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Determination method of glycine and kit for determining glycine

A technology of glycine and glycine dehydrogenase, which is applied in the field of glycine determination methods and its kits, and can solve the problems of not being allowed to use

Inactive Publication Date: 2011-06-08
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to my country's "Hygienic Standards for the Use of Food Additives" GB2760, as a food additive, glycine is only allowed to be used in flavoring agents and soy milk, and the maximum limit is 1 gram per kilogram, but it is not allowed to be used in milk-containing beverages

Method used

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  • Determination method of glycine and kit for determining glycine
  • Determination method of glycine and kit for determining glycine
  • Determination method of glycine and kit for determining glycine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] 1) Preparation of standard samples

[0120] Dissolve a certain amount of glycine in water or buffer, and then adjust the concentration of glycine to 1 mmol / L;

[0121] 2) Pretreatment of samples to be tested

[0122] The milk sample is used as the sample to be tested without pretreatment;

[0123] 3) Blank sample

[0124] Described water or buffer solution are used as blank sample, and its glycine concentration is 0 mmol / L;

[0125] B. Preparation of reagent solution:

[0126] The glycine determination reagent of the present embodiment is a single-dose reagent, which contains:

[0127] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0128] Glycerol 1mol / L

[0129] NADP + 1.25mmol / L

[0130] Glycine dehydrogenase 16000U / L

[0131] Glycine oxidase 18000U / L

[0132] Alanine dehydrogenase 12000U / L

[0133] Pyruvate 5mmol / L

[0134] Hydrogen peroxide 4mmol / L.

[0135] All these reagents were dissolved in deionized water according to the abov...

Embodiment 2

[0148] 1) Preparation of standard samples

[0149] Dissolve a certain amount of glycine in water, then adjust the concentration of glycine to 1 mmol / L, as a standard sample;

[0150] 2) Preparation of samples to be tested

[0151] The milk sample is used as the sample to be tested and does not need to be processed;

[0152] 3) Blank sample

[0153] Described water is used as blank sample, and its glycine concentration is 0 mmol / L;

[0154] B. Preparation of reagent solution:

[0155] Glycine Assay Reagent is a two-part reagent containing:

[0156] Reagent 1 with the following composition:

[0157] Phosphate buffer 100mmol / L

[0158] NAD + 2mmol / L

[0159] Pyruvate 5mmol / L

[0160] Hydrogen peroxide 5mmol / L;

[0161] Reagent 2 with the following composition:

[0162] Phosphate buffer 100mmol / L

[0163] Ethylene glycol 5mol / L

[0164] Glycine dehydrogenase 16000U / L

[0165] Glycine oxidase 32000U / L

[0166] Alanine dehydrogenase 40000U / L.

[0167] All these reage...

Embodiment 3

[0184] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:

[0185] B. Preparation of reagent solution:

[0186] Glycine Assay Reagent is a three-part reagent that contains:

[0187] Reagent 1 with the following composition:

[0188] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0189] NAD + 2mmol / L

[0190] Pyruvate 6mmol / L

[0191] Hydrogen peroxide 4mmol / L;

[0192] Reagent 2 with the following composition:

[0193] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0194] Propylene glycol 1mol / L

[0195] Glycine oxidase 28000U / L

[0196] Alanine dehydrogenase 50000U / L;

[0197] Reagent 3 with the following composition:

[0198] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0199] Propylene glycol 2mol / L

[0200] Glycine dehydrogenase 26000U / L.

[0201] All these reagents were dissolved in deionized water according to the above concentrations and prepared for...

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Abstract

The invention relates to a determination method of glycine content by adopting an enzymatic recycling method, an enzymatic colorimetric method and an enzyme-linked immuno sorbent assay technology, and composition and components of a reagent, and the technical principle of the determination is based on serial catalytic reaction of glycine dehydrogenase, glycine oxidase and alanine dehydrogenase. The invention also relates to a kit for determining the glycine. The determination method has the advantages of high sensitivity and small error. Therefore, the determination method and the kit disclosed by the invention can be widely applied to food inspection.

Description

technical field [0001] The invention relates to the technical field of food inspection and determination, more specifically, the invention relates to a glycine determination method and a kit thereof. Background technique [0002] Glycine is a non-essential amino acid. Glycine has a unique sweet taste, which can ease the acid and alkali taste, cover the bitter taste of saccharin added in food and enhance the sweet taste. If the human body consumes too much glycine, not only will it not be absorbed and utilized by the human body, but it will also break the body's absorption balance of amino acids and affect the absorption of other amino acids, resulting in nutritional imbalance and affecting health. Glycine cannot be used by the body, it can only be decomposed and converted into heat energy, which will increase the burden on the liver. The nitrogen-containing compounds in the decomposition products have to be excreted through the urine, which will increase the burden on the ...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/79C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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