The invention provides a Chinese medicinal composite disinfectant for the clinical laboratory and a preparation method thereof and relates to the technical field of Chinese medicinal disinfection. Thecomposite disinfectant is prepared from, by weight part, mints, lavender, sophora flavescens, radix sileris, cortex dictamni, terpilenol, chlorhexidine hydrochloride, folium artemisiae argyi, rhizomaatractylodis, acorus calamus, realgar, radix angelicae, aloe, cacti, bitter gourds, lonicera japonica, prismatomeris tetrantra (Roxb.) K. Schum., saururus chinensis, folium isatidis, fresh ginger, filipendula palmata, piper longum L., lead oxide, honeysuckle, honeysuckle stems, houttuynia cordata, rhizoma coptidis, an appropriate amount of sodium citrate solution, an appropriate amount of ethyl alcohol and an appropriate amount of distilled water. The prepared Chinese medicinal disinfectant has the advantages that the bactericidal ability is excellent, the acting speed is high, the stabilityis great, no stimulation is generated on the skin, and the disinfectant easily dissolves in water, produces no residues after use and is safe, free of toxic and side effects and capable of being widely applied to the disinfection of human skin, medical apparatus and instruments, medical occasions and the like in the clinical laboratory .