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Method for determining amino acid (nitrogen) concentration and amino acid (nitrogen) diagnosis/determination reagent kit

An amino acid and kit technology, which is applied in the field of amino acid diagnosis/determination kits and the determination of amino acid concentration, can solve the problems of high cost of equipment, poor specificity, complicated operation methods, etc.

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing determination methods include methods such as chromatography, electrochemical method, instrumental analysis method (amino acid analyzer), spectrophotometer, etc., and these methods have disadvantages such as comparatively complicated operation methods, poor specificity, or high cost of instruments and equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] The amino acid (nitrogen) diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0103] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0104] Stabilizer 500mmol / L

[0105] Reduced coenzyme 0.25mmol / L

[0106] Pyruvate 10mmol / L

[0107] Amino acid oxidase 10000U / L

[0108] Alanine Dehydrogenase 10000U / L

[0109] Glycine oxidase 10000U / L

[0110] Peroxidase 30000U / L

[0111] 4-Amino arsenic resistance 2mmol / L

[0112] Carbolic acid 10mmol / L

[0113] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, pure water is added and reconstituted before use.

[0114] Set on the automatic biochemical analyzer: the temperature is 37°C, the reaction time is 10 minutes, the main test wavelength is 505nm, the test sub-wavelength is 600nm, the volume ratio of the tested amino acid (nitrogen) sample to the reagent is 1 / 25, and the reaction direc...

Embodiment 2

[0117] The amino acid (nitrogen) diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0118] Reagent 1

[0119] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0120] Stabilizer 50mmol / L

[0121] Reduced coenzyme 0.25mmol / L

[0122] Pyruvate 10mmol / L

[0123] 2,4,6-Tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0124] Reagent 2

[0125] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0126] Stabilizer 500mmol / L

[0127] Amino acid oxidase 10000U / L

[0128] Alanine Dehydrogenase 10000U / L

[0129] Glycine oxidase 10000U / L

[0130] Peroxidase 30000U / L

[0131] 4-Amino arsenic resistance 0.6mmol / L

[0132] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0133] Set on the automatic biochemical analyzer: the temperature is 37°C, the reaction time is 10 minutes, the main test wavelength is 546nm, the test...

Embodiment 3

[0136] The amino acid (nitrogen) diagnosis / measurement reagent of this embodiment is three reagents, including:

[0137] Reagent 1

[0138] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0139] Stabilizer 50mmol / L

[0140] Reduced coenzyme 0.25mmol / L

[0141] Pyruvate 10mmol / L

[0142] 3-Methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0143] Reagent 2

[0144] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0145] Stabilizer 500mmol / L

[0146] Alanine Dehydrogenase 10000U / L

[0147] Glycine oxidase 10000U / L

[0148] Peroxidase 30000U / L

[0149] Dimethylaniline 2mmol / L

[0150] Reagent 3

[0151] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0152] Stabilizer 500mmol / L

[0153] Amino acid oxidase 10000U / L

[0154] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0155] Set on the automatic biochemical analyz...

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Abstract

The invention relates to a kit for diagnosing / mensurating amino acid (nitrogen) by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the amino acid (nitrogen), and belongs to the technology field of medical / food / industrial / agricultural / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, pyruvic acid, amino acid oxidase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the amino acid (nitrogen) directly.

Description

technical field [0001] The invention relates to an amino acid (nitrogen) diagnosis / determination kit, and also relates to a method for determining amino acid (nitrogen) concentration, belonging to the technical field of medical / food / industry / agriculture / environmental inspection and determination. Background technique [0002] The determination of amino acid content is an important measurement item in medicine / food / industry / agriculture / environment. Existing determination methods include chromatography, electrochemical method, instrumental analysis method (amino acid analyzer), spectrophotometer, etc. These methods or operation methods are relatively complicated, have poor specificity, or have disadvantages such as high cost of instruments and equipment. [0003] Amino acids are not a simple substance. 17 kinds of amino acids can be directly determined by an amino acid analyzer (the instrument is expensive and cannot be widely used). For medicine / food / industry / agriculture / envi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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