening mechanism is mounted on the rotating mechanism, a driving mechanism is connected to the rotating mechanism, a conductive mechanism is mounted in the mounting base, and the fastening mechanism is connected to the driving mechanism. The conductive mechanism is provided with a control mechanism, and the conductive mechanism is connected with a dismounting device; through installation of the rotating mechanism and the installation base, the superconducting thin film conveying guiding effect is facilitated, through cooperation of the fastening mechanism and the driving mechanism, the rotating mechanism can be conveniently installed and disassembled, operation is convenient, follow-up maintenance is facilitated, and through cooperation of the conductive mechanism and the control mechanism, the rotating mechanism can be conveniently propped against; and under the action of the dismounting and mounting mechanism, the conductive mechanism can be dismounted and replaced conveniently, operation is convenient, maintenance is facilitated, and the stability of equipment operation is improved.