32 results about "Transplantation procedure" patented technology

The present disclosure relates to methods to deconvolute a mixture sample of genetic material from different origins or sources. The disclosed methods can be used in various applications, including, the non-invasive determination of a fetal genome, a fetal -ome (e.g. exome). or other targeted fetal locus from cell-free nucleic acids in maternal plasma or other body fluids; the de-termination of cancer-associated mutations from cell-free nucleic acids in a body fluid sample that contains a mixture of nucleic acids from normal cells and tumor cells; and quantification of donor cell contamination using a body fluid from a transplantation recipient to monitor and / or predict the outcome of a transplantation procedure.

This invention is directed to novel oxamyl dipeptide ICE / ced-3 family inhibitor compounds. The invention is also directed to pharmaceutical compositions containing these compounds, as well as to the use of such compositions in the treatment of patients suffering inflammatory, autoimmune and neurodegenerative diseases, for the prevention of ischemic injury, and for the preservation of organs that are to undergo a transplantation procedure.

The invention provides a cultivation method capable of preventing Angelica sinensis from premature bolting. The cultivation method comprises the following steps: deeply plowing soil and fertilizing at a planting area; sowing when the temperature of soil layer of 5 cm below the ground surface is 6-8 DEG C, wherein the row spacing is 20-25 cm, opening shallow gullies with the depth of 2-3 cm, after sowing, covering wet soil, and then covering wheatgrass, wherein the seedling density per hectare is 0.45-0.75 million plants, and the plant spacing is 5-10 cm; when the height of seedling is 3-4 cm, gapping and establishing so as to keep the plant spacing and the seedling density; sowing in late October of the present year, digging and sunning when the aerial part is withered so as to obtain the Angelica sinensis. According to the cultivation method, under the precondition of ensuring the yield and quality of Angelica sinensis, the Angelica sinensis is prevented from premature bolting, the seedling and transplantation procedures of Angelica sinensis are omitted, the production period of Angelica sinensis is shortened, 25% of seedling and seedling storage land is saved, and 120-130 days of production period of Angelica sinensis are shortened.

This invention is directed to novel oxamyl depeptide ICE / ced-3 family inhibitor compounds. The invention is also directed to pharmaceutical compositions containing these compounds, as well as to the use of such compositions in the treatment of patients suffering inflammatory, autoimmune and neurodegenerative diseases, for the prevention of ischemic injury, and for the preservation of organs that are to undergo a transplantation procedure.

A method of treatment of neurodegenerative diseases using neuronal cell transplants is provided. The growth, survival and integration of the transplanted neuronal cells is enhanced by a method of culturing the neuronal cells with drugs having an affinity for immunophilins. Immunophilin binding drugs are optionally administered to the patient during transplantation and / or post-operatively. Neurotrophic factors can also be administered to the neuronal cells in vitro, or to the patient during the transplantation procedure and / or post-operatively.

This invention is directed to novel oxamyl dipeptide ICE / ced-3 family inhibitor compounds having the following structure: wherein A, B, R, R1, R1′ p and q are as defined herein. The invention is also directed to pharmaceutical compositions containing one or more of these compounds, as well as to the use of such compositions in the treatment of patients suffering inflammatory, autoimmune and neurodegenerative diseases, for the prevention of ischemic injury, and for the preservation of organs that are to undergo a transplantation procedure.

A method of inducing immune tolerance in a first mammal to antigens of a second, non-syngeneic, mammal, is disclosed. The method is utilized to minimize graft rejection and / or reduce graft-versus-host diseases in transplantation procedures and to produce hematopoietic mixed chimeras. Methods of determining the activity of tyrphostins and the optimal concentration thereof in this method are also disclosed.

The invention is directed to methods of using Flt3-Ligand in hematopoietic cell transplantation procedures using nonmyeloablative conditioning regimens. This abstract is provided for the sole purpose of enabling the reader to quickly ascertain the subject matter of the technical disclosure and is not intended to be used to interpret or limit the scope or meaning of the claims.

The invention discloses a high-yield planting method for desert date trees in saline-alkali soil. The high-yield planting method mainly includes steps of sowing and seedling raising, seedling management, saline-alkali soil field planting and field management. The step of sowing and seedling raising includes procedures of treating seedlings; sowing and raising the seedlings; raising the seedlings by cutting; raising the seedlings by layering. The step of seedling management mainly includes controlling temperature, humidity and illumination factors. The step of saline-alkali soil field planting includes field treatment and transplantation procedures. The step of field management mainly includes inter-cultivation weeding and fertilization. Sulfate compound fertilizers need to be applied instead of chlorate compound fertilizers in the step of field management. According to the technical scheme, the high-yield planting method has the advantages that the problem of wastage of large quantities of saline-alkali fields can be solved, land resources can be sufficiently utilized, the national call for environmental protection can be responded, and the high-yield planting method is high in economic benefit and social benefit.

Provided are a cell sheet and a three-dimensional structure thereof that include at least mesenchymal stem cells and myoblasts isolated from a cell culture support and that are to be applied to heart disease. Use of the cell sheet and the three-dimensional structure thereof including mesenchymal stem cells and myoblasts can notably improve cardiac functions compared with a conventional technology, i.e., use of cell sheets composed of myoblasts only or mesenchymal stem cells only. Furthermore, the cell sheet itself has high strength, and the transplantation procedure is also improved.

A method for producing a regenerative organ germ for transplantation includes using regenerative organ germ for transplantation ensuring continuity with a recipient after transplantation and facilitating transplantation procedures. A method for producing a regenerative organ germ provided with a guide for transplantation includes preparing a regenerative organ germ by closely contacting a first cell mass, which substantially consists of mesenchymal cells, and a second cell mass, which substantially consists of epithelial cells, culturing these cell masses within a support, and inserting the guide into the regenerative organ germ.

This invention is directed to novel sulfonimide (substituted)acyl dipeptidyl ICE / ced-3 family inhibitor compounds having the following structure: wherein A, B, X, R, R1, R2, n, q, and r are as defined herein. The invention is also directed to pharmaceutical compositions containing these compounds, as well as the use of such compositions in the treatment of patients suffering inflammatory, autoimmune and neurodegenerative diseases, for the prevention of ischemic injury, and for the preservation of organs that are to undergo a transplantation procedure.

A method for producing a regenerative organ germ for transplantation includes using regenerative organ germ for transplantation ensuring continuity with a recipient after transplantation and facilitating transplantation procedures. A method for producing a regenerative organ germ provided with a guide for transplantation includes preparing a regenerative organ germ by closely contacting a first cell mass, which substantially consists of mesenchymal cells, and a second cell mass, which substantially consists of epithelial cells, culturing these cell masses within a support, and inserting the guide into the regenerative organ germ.

A surface contour gauge is provided for use in surgical transplantation procedures. Surface contour gauges provide quick and efficient surface contour matches between a patient's bone defect and a prepared allograft plug.

The present invention relates to a method for preparing commercial scale quantities of human functional beta cells and to the establishment of cell lines from non-foetal pancreatic tissues. It also relates to a method of diagnosis using beta cell tumors or cells derived thereof. The method comprises sub-transplantation procedure to enrich the graft in proliferating beta cells, allowing to generate human Beta cell lines. Such lines express, produce and secrete insulin upon glucose stimulation. They have a gene expression profile that resembles to adult beta cells. In addition, the human beta cell lines are able to normalize glycemia of diabetic mice when transplanted, demonstrating their insulin secretion capabilities.

The invention provides a cultivation method capable of preventing Angelica sinensis from premature bolting. The cultivation method comprises the following steps: deeply plowing soil and fertilizing at a planting area; sowing when the temperature of soil layer of 5 cm below the ground surface is 6-8 DEG C, wherein the row spacing is 20-25 cm, opening shallow gullies with the depth of 2-3 cm, after sowing, covering wet soil, and then covering wheatgrass, wherein the seedling density per hectare is 0.45-0.75 million plants, and the plant spacing is 5-10 cm; when the height of seedling is 3-4 cm, gapping and establishing so as to keep the plant spacing and the seedling density; sowing in late October of the present year, digging and sunning when the aerial part is withered so as to obtain the Angelica sinensis. According to the cultivation method, under the precondition of ensuring the yield and quality of Angelica sinensis, the Angelica sinensis is prevented from premature bolting, the seedling and transplantation procedures of Angelica sinensis are omitted, the production period of Angelica sinensis is shortened, 25% of seedling and seedling storage land is saved, and 120-130 days of production period of Angelica sinensis are shortened.

The invention relates to methods for promoting maturation of islet cells from pre-weaned mammals for the purpose of optimizing the islets for their use as donor tissue for xenotransplantation. The method of the invention removes the pancreas from donor animals and reduces the pancreas tissue to fragments that are greater than the size of an intact islet while retaining islets in their whole, insulin-producing condition. The method of the invention also serially cultures the digested tissue in novel maturation media that enhance the glucose responsiveness of the cultured islets, and selects islets that are sufficiently glucose-responsive for use in transplantation procedures.

The present invention relates to methods for improving the viability and recovery of islets that are separated from a donor organ for subsequent transplantation. In a preferred embodiment, the islets are separated from a donor pancreas and transplanted into the liver of a diabetic patient. One or more emulsified perfluorocarbons (ePFCs) may be infused into the donor pancreas prior to islet isolation and transplantation. The ePFCs may enhance the oxygenation of islets, thereby enhancing their viability and health so they may withstand a vigorous isolation procedure such as the Edmonton Protocol. The present invention not only preserves the donor organ using ePFC, but also rescues islets and donor organs that would otherwise be damaged or destroyed during isolation and transplantation procedures. The separated islets may be injected into the portal vein of a liver where they will assist in producing insulin and regulating blood glucose levels.

This invention is directed to novel oxamyl dipeptide ICE / ced-3 family inhibitor compounds having the following structure: wherein A, B, R, R1, R1′ p and q are as defined herein. The invention is also directed to pharmaceutical compositions containing one or more of these compounds, as well as to the use of such compositions in the treatment of patients suffering inflammatory, autoimmune and neurodegenerative diseases, for the prevention of ischemic injury, and for the preservation of organs that are to undergo a transplantation procedure.

The invention provides a human embryo transplantation in-vitro simulation research platform. The platform is characterized by comprising a uterine cavity model, a pressure sensor, a micro-injection pump, a camera, a tilting table, a measurement and control box and a computer; the uterine cavity model is a nearly-closed flat triangular container which is established based on uterine cavity data ofthe human body and filled with a high-viscosity medium; the pressure sensor detects the pressure of the uterine cavity model to ensure that the uterine cavity model is the closest to the uterine cavity environment of the human body; the micro-injection pump injects a transplanted carrier into the uterine cavity model, the camera is fixed above the tilting table, a shooting lens is kept parallel tothe uterine cavity model, and a movement trajectory after the transplanted carrier enters the uterine cavity is shot. The simulation platform is established in vitro to explore the movement trajectory and deposition position of an embryo entering the uterine cavity under different transplantation procedures and different uterine positions, the technical measures of embryo transplantation are improved, the relationship between embryo movement and liquid and gas movement is observed, and the movement condition of the embryo is truly reflected.

The present invention relates to a method for preparing commercial scale quantities of human functional Betacells and to the establishment of cell lines. It also relates to a method of diagnosis using Beta cell tumors or cells derived thereof. The method comprises sub-transplantation procedure to enrich the graft in proliferating Betacells, allowing to generate human Betacell lines. Such lines express little amount of insulin and have a gene expression profile that resembles to adult Betacells. In addition, the human Betacell lines are able to normalize glycemia of diabetic mice when transplanted, demonstrating their insulin secretion capabilities.

The invention discloses a preserving fluid. The preserving fluid is prepared from one or a mixture of more than two of 5-aminolevulinic acid, L-carnosine, L-glutamic acid and D-biotin. The invention also discloses an application of the preserving fluid. Compared with the prior art, the 5-aminolevulinic acid, the L-carnosine, the L-glutamic acid and the D-biotin are added into the preserving fluid, cells, tissues and / or organs are subjected to cold preservation, the cell survival rate is high, cell apoptosis caused by cold preservation-rewarming can be effectively reduced, mitochondrial membrane potential can be stabilized, mitochondria can be prevented from excessively generating reactive oxygen free radicals, mitochondrial injury caused by cold preservation-rewarming can be effectively reduced, accordingly, ischemia reperfusion injury of donation tissues and organs in transplantation procedures can be reduced, and the preserving fluid has great significance in progress of organ transplantation preservation technologies.