The invention relates to a preparation method for a solid-phase micro-extraction fiber bundle, and relates to preparation of the fiber bundle. The preparation method comprises the steps of (1) preparation of single SPME (solid-phase micro-extraction) fibers based on an integral material: mixing a monomer, a crosslinking agent, a pore-forming agent and an initiator to prepare a uniform solution, then injecting the uniform solution into a capillary which is 5-20cm long and of which the inner diameter is 0.2-0.8mm and the wall thickness is 0.05-0.2mm, sealing two ends of the capillary, performing polymerization, removing a section, which is 1-3cm long, of the capillary, putting the integral material fiber into a soxhlet extractor, performing extraction through a solvent, or putting the fiber into the solvent, and soaking the fiber until no impurity is detected from the liquid so as to obtain the single SPME fibers based on the integral material; (2) preparation of the solid-phase micro-extraction fiber bundle: combining 2-6 of the single SPME fibers based on the integral material on the capillary to obtain the solid-phase micro-extraction fiber bundle. The preparation method is simple, high in extraction capacity, high in extraction speed, low in cost and easy to operate.