41 results about "Protein design" patented technology

The invention relates to the use of protein design automation (P DA) to generate computationally prescreened secondary libraries of proteins, and to methods and compositions utilizing the libraries.

The present invention discloses methods and compositions comprising a group of novel expression cassettes which provide significantly improved levels of accumulation of Coleopteran inhibitory Cry3B and Cry3B variant amino acid sequences when these are expressed in plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.

The invention relates to a method for designing proteins on the basis of polarizable force fields and pso (particle swarm optimization). The method includes steps of acquiring a plurality of proteins by the aid of a protein design template; optimizing each protein, in other words, searching certain rotamers in a rotamerlib, and creating a temporary particle by the aid of each found rotamer; computing the fitness of each temporary particle, and updating local optimal particles and neighbor optimal particles according to the fitness of each temporary particle; updating positions and speeds of all non-temporary particles; comparing the positions of all the neighbor optimal particles to acquire the global optimal positions; searching side-chain conformation combined spaces by the aid of particle swarms to acquire the optimized proteins. Each certain rotamer is the closest to sub-vectors of the corresponding particle which continuously moves in a 4n (four-dimensional) space. The method has the advantages that discrete problems are solved by the aid of pso, and the polarizable force fields are used as evaluation foundations by the aid of the high efficiency of pso; modification on the proteins can be predicted theoretically, and the proteins can be screened theoretically.