The invention discloses a mobile terminal APP loading energy consumption automatic measurement method based on physical actual measurement. The method includes the steps that 1, a specified mobile terminal Web and a specified mobile terminal type are sent to a server; 2, the server transmits the mobile terminal Web into a browser running on a corresponding mobile terminal to load the mobile terminal Web, an ammeter is started to begin measurement, and the requiring time, the receiving time and the computing time of each network resource are acquired in the measurement process; 3, after the mobile terminal Web is loaded on the mobile terminal, the server processes measured data, wherein firstly, the page loading process and a timestamp in the page loading process are acquired according to the requiring time, the receiving time and the computing time of the network resources, then the obtained timestamp is synchronized with the time of the server, and finally energy consumption generated when the mobile terminal Web is loaded on the mobile terminal is computed according to the synchronized time and the measured data of the ammeter. By means of the method, measurement precision is greatly improved.