Vibrio FC509 strain and culturing method and application thereof
A technology of Vibrio and bacterial strains, applied in the field of microorganisms, to achieve the effect of broad application prospects
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Embodiment 1
[0036] Example 1, the acquisition of Vibrio sp. FC509 strain
[0037] A strain of Vibrio sp. FC509 was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on March 12, 2014, with the preservation number CGMCC NO.8913, address: No. 1, Beichen West Road, Chaoyang District, Beijing 3 Institute of Microbiology, Chinese Academy of Sciences.
[0038] Take the soil leachate, add 1 mL of the supernatant to 9 mL of sterile water, dilute to a suitable concentration gradient of 10-1, 10-2, 10-3, 10-4, 10-5, 10-6, and then pass through the conventional Dilution method or streaking method to inoculate and spread on the only carbon source separation medium, culture the bacteria at a constant temperature of 30°C for 1 day, count the colonies, then select colonies with obvious differences in colony morphology, and repeatedly streak and inoculate on the corresponding complete nutrition on the agar plate until a single colony was obtained af...
Embodiment 2
[0044] The cultivation method of Vibrio sp. FC509 bacterial strain, the steps are as follows:
[0045] (1) Take Vibrio sp. FC509 strain and inoculate it into the liquid medium, and cultivate it on a shaking table for 10 hours at a temperature of 28-30°C and a rotation speed of 200rpm to obtain a seed solution;
[0046] (2) Take the seed solution prepared in step (1), inoculate it in the liquid medium at a volume percentage of 5-10%, and expand the culture for 2 hours at a temperature of 25-30°C and a dissolved oxygen of 30%. , to obtain Vibrio sp. FC509 bacteria liquid.
[0047] Preferably according to the present invention, the components per liter of the liquid culture medium are as follows:
[0048] Chondroitin Sulfate 5g, Tryptone 10g, Yeast Extract 5g, NaCl 30g, KH 2 PO 4 3g,K 2 HPO 4 7g, (NH 4 ) 2 SO 4 2g, MgSO 4 ·7H 2 05mg, FeSO 4 ·7H 2 02mg, pH 7.2.
Embodiment 3
[0050] The application of Vibrio sp. FC509 strain in the preparation of glycosaminoglycan lyase, the steps are as follows:
[0051] (1) Take Vibrio sp. FC509 strain and inoculate it into the liquid medium, and cultivate it on a shaker for 16 hours at a temperature of 25-28°C and a rotation speed of 300rpm to obtain a seed solution;
[0052] (2) Take the seed solution prepared in step (1), inoculate it in the liquid medium at a volume percentage of 5-10%, and expand the culture for 5 hours at a temperature of 25-30°C and a dissolved oxygen of 25%. , adding chondroitin sulfate so that the mass concentration of chondroitin sulfate is 0.01-0.02%, and continuing to cultivate for 72 hours to obtain Vibrio sp. FC509 strain fermentation broth;
[0053] (3) Take the fermented broth of Vibrio sp. FC509 strain obtained in step (3), separate the solid from the liquid, take the liquid, add ammonium sulfate to make the mass concentration of ammonium sulfate 80%, collect the precipitate, and...
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