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5' nucleotidase diagnosis reagent kit and 5' nucleotidase activity concentration determination method

A diagnostic kit, nucleotidase technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, color/spectral property measurement, etc., can solve the problems of strong acid pollution environment, isotope pollution, complex methods

Inactive Publication Date: 2008-04-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The isotope substrate method is complicated and has isotope pollution, and requires an isotope analyzer, so it is difficult to practically popularize and apply
Inorganic phos

Method used

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  • 5' nucleotidase diagnosis reagent kit and 5' nucleotidase activity concentration determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] The 5' nucleotidase diagnostic reagent of this embodiment is a single reagent, comprising:

[0100] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0101] Stabilizer 50mmol / L

[0102] Reduced coenzyme 0.25mmol / L

[0103] Peroxidase 30000U / L

[0104] Adenosine deaminase 6000U / L

[0105] Alanine dehydrogenase 8000U / L

[0106] Glycine oxidase 12000U / L

[0107] Adenosine monophosphate 8mmol / L

[0108] Pyruvate 16mmol / L

[0109] 4-amino antiarsenic 2mmol / L

[0110] Carbolic acid 10mmol / L

[0111] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0112] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the tested 5' nucleotidase sample to the reagent is 1 / 25, and the reaction direction It is a positive...

Embodiment 2

[0115] The 5' nucleotidase diagnostic reagent of this embodiment is a double reagent, comprising:

[0116] Reagent 1

[0117] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0118] Stabilizer 50mmol / L

[0119] Reduced coenzyme 0.25mmol / L

[0120] Adenosine monophosphate 6mmol / L

[0121] Pyruvate 20mmol / L

[0122] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0123] Reagent 2

[0124] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0125] Stabilizer 50mmol / L

[0126] Peroxidase 30000U / L

[0127] Adenosine deaminase 8000U / L

[0128] Alanine dehydrogenase 8000U / L

[0129] Glycine oxidase 16000U / L

[0130] 4-amino antiarsenic 0.6mmol / L

[0131] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0132] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 546nm, test sub-w...

Embodiment 3

[0135] The 5' nucleotidase diagnostic reagent of this embodiment is three reagents, comprising:

[0136] Reagent 1

[0137] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0138] Stabilizer 50mmol / L

[0139] Reduced coenzyme 0.25mmol / L

[0140] Dimethylaniline 2mmol / L

[0141] Reagent 2

[0142] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0143] Stabilizer 50mmol / L

[0144] Adenosine monophosphate 5mmol / L

[0145] Pyruvate 20mmol / L

[0146] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0147] Reagent 3

[0148] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0149] Stabilizer 50mmol / L

[0150] Peroxidase 30000U / L

[0151] Adenosine deaminase 8000U / L

[0152]Alanine dehydrogenase 10000U / L

[0153] Glycine oxidase 10000U / L

[0154] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0155] Set on the autom...

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Abstract

The invention relates to a 5(1) nucleotidase diagnosis box applying the enzyme cycle amplification, the enzyme-colorimetric and the enzyme-linked techniques, meanwhile, the invention also relates to a method for detecting the activity consistence of the 5(1) nucleotidase, and the composition and the compound of reagent, and belongs to the technical field of medical examination and determination. The diagnosis box provided by the invention contains buffer solution, reduced coenzyme, adenosine monophosphate, pyruvic acid, adenylate deaminase, alanine dehydrogenase, glycine oxidase, peroxydase, reduced chromogen combination and stabilizer; the sample is mixed with the reagent at certain volume ration to produce the series of enzymatic reaction, finally the colorless reduced chromogen combination is oxidized into the colored dye, thus, the dye content can be measured at the wavelength between 400 and 700 nm through the analyzer of visible light, therefore, the activity consistence of the 5(1) nucleotidase can be calculated.

Description

technical field [0001] The invention relates to a 5' nucleotidase diagnostic kit, and at the same time, the invention also relates to a method for measuring the activity concentration of 5' nucleotidase, which belongs to the technical field of medical examination and determination. Background technique [0002] Medical research shows that the increase of 5'-nucleotidase (5'NT) is mainly seen in obstructive jaundice, and can also be seen in liver cancer and hepatitis. In cholestasis complicated by cholangitis, primary and secondary biliary cirrhosis and chronic hepatitis, the rate of 5'NT increase is higher than that of alkaline phosphatase; the sensitivity of 5'NT increase in liver tumors and hepatic granulomas higher than alkaline phosphatase. Because there is no physiological increase in 5'-nucleotidase activity, it is more sensitive and specific than alkaline phosphatase for the diagnosis of infantile liver disease and hepatic cholestasis of pregnancy. Therefore, the de...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/25C12Q1/32C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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