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Novel vibrionaceae vibrio bacterial strain and application thereof

A technology of Vibrio and Vibrio, applied in the field of microorganisms, can solve the problems of poor substrate specificity, limited application of alginate oligosaccharides, low enzyme production and the like

Inactive Publication Date: 2007-02-28
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dozens of alginate lyases have been isolated from bacteria and invertebrates, but the disadvantages of low enzyme production and poor substrate specificity generally limit the application of alginate oligosaccharides

Method used

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  • Novel vibrionaceae vibrio bacterial strain and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The separation of embodiment 1 Vibrio CCTCC M 205031

[0013] Take 1 g of Sargassum, soak it in 100 ml of sterilized seawater, stir for 1 hour, take 1 ml of the suspension and put it into 100 ml of liquid medium, and culture it statically at 25°C for one week. The culture solution was streaked on a solid plate to isolate single clones, and the single clones were purified and cultivated for 5 consecutive generations. Pick a single clone and inoculate it into a test tube containing 3 ml of liquid medium, and culture at 25°C for 12 hours at 200 r / min. The culture solution was transferred into a 250 ml Erlenmeyer flask containing 50 ml of liquid medium at a ratio of 1%, and cultured at 25° C. at 200 r / min for 3 days. The culture solution was centrifuged at 12,000 r / min at 4°C for 10 minutes to remove bacteria, the enzyme activity of the supernatant was measured, and the strain with the highest enzyme activity was selected. One of the bacterial strains produced the highest...

Embodiment 2

[0014] Identification of Example 2 Vibrio CCTCC M 205031

[0015] 1. Morphological characteristics

[0016] The colony of strain CCTCC M 205031 cultured on the conventional Zobell 2216E plate for 24 hours was round, translucent, smooth, with neat edges and a diameter of 1.5 mm; the colony on the conventional TCBS medium was yellow with a diameter of 2.1 mm. The strains are all Gram-negative, rod-shaped, and motile.

[0017] 2. Growth conditions

[0018] Strain CCTCC M 205031 is a facultative anaerobic bacterium. Strain CCTCC M 205031 grows well in the concentration range of 1% to 7% NaCl, the optimum NaCl concentration is 3%, the optimum growth temperature is 25°C, and the optimum growth pH is 6.0.

[0019] 3. Physiological and biochemical characteristics

[0020] Routine biochemical experiments showed that the strain CCTCC M 205031 had a positive oxidase reaction, fermented glucose to produce acid and did not produce gas, and was resistant to Vibrio inhibitor O / 129 (2,4-d...

Embodiment 3

[0025] Strain CCTCC M 205031 is a Gram-negative bacillus, motile, positive for oxidase reaction, fermenting glucose to produce acid but not gas, sensitive to Vibrio inhibitor O / 129, non-luminous, and non-pigment-producing, so the preliminary judgment of the strain CCTCC M 205031 belongs to the genus Vibriosp. of the Vibrio family. According to the comparison of the physiological and biochemical characteristics of this strain with some Vibrio bacteria, CCTCC M205031 is the closest to V.aestuarianus, but it should be pointed out that there are many references to V.aestuarianus in Bergey's Bacteria Identification Manual (Ninth Edition). Physiological and biochemical characteristics are not described, so CCTCC M 205031 may have more similarities and differences with V.aestuarianus. The preparation of embodiment 3 alginate lyase

[0026] (1) Cell culture

[0027] A single clone of Vibrio CCTCC NO: M 205031 was picked and inoculated into a test tube containing 3 ml of liquid mediu...

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Abstract

The invention relates the vibrionaceae vibrio strain and its application. The invention relates the vibrionaceae vibrio strain from gulfweed, and the output of algin lyase made by the vibrionaceae vibrio strain is 2-37 times than that of algin lyase made with now technology. The algin lyase can be used to make algin oligosaccharide.

Description

technical field [0001] The present invention relates to the field of microorganisms. Specifically, the present invention relates to a new Vibrio sp.QY2 strain of the genus Vibrio, whose preservation number is CCTCC NO: M 205031, and the application of the strain to prepare alginate lyase and alginate oligosaccharides. Background technique [0002] Alginate is an acidic marine polysaccharide with rich sources and wide uses, which is derived from brown algae and certain bacteria (including Pseudomonas, nitrogen-fixing bacteria, etc.), composed of β-D-mannuronic acid and α-L-guro Uronic acid is formed by β-1,4 glycosidic bonds, and can be divided into polymannuronic acid, polyguluronic acid and alternating blocks of mannuronic acid and guluronic acid. Recently, the biological activities of alginate oligosaccharides have been continuously discovered, such as anti-tumor, anti-virus, anti-bacterial, anti-cardiovascular disease, etc., and its broad application prospects have attra...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12P19/00C12R1/63
Inventor 于文功韩峰
Owner OCEAN UNIV OF CHINA
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