Method for detecting ecotoxicity of heavy metal contaminated soil by utilizing luminous earthworms

Abstract

The invention belongs to the technical field of soil detection, and discloses a method for detecting ecotoxicity of heavy metal contaminated soil by using luminous earthworms. The method comprises thefollowing steps: detecting a soil sample by using a soil heavy metal detection device; obtaining the types of the heavy metals contained in the soil of the to-be-detected area; acquiring eisenia fetida from alternanthera philoxeroides, carrying out purification treatment on the eisenia fetida, and removing impurities in the body; measuring an initial fluorescence value of an earthworm body; putting the earthworms into the soil sample for cultivation; collecting the eisenia fetida, secreting mucus, and performing fluorescence value detection to obtain an actual fluorescence value; and calculating to obtain the ecotoxicity of the heavy metal contaminated soil. A principle that the heavy metal inhibits luminescent proteins in the luminous earthworms is utilized, fluorescence values of the mucus generated before and after activity of the luminous earthworms in soil are detected, an average light inhibition rate is calculated, the consumed time is shorter, and the accuracy is higher.

Description

technical field [0001] The invention belongs to the technical field of soil detection, and in particular relates to a method for detecting the ecological toxicity of heavy metal polluted soil by using luminescent earthworms. Background technique [0002] At present: With the development and progress of human science, a large number of heavy metal pollutants enter the soil environment through various channels, resulting in more and more serious problems of heavy metal pollution in soil. The earthworm test is a common method for diagnosing and identifying the ecological effects of soil pollution. Earthworms are complete soil annelids that accumulate heavy metals through the food chain in the soil. The content of heavy metals in earthworms increases with the deepening of heavy metal pollution. They are the most commonly used indicators for monitoring soil heavy metal pollution and evaluating soil quality. Among earthworms, there is a special kind of earthworms that can emit li...

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Problems solved by technology

[0003] In the prior art, there are many studies on earthworm ecotoxicity tests, but most of them focus on the impact of pesticides or heavy metal pollution on the mortality of earthworms or the evasion rate of earthworms. The judgment of soil pollution is relatively vague, and it is impossible to accurately analyze the soil pollution. to determine the pollution

[0004] Through the above analysis, the existing problems and defects of the prior art are: the research of the earthworm ecotoxicity test in the prior art is relatively vague to the judgment of soil pollution, and cannot accurately determine the pollution of the soil

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