The invention discloses a mixed herbicide containing glyphosate and bispyribac-sodium, belonging to the technology field of pesticides. The herbicide takes glyphosate and bispyribac-sodium as main effective ingredients, wherein the mass ratio of glyphosate to bispyribac-sodium is (80-98):(0.1-10). The invention also discloses application of the mixed herbicide to post-emergence weeding in a paddy field. The mixed herbicide has wide weeding spectrum through mixture of weed killers, so as to improve the weeding effect; the mixed herbicide has the advantages of having quick effect, long lasting period, thorough weeding effect, low residue, low toxicity, safety, environment protection and the like and having capability of delaying pesticide resistance of the herbicide, and has prominent prevention and control effect on grassy weeds, broad leaf weeds, sedge weeds in the paddy field such as barnyard grass, leptochloa chinensis, difformed galingale herb, Eleocharis yokoscensis, Sagittaria pygmaea, Rotala indica and Marsilea quadrifolia, and therefore, the mixed herbicide is high-quality post-emergence chemical herbicide.