Mechanical hybrid rice seed production method utilizing transgenic technology of chloroplasts
A technology of hybrid rice and chloroplast, applied in the direction of plant gene improvement, recombinant DNA technology, botanical equipment and methods, etc., can solve the problems of complicated procedures and inability to produce seeds in large-scale farms, so as to reduce production costs and operation procedures of seed production , the effect of reducing labor intensity
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[0009] experiment procedure:
[0010] 1 Construction of green fluorescent protein gene carrier for chloroplast transformation
[0011] According to the published related sequences, primers were designed respectively, and a series of elements needed to construct rice plastid site-directed integration expression vectors were cloned by PCR technology: plastid ribosomal (16S) RNA operator promoter (Prrn), plastid psbA gene 3' end terminator (psbA3'), aminoglycoside 3'-adenylyltransferase gene (aadA), rice plastid genome high-frequency homologous recombination fragment (psbC / trnG, size 3362bp), and green fluorescent protein gene ( gfp). The rice plastid site-specific integration expression vector (-psbC-Prrn-gfp-aadA-psbA3'-trnG-) was constructed.
[0012] 2 Genetic transformation of rice chloroplasts
[0013] 2.1 Plant material selection
[0014] Rice (Oryza sativa L.) varieties used for genetic transformation of chloroplasts were selected from easily transformed hybrid rice b...
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