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Homocysteine diagnostic kit and method for measuring homocysteine concentration

A technology of homocysteine ​​and reagents, which is applied in the field of medical inspection and measurement, and can solve problems such as complex operation, large variation of test data, and long test time

Inactive Publication Date: 2009-12-23
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but its disadvantages are complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] The homocysteine ​​diagnostic reagent of this embodiment is a single reagent, including:

[0105] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0106] Stabilizer 500mmol / L

[0107] Homocysteine-α, γ-lyase 10000U / L

[0108] Alanine dehydrogenase 12000U / L

[0109] Glycine oxidase 12000U / L

[0110] Pyruvate 5mmol / L

[0111] Reduced coenzyme 0.5mmol / L

[0112] Peroxidase 30000U / L

[0113] 4-amino antiarsenic 2mmol / L

[0114] Carbolic acid 10mmol / L

[0115] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0116] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, volume ratio of tested homocysteine ​​sample to reagent is 1 / 25, reaction direction is Positive reaction (rising reaction), the detection method is the rate met...

Embodiment 2

[0119] The homocysteine ​​diagnostic reagent of this embodiment is a dual reagent, including:

[0120] Reagent 1

[0121] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0122] Stabilizer 50mmol / L

[0123] Pyruvate 5mmol / L

[0124] Reduced coenzyme 0.5mmol / L

[0125] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0126] Reagent 2

[0127] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0128] Stabilizer 500mmol / L

[0129] Homocysteine-α, γ-lyase 10000U / L

[0130] Alanine dehydrogenase 12000U / L

[0131] Glycine oxidase 12000U / L

[0132] Peroxidase 30000U / L

[0133] 4-amino antiarsenic 0.6mmol / L

[0134] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0135]Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 546nm, test sub-wavelength 600nm, the volume ratio of the t...

Embodiment 3

[0138] The homocysteine ​​diagnostic reagent of this embodiment is three reagents, including:

[0139] Reagent 1

[0140] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0141] Stabilizer 50mmol / L

[0142] Pyruvate 5mmol / L

[0143] Reduced coenzyme 0.5mmol / L

[0144] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0145] Reagent 2

[0146] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0147] Stabilizer 500mmol / L

[0148] Alanine dehydrogenase 12000U / L

[0149] Glycine oxidase 12000U / L

[0150] Peroxidase 30000U / L

[0151] Dimethylaniline 2mmol / L

[0152] Reagent 3

[0153] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0154] Stabilizer 500mmol / L

[0155] Homocysteine-α, γ-lyase 10000U / L

[0156] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0157] Set on the automatic biochemical analyzer: temperature...

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PUM

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Abstract

The invention relates to a homocysteine diagnostic kit utilizing technologies of an indirect enzymatic recycling method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring homocysteine concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, pyruvic acid, reduced coenzyme, homocysteine-alpha, gamma-lyase, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen composition, and stabilizer; the colorless reduced form chromogen composition is oxidized into colored dye through a series of enzymatic reactions; moreover, dye content can be measured at the position where wave length is between 400 and 700nm through a visible analyzer so as to directly reflect the homocysteine concentration.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnostic kit, and at the same time, the invention also relates to a method for measuring the concentration of homocysteine, which belongs to the technical field of medical examination and determination. Background technique [0002] There are many methods for measuring homocysteine ​​concentration in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, Fluorescence polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] High-performance liquid chromatography (HPLC): It is a classic reference method, but its operation is relatively complicated, the test time is relatively long, and the test data varies greatly. It is gradually adopted by enzyme-linked immunoassay, fluorescence polarization analysis and enzymatically replaced. High-perf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/25G01N33/68C12Q1/527C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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