43 results about "Glycine oxidase" patented technology

The invention relates to a homocysteine diagnostic kit utilizing technologies of an indirect enzymatic recycling method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring homocysteine concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, pyruvic acid, reduced coenzyme, homocysteine-alpha, gamma-lyase, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen composition, and stabilizer; the colorless reduced form chromogen composition is oxidized into colored dye through a series of enzymatic reactions; moreover, dye content can be measured at the position where wave length is between 400 and 700nm through a visible analyzer so as to directly reflect the homocysteine concentration.

The invention belongs to the field of genetic engineering, relates to separation, determinate evolution in vitro and functional identification of a resistance gene capable of degrading herbicide glyphosate efficiently, and further relates to the resistance gene capable of degrading herbicide glyphosate in plants. The nucleotide sequence of the gene B3S1 is as shown in the figure SEQ ID NO: 1; the amino acid sequence of encoded protein of the resistance gene is as shown in the figure SEQ ID NO: 2. According to the invention, the glycine oxidase (thiO) from marine bacteria bacillus cereus (Bacillus cereus) is amplified, and two rounds of random mutagenesis and a round of DNA shuffling test are performed continuously on the glycine oxidase (thiO), and combined with an activity screening technology on the basis of T7 phage pyrolysis-horseradish peroxidase / dianisidine enzyme coupling, the final glyphosate oxidase mutant B3S1 is obtained. The function of the gene and the application approach of the gene in degrading the resistance of herbicide glyphosate are proved in the invention.

The invention relates to an adenosine deaminase diagnostic kit utilizing technologies of an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring activity concentration of adenosine deaminase and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, coenzyme, adenosine, pyruvic acid, hydrogen peroxide, glycine oxidase, alanine dehydrogenase, and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the activity concentration of the adenosine deaminase.

The invention relates to a monoamine oxidase diagnosis / measurement reagent (kit) utilizing an indirect enzymatic recycling amplification method, an enzyme-colorimetric method and an enzyme coupling method, a method for measuring the activity concentration of the monoamine oxidase, a composition and components of the reagent, belonging to the technical field of medical inspection and measurement. The reagent (kit) comprises the following main components: a buffer solution, a coenzyme, an amine compound, pyruvic acid, hydrogen peroxide, a glycine oxidase, an alanine dehydrogenase and a stabilizing agent. The method comprises the following steps of: mixing a sample with the reagent according to a certain volume ratio so as to ensure that the sample and the reagent have a series of enzymatic reactions; placing an obtained reactant under an ultraviolet / visible light analyzer; and detecting the ascending speed of absorbance at a 340nm part of a main wavelength so as to measure the activity concentration of the monoamine oxidase.

The invention belongs to the field of genetic engineering, and in particular relates to a resistance gene for degrading herbicide glyphosate and coded protein of the resistance gene. The gene and the protein are screened from a resistance gene which is capable of effectively degrading herbicide glyphosate; the gene is named glyphosate oxidase B4S7 gene, a nucleotide sequence is as shown in SEQ ID NO: 1, and the protein of the protein coded by virtue of the gene is as shown in SEQ ID NO: 2. Through DNA reshuffling on the coding gene of glycine oxidase mutant B3S1 of bacillus cereus as well as the enzyme-coupling activity screening based on T7 bacteriophage lytic-horseradish peroxidase / o-dianisidine, the glyphosate oxidase mutant B4S7 is finally obtained. The resistance gene disclosed by the invention preliminarily verifies the functions of the glyphosate oxidase B4S7 gene as well as application potential of the glyphosate oxidase B4S7 gene in glyphosate-resistance crops.

The invention relates to a reagent (kit) for diagnosing / determining amino acid by using an enzyme multiplication method, an enzyme colorimetric method and an enzyme link method, and also discloses a method for determining the concentration of the amino acid, a composition and components of the reagent, belonging to the technical field of medicine / food / environmental test determination. The reagent (kit) comprises the main components: a buffer solution, a coenzyme, pyruvic acid, hydrogen peroxide, an amino acid oxidase, a glycine oxidase, an alanine dehydrogenase and a stabilizing agent. A sample and the reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions, and reactants are placed under an ultraviolet / visible light analyzer for detecting the speed of absorbance rise at the dominant wavelength of 340 nm, thereby measuring and calculating the concentration of the amino acid.

The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.

The invention relates to an inorganic phosphorus (phosphate radical) diagnosis / determination kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining inorganic phosphorus (phosphate radical) concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food, and environment. The kit comprises the following main components: buffer solution, coenzyme, pyruvic acid, ammonia chloride, pyruvate oxidase, glycine oxidase, alanine dehydrogenase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the inorganic phosphorus (phosphate radical) concentration.