The invention relates to a chemical luminescent
protein chip, a kit and a detection method for detecting the
fucose index of seroglycoid, and belongs to a
protein detection technology. The chemical luminescent
protein chip is characterized in that a substrate slide glass of the
protein chip at least comprises a detection subregion, wherein one detection subregion is used for detecting one serum sample; two detection spot regions and one line of contrast spot regions are arranged in the detection subregion; a detection spot formed by a
specific antibody of fixed
alpha fetoprotein is arranged in one detection spot region, and a detection spot formed by fixed lens culinaris
agglutinin is arranged in the other detection spot region; a contrast spot formed by fixed
bovine serum albumin is arranged in each contrast spot region; and the concentrations of substances on all the detection spots in the same detection spot region are equal. The
protein chip, the kit and the method provided by the invention can be used for accurately detecting the
fucose index of the seroglycoid with high
throughput, and have the advantages of high sensitivity, time saving, convenience, economy and the like in clinical application.