Reagent (kit) for diagnosing/determining amino acid and method for determining concentration of amino acid
A concentration determination, amino acid technology, applied in biological testing, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of poor specificity, high cost of equipment and complicated operation methods, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0041] The amino acid diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0042] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0043] Stabilizer 500mmol / L
[0044] Coenzyme 3mmol / L
[0045] Amino acid oxidase 10000U / L
[0046] Glycine oxidase 12000U / L
[0047] Alanine dehydrogenase 12000U / L
[0048] Pyruvate 15mmol / L
[0049] Hydrogen peroxide 12mmol / L
[0050] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0051] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the amino acid sample to be tested is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 1 minute, and the detection time is about 2 minute...
Embodiment 2
[0054] The amino acid diagnosis / measurement reagent of this embodiment is a double reagent, including:
[0055] Reagent 1
[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0057] Stabilizer 50mmol / L
[0058] Coenzyme 3mmol / L
[0059] Pyruvate 15mmol / L
[0060] Hydrogen peroxide 12mmol / L
[0061] Reagent 2
[0062]Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0063] Stabilizer 50mmol / L
[0064] Amino acid oxidase 10000U / L
[0065] Glycine oxidase 12000U / L
[0066] Alanine dehydrogenase 12000U / L
[0067] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0068] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the amino acid sample to be tested to reagent 1 and reagent 2 is 2 / 20 / 5, the...
Embodiment 3
[0071] The amino acid diagnosis / measurement reagents of this embodiment are three reagents, including:
[0072] Reagent 1
[0073] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0074] Stabilizer 50mmol / L
[0075] Coenzyme 3mmol / L
[0076] Pyruvate 15mmol / L
[0077] Hydrogen peroxide 12mmol / L
[0078] Reagent 2
[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0080] Stabilizer 500mmol / L
[0081] Glycine oxidase 12000U / L
[0082] Alanine dehydrogenase 12000U / L
[0083] Reagent 3
[0084] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0085] Stabilizer 500mmol / L
[0086] Amino acid oxidase 10000U / L
[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0088] When measuring amino acid concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorba...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com