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43 results about "Glycine oxidase" patented technology
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Glycine oxidase (EC 1.4.3.19) is an enzyme with systematic name glycine:oxygen oxidoreductase (deaminating). This enzyme catalyses the following chemical reaction glycine + H₂O + O₂ ⇌ glyoxylate + NH₃ + H₂O₂ (overall reaction) (1a) glycine + O₂ ⇌ 2-iminoacetate + H₂O₂ (1b) 2-iminoacetate + H₂O ⇌ glyoxylate + NH₃ This flavoenzyme containing non-covalently bound FAD.
Resistance gene capable of degrading herbicide glyphosate and encoded protein of resistance gene
InactiveCN103421824AIncrease vitalityHigh activityOxidoreductasesGenetic engineeringFunctional identificationNucleotide
The invention belongs to the field of genetic engineering, relates to separation, determinate evolution in vitro and functional identification of a resistance gene capable of degrading herbicide glyphosate efficiently, and further relates to the resistance gene capable of degrading herbicide glyphosate in plants. The nucleotide sequence of the gene B3S1 is as shown in the figure SEQ ID NO: 1; the amino acid sequence of encoded protein of the resistance gene is as shown in the figure SEQ ID NO: 2. According to the invention, the glycine oxidase (thiO) from marine bacteria bacillus cereus (Bacillus cereus) is amplified, and two rounds of random mutagenesis and a round of DNA shuffling test are performed continuously on the glycine oxidase (thiO), and combined with an activity screening technology on the basis of T7 phage pyrolysis-horseradish peroxidase / dianisidine enzyme coupling, the final glyphosate oxidase mutant B3S1 is obtained. The function of the gene and the application approach of the gene in degrading the resistance of herbicide glyphosate are proved in the invention.
Owner:HUAZHONG AGRI UNIV
Homocysteine diagnostic kit and method for measuring homocysteine concentration
InactiveCN101609017AMicrobiological testing/measurementColor/spectral properties measurementsLyaseWavelength
The invention relates to a homocysteine diagnostic kit utilizing technologies of an indirect enzymatic recycling method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring homocysteine concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, pyruvic acid, reduced coenzyme, homocysteine-alpha, gamma-lyase, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen composition, and stabilizer; the colorless reduced form chromogen composition is oxidized into colored dye through a series of enzymatic reactions; moreover, dye content can be measured at the position where wave length is between 400 and 700nm through a visible analyzer so as to directly reflect the homocysteine concentration.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Modified Glycine Oxidase
ActiveUS20160002610A1MeasurementImprove propertiesImmobilised enzymesBioreactor/fermenter combinationsTest sampleThermal stability
The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.
Owner:AJINOMOTO CO INC
Determination method of glycine and kit for determining glycine
InactiveCN102087265ASuitable for testingMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementBiotechnologyAssay
The invention relates to a determination method of glycine content by adopting an enzymatic recycling method, an enzymatic colorimetric method and an enzyme-linked immuno sorbent assay technology, and composition and components of a reagent, and the technical principle of the determination is based on serial catalytic reaction of glycine dehydrogenase, glycine oxidase and alanine dehydrogenase. The invention also relates to a kit for determining the glycine. The determination method has the advantages of high sensitivity and small error. Therefore, the determination method and the kit disclosed by the invention can be widely applied to food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
InactiveCN101324561AMicrobiological testing/measurementColor/spectral properties measurementsFerricytochrome cPeroxidase
The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, ammonia ions, D-lactate dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by utilizing a visible light analyzer and further reflecting the concentration of the D-lactic acid directly.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
InactiveCN101324560AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsFerricytochrome cAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, ammonia ions, D-lactate dehydrogenase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the D-lactic acid.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
5' nucleotidase diagnosis reagent kit and 5' nucleotidase activity concentration determination method
InactiveCN101169376AMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementNucleotidaseAdenosine 5 monophosphate
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Homocysteine diagnostic kit and method for measuring homocysteine concentration
InactiveCN101609013AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsLyaseWavelength
The invention relates to a homocysteine diagnostic kit utilizing technologies of an enzymatic recycling method, an ELISA multiplication method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring homocysteine concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, reduced coenzyme, pyruvic acid, homocysteine-alpha, gamma-lyase, alanine dehydrogenase, glycine oxidase, NADH peroxidase, and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the homocysteine concentration.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Method for determining amino acid (nitrogen) concentration and amino acid (nitrogen) diagnosis/determination reagent kit
InactiveCN101324587AMicrobiological testing/measurementColor/spectral properties measurementsPeroxidaseNitrogen
The invention relates to a kit for diagnosing / mensurating amino acid (nitrogen) by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the amino acid (nitrogen), and belongs to the technology field of medical / food / industrial / agricultural / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, pyruvic acid, amino acid oxidase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the amino acid (nitrogen) directly.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Resistance gene capable of degrading herbicide glyphosate and encoded protein of resistance gene
InactiveCN103421824BIncrease vitalityHigh activityOxidoreductasesGenetic engineeringFunctional identificationNucleotide
The invention belongs to the field of genetic engineering, relates to separation, determinate evolution in vitro and functional identification of a resistance gene capable of degrading herbicide glyphosate efficiently, and further relates to the resistance gene capable of degrading herbicide glyphosate in plants. The nucleotide sequence of the gene B3S1 is as shown in the figure SEQ ID NO: 1; the amino acid sequence of encoded protein of the resistance gene is as shown in the figure SEQ ID NO: 2. According to the invention, the glycine oxidase (thiO) from marine bacteria bacillus cereus (Bacillus cereus) is amplified, and two rounds of random mutagenesis and a round of DNA shuffling test are performed continuously on the glycine oxidase (thiO), and combined with an activity screening technology on the basis of T7 phage pyrolysis-horseradish peroxidase / dianisidine enzyme coupling, the final glyphosate oxidase mutant B3S1 is obtained. The function of the gene and the application approach of the gene in degrading the resistance of herbicide glyphosate are proved in the invention.
Owner:HUAZHONG AGRI UNIV
Reagent (kit) for diagnosing/determining amino acid and method for determining concentration of amino acid
InactiveCN101750319AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsEnzymatic ColorimetryEnzyme catalysis
The invention relates to a reagent (kit) for diagnosing / determining amino acid by using an enzyme colorimetric method and an enzyme link method, and also discloses a method for determining the concentration of the amino acid, a composition and components of the reagent, belonging to the technical field of medicine / food / environmental test determination. The reagent (kit) comprises the main components: a buffer solution, a coenzyme, pyroracemic acid, an amino acid oxidase, a glycine oxidase, an alanine dehydrogenase and a stabilizing agent. A sample and the reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions, and reactants are placed under an ultraviolet / visible light analyzer for detecting the degree of absorbance rise at the dominant wavelength of 340 nm, thereby measuring and calculating the concentration of the amino acid.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration
InactiveCN101324611AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsAdenosine diphosphateAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating inorganic phosphorus by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the inorganic phosphorus, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamine, adenosine diphosphate, pyruvic acid, glutamine synthetase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the inorganic phosphorus.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Adenosine deaminase diagnostic kit and method for measuring activity concentration of adenosine deaminase
InactiveCN101609009AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsWavelengthAlanine dehydrogenase
The invention relates to an adenosine deaminase diagnostic kit utilizing technologies of an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring activity concentration of adenosine deaminase and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, coenzyme, adenosine, pyruvic acid, hydrogen peroxide, glycine oxidase, alanine dehydrogenase, and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the activity concentration of the adenosine deaminase.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Monoamine oxidase diagnostic reagent (kit) and activity concentration measurement method of same
InactiveCN101750314AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsEnzymatic ColorimetryOxidative enzyme
The invention relates to a monoamine oxidase diagnosis / measurement reagent (kit) utilizing an indirect enzymatic recycling amplification method, an enzyme-colorimetric method and an enzyme coupling method, a method for measuring the activity concentration of the monoamine oxidase, a composition and components of the reagent, belonging to the technical field of medical inspection and measurement. The reagent (kit) comprises the following main components: a buffer solution, a coenzyme, an amine compound, pyruvic acid, hydrogen peroxide, a glycine oxidase, an alanine dehydrogenase and a stabilizing agent. The method comprises the following steps of: mixing a sample with the reagent according to a certain volume ratio so as to ensure that the sample and the reagent have a series of enzymatic reactions; placing an obtained reactant under an ultraviolet / visible light analyzer; and detecting the ascending speed of absorbance at a 340nm part of a main wavelength so as to measure the activity concentration of the monoamine oxidase.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Method for detecting glycine content by enzymic method and application thereof
PendingCN110760561AHigh sensitivityRapid and stable detectionMicrobiological testing/measurementBiological material analysisGlyoxylic acidOxidative enzyme
The invention relates to a method for detecting glycine content by an enzymic method and an application thereof, and the method comprises the following steps: determining the glycine content by utilizing a double-enzyme coupling system of glycine oxidase and glyoxylate reductase; according to the method, the defect that a traditional method for coupling glycine oxidase with horseradish peroxidase(HRP) is not suitable for a blood environment is overcome, a double-enzyme coupling method of glycine oxidase and glyoxylate reductase is creatively adopted, glycine is oxidized to form glyoxylic acid, and the content of glycine in the system is rapidly and correctly represented by reducing the content of NADH through a reduction reaction of glyoxylic acid. The method is high in sensitivity, rapidand stable in detection, good in repeatability, simple and convenient to operate, relatively low in cost and relatively wide in application range.
Owner:申友基因组研究院(南京)有限公司 +1
Enzymatic colorimetric method and reagent for measuring concentration of glycocholic acid
InactiveCN108072615AOvercome limitationsResolve accuracyColor/spectral properties measurementsSpecific enzymeGlycocholic acid hydrolase
The invention discloses an enzymatic detection method for measuring content of glycocholic acid in a sample by adopting glycocholic acid hydrolase and glycine oxidase. With the adoption of the method,a brand-new enzymatic colorimetric reagent is further provided and applied to measurement of glycocholic acid in clinical body fluid, and the reagent has the characteristics of being high in specificity and accuracy and convenient to use and adopting easily available materials due to adoption of specific enzyme.
Owner:王学忠
A resistance gene and its encoded protein for degrading the herbicide glyphosate
InactiveCN104450750BHigh degradation activityHigh affinityEnzymesFermentationNucleotideADAMTS Proteins
The invention belongs to the field of genetic engineering, and in particular relates to a resistance gene for degrading herbicide glyphosate and coded protein of the resistance gene. The gene and the protein are screened from a resistance gene which is capable of effectively degrading herbicide glyphosate; the gene is named glyphosate oxidase B4S7 gene, a nucleotide sequence is as shown in SEQ ID NO: 1, and the protein of the protein coded by virtue of the gene is as shown in SEQ ID NO: 2. Through DNA reshuffling on the coding gene of glycine oxidase mutant B3S1 of bacillus cereus as well as the enzyme-coupling activity screening based on T7 bacteriophage lytic-horseradish peroxidase / o-dianisidine, the glyphosate oxidase mutant B4S7 is finally obtained. The resistance gene disclosed by the invention preliminarily verifies the functions of the glyphosate oxidase B4S7 gene as well as application potential of the glyphosate oxidase B4S7 gene in glyphosate-resistance crops.
Owner:HUAZHONG AGRI UNIV
Reagent (kit) for diagnosing/determining amino acid and method for determining concentration of amino acid
InactiveCN101750324AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsEnzymatic ColorimetryAlanine dehydrogenase
The invention relates to a reagent (kit) for diagnosing / determining amino acid by using an enzyme multiplication method, an enzyme colorimetric method and an enzyme link method, and also discloses a method for determining the concentration of the amino acid, a composition and components of the reagent, belonging to the technical field of medicine / food / environmental test determination. The reagent (kit) comprises the main components: a buffer solution, a coenzyme, pyruvic acid, hydrogen peroxide, an amino acid oxidase, a glycine oxidase, an alanine dehydrogenase and a stabilizing agent. A sample and the reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions, and reactants are placed under an ultraviolet / visible light analyzer for detecting the speed of absorbance rise at the dominant wavelength of 340 nm, thereby measuring and calculating the concentration of the amino acid.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Carbon dioxide diagnosis/determination reagent kit and method for determining carbon dioxide concentration
InactiveCN101324597AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsAbsorbanceAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating carbon dioxide by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the carbon dioxide, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, acetic acid, ferrocytochrome b1, ammonia ions, pyruvate oxidase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, an enzymatic reactions occurs, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the carbon dioxide.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Adenosine deaminase diagnosis reagent kit and adenosine deaminase activity and concentration determination method
InactiveCN101169374AFast measurementImprove accuracyMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementAlanine dehydrogenaseMedical testing
The invention relates to a adenylate deaminase diagnostic reagent box utilizing the enzyme cycle amplification, the enzyme-colorimetric and the enzyme-linked techniques, meanwhile, the invention also relates to a method for detecting the activity consistence of the adenylate deaminase, and the compositions and the component of reagent, and belongs to the technical field of medical examination and determination. The reagent box provided by the invention contains buffer solution, reduced coenzyme, adenosine, pyruvic acid, alanine dehydrogenase, glycine oxidase and stabilizer. The sample is mixed with the reagent at certain volume ratio to impel the series of enzymatic reaction between the sample and the reagent, and the reactant is then put under an analyzer of visible light / ultraviolet ray to detect the descending speed of absorbency at the 340nm of the main wavelength, thus, the activity consistence of the adenylate deaminase can be calculated. The invention can completely obtain the needed test result with the help of the analyzer of visible light / ultraviolet ray.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Modified glycine oxidase
ActiveUS9976126B2MeasurementImprove propertiesMicrobiological testing/measurementBiological material analysisTest sampleThermal stability
The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.
Owner:AJINOMOTO CO INC
Inorganic phosphorus (phosphate radical) diagnosis/determination kit and method for determining inorganic phosphorus (phosphate radical) concentration-833
InactiveCN101620097AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsPhosphateEnzymatic Colorimetry
The invention relates to an inorganic phosphorus (phosphate radical) diagnosis / determination kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining inorganic phosphorus (phosphate radical) concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food, and environment. The kit comprises the following main components: buffer solution, coenzyme, pyruvic acid, ammonia chloride, pyruvate oxidase, glycine oxidase, alanine dehydrogenase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the inorganic phosphorus (phosphate radical) concentration.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Manganese ion diagnosis/determination reagent kit and method for determining manganese ion concentration
InactiveCN101324624AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsManganeseAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating manganese ions by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the manganese ions, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, oxaloacetic acid, ammonia ions, oxaloacetic decarboxylase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the manganese ions.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration
InactiveCN101324619AMicrobiological testing/measurementColor/spectral properties measurementsPeroxidaseAdenosine diphosphate
The invention relates to a kit for diagnosing / mensurating inorganic phosphorus by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the inorganic phosphorus, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamine, adenosine diphosphate, pyruvic acid, glutamine synthetase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the inorganic phosphorus directly.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration
InactiveCN101324618AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsAdenosine diphosphateAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating inorganic phosphorus by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the inorganic phosphorus, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, adenosine diphosphate, oxaloacetic acid, ammonia ions, pyruvate carboxylase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the inorganic phosphorus.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Carbon dioxide diagnosis/determination reagent kit and method for determining carbon dioxide concentration
InactiveCN101324607AMicrobiological testing/measurementColor/spectral properties measurementsPeroxidaseAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating carbon dioxide by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the carbon dioxide, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, acetic acid, ferrocytochrome b1, ammonia ions, pyruvate oxidase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the carbon dioxide directly.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Ammonia (ammonia ion) determination method and ammonia (ammonia ion) diagnosis/measurement kit
InactiveCN102297938AMicrobiological testing/measurementColor/spectral properties measurementsElisa kitAlanine aminotransferase
The present invention relates to the measuring method of the content of ammonia (ammonia ion) using enzyme cycle amplification method, enzyme colorimetric method and enzyme-linked method technology, the composition and composition of reagent, the technical principle of its measuring is based on glycine oxidase, alanine aminotransferase 1. The series of catalyzed reactions of glutamate dehydrogenase are completed, and the present invention also relates to a diagnostic / measurement kit for ammonia (ammonia ion). The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Kalium diagnosis / determination reagent kit and method for determining kalium concentration
InactiveCN101329356AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsPotassiumAdenosine diphosphate
The invention relates to a kalium diagnosis / determination kit which uses the techniques of an enzyme circulation amplification method, an enzyme colorimetry method and an enzyme-linked method; simultaneously, the invention also relates to a method principle used for determining kalium consistency, reagent composition and component, belonging to the field of medical / food / environment detection determination technique. The main compositions of the kit of the invention comprise buffer solution, reduction-typed coenzyme, adenosine diphosphate, phosphoenolpyruvate, ammonium ion, pyruvate kinase, alanine dehydrogenase, glycine oxidase, and stabilizer; samples and reagents are mixed according to a certain volume proportion so as to generate a series of enzymatic reactions; subsequently, reaction matters are arranged under an ultraviolet / visible light analyser so as to detect the degree / speed of the absorbency reduction of the main wavelength at the 340nm position, thus calculating the consistency of the kalium.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Ammonia (ammonia ion) determination method and ammonia (ammonia ion) diagnosis/measurement kit
InactiveCN102297957AMicrobiological testing/measurementColor/spectral properties measurementsAlanine aminotransferaseAmmonia
The present invention relates to the measuring method of ammonia (ammonia ion) content using enzyme colorimetric method and enzyme-linked method technology, the composition and composition of reagent, the technical principle of its measuring is based on glycine oxidase, alanine aminotransferase, glutamic acid synthetase The series of catalytic reactions are completed, and the present invention also relates to an ammonia (ammonia ion) diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration
InactiveCN101324620AMicrobiological testing/measurementColor/spectral properties measurementsPeroxidaseAdenosine diphosphate
The invention relates to a kit for diagnosing / mensurating inorganic phosphorus by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the inorganic phosphorus, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamine, adenosine diphosphate, pyruvic acid, glutamine synthetase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the inorganic phosphorus directly.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
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