30 results about "Complete blood count" patented technology

Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.

The present invention is a flow cytometry-based hematology system useful in the analysis of biological samples, particularly whole blood or blood-derived samples. The system is capable of determining at least a complete blood count (CBC), a five-part white blood cell differential, and a reticulocyte count from a whole blood sample. The system preferably uses a laser diode that emits a thin beam to illuminate cells in a flow cell and a lensless optical detection system to measure one or more of axial light loss, low-angle forward scattered light, high-angle forward scattered light, right angle scattered light, and time-of-flight measurements produced by the cells. The lensless optical detection system contains no optical components, other than photoreactive elements, and does not include any moving parts. Finally, the system uses a unique system of consumable reagent tubes that act as reaction chambers, mixing chambers, and waste chambers for the blood sample analyses. The consumable tubes incorporate reference particles, which act as internal standards to ensure that the dilutions made during processing of the samples have been carried out correctly, and to ensure that the instrument is working properly. The present invention also relates to methods for using the system.

Disclosed in one aspect is a method for performing a complete blood count (CBC) on a sample of whole blood by metering a predetermined amount of the whole blood and mixing it with a predetermined amount of diluent and stain and transferring a portion thereof to an imaging chamber of fixed dimensions and utilizing an automated microscope with digital camera and cell counting and recognition software to count every white blood cell and red blood corpuscle and platelet in the sample diluent / stain mixture to determine the number of red cells, white cells, and platelets per unit volume, and analyzing the white cells with cell recognition software to classify them.

A method of using a variable set from complete blood counts and blood chemistry panels to generate a machine learned algorithm for determining the effectiveness of thiopurine treatment on inflammatory bowel disease (IBD) patients using CART, boosted trees, random forest classification, RuleFit and / or logistic regression analysis.

A method of using a variable set from complete blood counts and blood chemistry panels to generate a machine learned algorithm for determining the effectiveness of thiopurine treatment on inflammatory bowel disease (IBD) patients using CART, boosted trees, random forest classification, RuleFit and / or logistic regression analysis.

The disclosure relates to devices and methods for analyzing blood cells in a sample. In various embodiments, the present disclosure provides devices and methods of performing complete blood count (CBC) testing. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and / or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing blood cells in a sample.

The invention provides an eliminating method of interference of the measurement of hypertriglyceride to the concentration of hemoglobin, comprising the following steps of: (1) carrying out complete blood count to blood containing the hypertriglyceride and obtaining the measuring value, HGB lipemia, of the concentration of the hemoglobin and the hematocrit, HCT lipemia, of the erythrocyte; (2) carrying out low-speed centrifugation to the blood containing the hypertriglyceride in the step (1) and leading the erythrocyte and the blood plasma in the blood to be separated; (3) aspirating the blood plasma separated out in the step (2), carrying out complete blood count to the blood plasma and obtaining the measuring value, HGB lipemia blood plasma, of the concentration of the hemoglobin in the blood plasma; and (4) carrying out correction to the measuring value, HGB lipemia, of the concentration of the hemoglobin obtained in the step (1), obtaining the corrected value of the concentration of the hemoglobin and the corrective formula is as follows: HGB corrected value is equal to HGB lipemia-(HGB lipemia blood plasma minus HGB lipemia blood plasma multiplied by HCT lipemia), in the formula, the HGB lipemia blood plasma multiplied by HCT lipemia is the percentage of the erythrocyte in the blood containing the hypertriglyceride.

This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting and analyzing blood cells in biological samples. A small measured quantity of a biological sample, such as whole blood, is placed in a mixing bowl on the disposable test cartridge after being inserted into the cell analyzer. The analyzer also deposits a known amount of diluent / stain in the mixing bowl and mixes it with the blood. The analyzer takes a measured amount of the mixture and dispenses in a sample cup on the cartridge in fluid communication with an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping as it is transferred into the imaging chamber by the analyzer. Images of all of the cellular components within the imaging chamber are counted and analyzed to obtain a complete blood count.

Disclosed in one aspect is a method for performing a complete blood count (CBC) on a sample of whole blood by metering a predetermined amount of the whole blood and mixing it with a predetermined amount of diluent and stain and transferring a portion thereof to an imaging chamber of fixed dimensions and utilizing an automated microscope with digital camera and cell counting and recognition software to count every white blood cell and red blood corpuscle and platelet in the sample diluent / stain mixture to determine the number of red cells, white cells, and platelets per unit volume, and analyzing the white cells with cell recognition software to classify them.

The disclosure relates to devices and methods for analyzing blood cells in a sample. In various embodiments, the present disclosure provides devices and methods of performing complete blood count (CBC) testing. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and / or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing blood cells in a sample.

The invention discloses a dry blood cell analytical method. The technical principle of the method lies in that a specially-made centrifugal capillary tube is detected by a red light source and a blue light source, and an image is obtained through collection and analyzed, so that all the index parameters of complete blood cell count are obtained; during the analysis, the capillary tube is sequentially irradiated through the light sources of two colors, imaging for the capillary tube is carried out by a camera lens; images are collected by a colored thread CCD image sensor; the complete blood cell count subjected to data processing is displayed on a display screen and supports printout. The method consumes short time, is efficient, and can be used for quick and accurate whole blood cell analysis.

The disclosure relates to devices and methods for analyzing blood cells in a sample. In various embodiments, the present disclosure provides devices and methods of performing complete blood count (CBC) testing. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and / or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing blood cells in a sample.

The invention discloses a detection method for red blood cell immune function, comprising the following steps: (1) heparin anticoagulant blood is taken to carriy out centrifugalization, blood plasma is extracted for spare, and red blood cells are prepared into cell suspension of 1.25 multiplied by 10<7> / mL; (2) yeast is prepared into yeast solution of 1.0 multiplied by 10<8> / mL ; (3) the cell suspension and the yeast solution with the equal volume are mixed to be even, the blood plasma is added / not added, then water bath with the temperature of 37 DEG C is carried out for 30minutes, glutaric dialdehyde is fixed, smear, drying and dying are carried out, 100 red blood cells are counted, red blood cells stuck with two or more than two yeasts are one positive rosette, and the rosette rates of red blood cell C3bR and IC are calculated; the invention uses the self-blood plasma to carry out yeast sensitization with quick taking and using, no complement is inactive, no allogenic blood ingredients interfere, the preparation of the sensitized yeast reagent is not needed, complete blood cells are used for preparing the cell suspension after the blood plasma is extracted, thus truly reflecting the environment condition in the body, and leading the test cost to be reduced obviously and the detected result to be accurate and reliable.

The invention discloses a master control data processing system of a dry type blood cell analyzing apparatus. The master control data processing system comprises a photoelectric sensor 6, a printer 1, a liquid crystal displayer 3 and a master control module 10. Through using the master control data processing system, functions of collecting data, processing images, driving the liquid crystal displayer and the like can be realized; a complete blood cell counting result is obtained after performing data processing on the collected image, displayed on a display screen, printed and output. The master control data processing system disclosed by the invention is equipped with a DSP (Digital Signal Processor) chip which is especially used for digital signal processing and is used for the image processing, so that the system operation speed is further increased, the time spent on analyzing is fully saved, and the detection efficiency is improved.

The invention discloses a cytology counting device which comprises a counting device body. A supporting platform is arranged on the lower side of the counting device body, a wear-resistant pad for preventing excessive wear is arranged on the lower side of the supporting platform, a lighting groove and a power source box for providing a power source are arranged inside the supporting platform, a lighting device is arranged inside the lighting groove, a power source line interface is arranged on the front side of the power source box, a power source line is arranged in the power source line interface, a power source plug and a transformer for regulating voltage are arranged on the power source line, and a display support is arranged on the right side of the counting device body. Compared with existing complete blood cell counting modes, the cytology counting device has the advantage that counting accuracy can be improved greatly; the cytology counting device adopts an integral counting mode, and a mode of using a counting plate for mathematical calculation is not needed, so that counting accuracy is improved greatly; medical staff is needed to implement complete blood cell counting modes, operation inevitably has certain errors, but manual operation needed by the cytology counting device is little, so that errors are extremely few and have no impact on judgment of disease conditions.

The invention provides an eliminating method of interference of the measurement of hypertriglyceride to the concentration of hemoglobin, comprising the following steps of: (1) carrying out complete blood count to blood containing the hypertriglyceride and obtaining the measuring value, HGB lipemia, of the concentration of the hemoglobin and the hematocrit, HCT lipemia, of the erythrocyte; (2) carrying out low-speed centrifugation to the blood containing the hypertriglyceride in the step (1) and leading the erythrocyte and the blood plasma in the blood to be separated; (3) aspirating the bloodplasma separated out in the step (2), carrying out complete blood count to the blood plasma and obtaining the measuring value, HGB lipemia blood plasma, of the concentration of the hemoglobin in the blood plasma; and (4) carrying out correction to the measuring value, HGB lipemia, of the concentration of the hemoglobin obtained in the step (1), obtaining the corrected value of the concentration of the hemoglobin and the corrective formula is as follows: HGB corrected value is equal to HGB lipemia-(HGB lipemia blood plasma minus HGB lipemia blood plasma multiplied by HCT lipemia), in the formula, the HGB lipemia blood plasma multiplied by HCT lipemia is the percentage of the erythrocyte in the blood containing the hypertriglyceride.

The invention provides a method for correcting influence of hemolysis on detection of red blood cell series parameters, and the method comprises the following steps of obtaining a red blood cell countmeasurement value, a hemoglobin concentration measurement value, a blood cell specific product measurement value and an average red blood cell hemoglobin measurement value by complete blood cell count; separating the red blood cells and the plasma to obtain the hemoglobin concentration measurement value in the plasma, obtaining a corresponding correction value, and evaluating the result. The redblood cell series parameters of the hemolyzed blood obtained by the method provided by the invention have high numerical accuracy, and the difference from the true value is less than + / -0.5% on average. The method provided by the invention is simple in operation, and the detecting instrument and centrifuge used are laboratory routine equipment, thereby facilitating the popularization and application in various hospitals. The method provided by the invention requires a short time and can quickly obtain the detection result, that is, saving time and saving cost.

The invention discloses an optical detection system of a dry-type blood cell analyzing device. The optical detection system of the dry-type blood cell analyzing device comprises a red light source 5, a blue light source 7, a lens 8 and a colored linear array CCD (Charged Coupled Device) imaging sensor 9, wherein the two light sources are used for sequentially irradiating a to-be-detected capillary tube, the lens 8 is used for imaging the capillary tube, the colored linear array CCD imaging sensor 9 is used for collecting images, and complete blood cell counting results are obtained through data processing. The optical detection system is suitable for the optical detection of a complete blood cell analyzing device, is fast in speed and has stable performances.

Methods are described for identifying CDI patients as well as CDI patients at risk for recurrence. Embodiments include: (1) flow cytometry of circulating peripheral blood mononuclear cells (PBMC) to phenotype for the less efficient immunoglobulin response to bacterial toxins and surface antigens that characterizes patients who will become recurrent; (2) stratification by means of complete blood count (CBC) using a Coulter counter to detect the differences in lower angle light scatter (LAL), which has a larger range in the recurrent population; and (3) stratification by means of complete blood count (CBC) using a Coulter counter to detect the lower axial light loss (AL2) exhibited in recurrent patients.

The invention is directed to a hypoadrenocorticism diagnostic tool including a computer device for executing a trained machine learning algorithm to analyze bloodwork parameters and determine a hypoadrenocorticism diagnosis based on the bloodwork parameters. The bloodwork parameters may include complete blood count and serum chemistry parameters. The computer device receives the bloodwork parameters associated with a patient and analyzes the bloodwork parameters using the trained machine learning algorithm. The computer device determines the hypoadrenocorticism diagnosis indicating whether the patient is positive or negative for hypoadrenocorticism using the trained machine learning algorithm, and displays the hypoadrenocorticism diagnosis on a graphical user interface.

A microfluidic device (10) for full blood count includes a first measurement channel (11) and a second measurement channel (12) separated from the first measurement channel (11). The microfluidic device (10) furthermore includes a first inlet (13) for providing a whole blood sample to the first and second measurement channel (11, 12), a second inlet (14) for providing a lysis agent for white blood cell count in to the first channel (11), a third inlet (15) for providing a quench solution to the first channel (11), and a fourth inlet (16) for providing a lysis agent for hemoglobin measurement to the second channel (12). A method for forming such a microfluidic device (10) and a method for performing a full blood count test using such a microfluidic device (10) are described.