The invention discloses an
attenuated bacteria vector
tuberculosis vaccine carrying
tuberculosis genes as well as a preparation method and application thereof. The preparation process includes the following steps: firstly, structuring a eukaryotic expression
plasmid containing
mycobacterium tuberculosis Ag85B and ESAT-6Agb5B genes; and secondly, using the
electroporation method to convert the structure eukaryotic expression
plasmid into attenuated
Salmonella typhimurium which can stably express
mycobacterium tuberculosis proteins. The combined utilization of
Salmonella typhimurium SL7207 (pVAX-1-ESAT6-Ag85B) and BCG can achieve greater immune protective effect in resisting
mycobacterium tuberculosis infection than by the BCG only; and the SIgA level of mucosa and serum produced by the
Salmonella typhimurium SL7207 (pVAX-1-ESAT6-Ag85B) and BCG combined is higher than BCG and
DNA vaccines. In addition, the Salmonella typhimurium SL7207 (pVAX-1-ESAT6-Ag85B) has greater immune protective ability than
DNA vaccines or Salmonella typhimurium SL7207 (pVAX-1-Ag85B). The invention has the advantages of simple method, low cost, and convenient use, and can not only
resist Salmonella typhosa infection, but also achieve
immune protection against
mycobacterium tuberculosis infection. Moreover, the stable expression of the
mycobacterium tuberculosis proteins after being immunized can induce mucosal and systematic immune responses, and the invention has remarkable
economic benefits.