Photonic crystal molecularly imprinted hydrogel sensor based on water phase as well as preparation method and detection method of photonic crystal molecularly imprinted hydrogel sensor

A technology of photonic crystals and molecular imprinting, which is applied in the direction of material analysis through optical means, material analysis through observation of the impact on chemical indicators, instruments, etc., can solve the problem that it is difficult to be applied to the detection of protein molecules, and the preparation method has not been reported. , prone to denaturation and other issues, to achieve good selectivity and sensitivity, easy preparation, and maintain activity

Inactive Publication Date: 2021-11-05
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are various preparation methods and detection targets of MIPH, the traditional method of preparing MIPH in an organic phase is difficult to be applied to the detection of specific protein molecules due to the specific three-dimensional structure of proteins and the tendency to denature.
Currently, there is no report on the preparation of photonic crystal molecularly imprinted hydrogel sensors for proteins directly synthesized in aqueous phase by one-step method

Method used

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  • Photonic crystal molecularly imprinted hydrogel sensor based on water phase as well as preparation method and detection method of photonic crystal molecularly imprinted hydrogel sensor
  • Photonic crystal molecularly imprinted hydrogel sensor based on water phase as well as preparation method and detection method of photonic crystal molecularly imprinted hydrogel sensor
  • Photonic crystal molecularly imprinted hydrogel sensor based on water phase as well as preparation method and detection method of photonic crystal molecularly imprinted hydrogel sensor

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Experimental program
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Effect test

Embodiment 1

[0040]1. A photonic crystal molecularly imprinted polymer hydrogel film based on an aqueous phase, characterized in that the hydrogel has a photonic crystal structure of inverse opal, is a highly ordered porous material, and has a photonic energy band And band gap, large specific surface area, can produce Bragg diffraction phenomenon under the irradiation of light of specific wavelength.

[0041] 2. The photonic crystal molecularly imprinted hydrogel film according to claim 1, wherein the template of the inverse opal structure is obtained by a vertical self-assembly method of silica microspheres with a size of 283nm, and the microspheres are closely arranged , structure rules. The hydrogel polymerizes on the surface of the template and wraps the template, and removes the silica microspheres by hydrofluoric acid to obtain a hydrogel with an inverse opal photonic crystal structure.

[0042] 3. The photonic crystal molecularly imprinted hydrogel film according to claim 1, wherei...

Embodiment 2

[0046] 1) Use tetraethyl silicate, ammonia water, water and ethanol to synthesize silica microspheres with a diameter of 283nm, wash the product with ethanol and centrifuge and dry, and the dried product is formulated into silica-ethanol with a mass fraction of 2%. Dispersions;

[0047] 2) Cut the glass sheet into two sizes of 1mm×8mm×76mm and 1mm×10mm×30mm, and use it as slide glass and pressing sheet respectively. Wash with deionized water, soak in piranha solution (98% H2SO4-30% H2O2, 7:3, v / v) for 24 hours, wash with deionized water and ethanol in turn, and dry with nitrogen;

[0048] 3) Insert the glass slide vertically into the dispersion liquid, and use the vertical self-assembly method to assemble the photonic crystal template of the opal structure on the glass surface; the self-assembly temperature is 35°C, and the time is 72h;

[0049] 4) Dissolve 23mg of the target protein in 1.0mL of phosphate buffer (0.2mol / L, pH=6.4), add 15mg of N,N'-methylenebisacrylamide and ...

Embodiment 3

[0055] Detection based on the photonic crystal molecularly imprinted hydrogel sensor prepared in Example 2:

[0056] 1) Dip the hydrogel into lysozyme standard solutions containing different concentration gradients respectively. After incubating at room temperature for 20 min, the wavelength of the Bragg diffraction peak of the hydrogel was measured using an ultraviolet spectrophotometer, and the change in wavelength was plotted against the concentration;

[0057] 2) Immerse the hydrogel in 500 μg / mL lysozyme standard solution, use a UV spectrophotometer to measure the Bragg diffraction peak wavelength of the hydrogel every 2.5 minutes until the wavelength does not change, and plot the wavelength and time ;

[0058] 3) Bovine albumin (Bovine albumin), L-glutamic acid (L-Glu), and Escherichia coli protein extract were selected as the interferents of lysozyme to determine the selectivity of the photonic crystal molecularly imprinted hydrogel membrane.

[0059] as attached im...

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Abstract

The invention researches a photonic crystal molecularly imprinted hydrogel sensor based on a water phase as well as a preparation method and a detection method of the photonic crystal molecularly imprinted hydrogel sensor, and belongs to the field of molecularly imprinted sensing. According to the invention, Lysozyme (Lyz) is selected as a research object, a molecular imprinting technology is combined with photonic crystals, and the photonic crystal molecularly imprinted hydrogel sensor is prepared in a water phase in a simple mode through a novel imprinting method and a novel imprinting system. The preparation method comprises the steps of preparing monodisperse silicon dioxide microspheres by adopting an improved method, then preparing a photonic crystal template by adopting a vertical self-assembly method, polymerizing a polymerization solution on the surface of the template to form a film, removing the photonic crystal template by using hydrofluoric acid, and finally obtaining the photonic crystal molecularly imprinted hydrogel sensor with the inverse opal structure by using the template. The hydrogel sensor has good selectivity and sensitivity, and can be successfully used for detecting lysozyme in an actual sample.

Description

technical field [0001] The invention belongs to the field of molecular imprinting sensing, and in particular relates to an aqueous phase-based photonic crystal molecular imprinting hydrogel sensor and a preparation method and a detection method thereof. Background technique [0002] Lysozyme (Lysozyme, Lyz) is a macromolecular protein with a molecular weight of about 14-15kDa. As an alkaline enzyme, it can hydrolyze mucopolysaccharides in bacteria and then lyse the bacteria, which has a certain antibacterial effect. Lysozyme widely exists in microorganisms, animal and plant tissues, egg whites of birds and poultry, tears, blood and milk of mammals, and milk contains about 13 μg lysozyme per 100 mL. Due to its special antibacterial properties, it can be used as a good additive in baby food and beverages. At present, there has been a practice of increasing the content of lysozyme in milk by inoculating certain specific antigens to cows to improve the quality of milk. [000...

Claims

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Application Information

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IPC IPC(8): G01N21/47G01N21/78C08J5/18C08J9/28C08F220/56C08F222/38C08L33/26
CPCG01N21/4788G01N21/783C08J5/18C08J9/28C08F220/56C08J2333/26C08F222/385
Inventor 罗爱芹刘宗坤侯慧鹏梁阿新刘毅权许立霖王微
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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