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Complex enzyme preparation with high thermal stability and preparing method thereof

A compound enzyme preparation and compound vitamin technology, which is applied in applications, animal feed, animal feed, etc., can solve the problems of reducing the content of antigenic crude protein in the diet, the lack of innate immunity of newborn piglets, and the development of the digestive system. Preserve activity and thermal stability, improve feed utilization, and improve digestion utilization

Inactive Publication Date: 2015-12-09
BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of innate immunity of newborn piglets, the development of the digestive system is not perfect, and the ability to secrete digestive enzymes is weak, direct supply of feed mainly such as corn can easily lead to low feed digestibility, low feed intake, and slow growth of piglets. , high rates of diarrhea
[0004] In order to overcome various problems of suckling pigs after weaning, most enterprises start from supplementary feeding before weaning, food inducement, acidification of rations, use of feed materials with good palatability and easy digestion and absorption, adding exogenous enzymes, and reducing antigens in rations. Improvements and optimizations have been made in many aspects, such as stability, reducing the crude protein content of the diet, and using accelerators, but the actual results are uneven

Method used

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  • Complex enzyme preparation with high thermal stability and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Acquisition of marine Rhodotorula WYN1 and domestication of copper tolerance

[0033] (1) Fresh prawns, starfish, seaweed and other biological samples will be collected from the Yellow Sea and placed in a sterile YEPD medium, and red or pink round colonies will be picked for further purification and culture. According to the accumulation of carotenoid of each type of bacteria, the bacteria with high carotenoid production were selected. Among them, the marine red yeast WYN1, which produces higher carotenoids, has oval cells without pseudohyphae; red colonies are formed on YEPD medium with seawater, with smooth surfaces and neat edges; no seeds are produced on sporulation medium Cystospores, multilateral budding, no spore-bore; no sugar fermentation, no starch-like compounds, different inositol. Based on the above characteristics, it is preliminarily determined to belong to the genus Rhodotorula. Keep the slope in the refrigerator.

[0034] (2) Fermentation cultu...

Embodiment 2

[0051] Example 2 A preparation method of red yeast culture

[0052] Inoculate the marine red yeast WYN1 slant strain in YPD liquid seed medium, and shake culture at 28°C for 36 hours to obtain seed liquid;

[0053] Fermentation culture: The initial pH is 8, the seed culture solution is inoculated into the fermentation medium according to the inoculum amount 5%, the shaker speed is 65r / min, after 12 hours of cultivation at 26°C, the temperature is adjusted to 18°C ​​for low-temperature static culture, and the stirring is stopped. Adjust the pH to 4 and keep it for 3 hours; then increase the temperature from 2°C to 26°C every hour, adjust the pH to 5, add peptone at a weight-to-volume ratio of 2.5% of the fermentation broth, and add yeast powder at a volume of 2%; shaker speed At 80r / min, continue to ferment for 12h;

[0054] After the fermentation, the fermentation broth was coarsely filtered with a pore size of 500 μm, and then concentrated by circulating ultrafiltration at 15°C to ...

Embodiment 3

[0058] Example 3 A composite enzyme preparation and its preparation method

[0059] A compound enzyme, consisting of the following components by weight:

[0060] 100 parts of xylanase, 35 parts of cellulase, 70 parts of β-glucanase, 120 parts of protease, 25 parts of medium temperature α-amylase, 5 parts of complex vitamins, 10 parts of complex trace elements, 15 parts of antimicrobial peptides, 25 parts of red yeast culture;

[0061] Each kilogram of the composite trace elements contains 1000 mg of iodine, 180 mg of potassium, 1800 mg of iron, 80 mg of selenium, 200 mg of cobalt, 145 mg of magnesium, 1400 mg of manganese, 20 mg of chromium, 1100 mg of zinc, and 800 mg of copper; the remainder is a carrier, and the carrier is wood chips;

[0062] The preparation method of the sawdust includes the following steps: removing the debris from sawdust or waste sawdust without mold, disease and insects, placing it in an ultrasonic cleaning machine at 200W, 30KHz for 10 minutes, rinsing, drai...

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Abstract

The invention relates to a complex enzyme preparation with high thermal stability and a preparing method thereof. The complex enzyme preparation is prepared from the following components in parts by weight: 80-120 parts of xylanase, 30-40 parts of cellulase, 40-100 parts of beta-glucanase, 100-150 parts of protease, 20-30 parts of mesothermal alpha-amylase, 3-8 parts of composite vitamin, 10-20 parts of composite trace elements, 10-20 parts of antimicrobial peptide and 20-30 parts of red yeast culture, wherein red yeast is specifically ocean red yeast WYN1 (Rhodotorula sp.WYN1) with a preservation number of CCTCC No. M 2014592. The complex enzyme preparation is rich in carotene and chelating copper, can obviously improve the use rate of fodder as well as thermal stability and preservation activation of the enzyme preparation.

Description

Technical field: [0001] The invention belongs to the technical field of enzyme preparations, and specifically relates to a composite enzyme with good thermal stability and a preparation method thereof. Background technique: [0002] In animal husbandry, suckling pig feeding is the most complicated of all stages of pigs. The consequences of unreasonable nutrient supply are slow growth of pigs, high diarrhea rate and high mortality, which in turn slows the growth of medium and large pigs and prolongs the slaughter time. [0003] However, in order to cut off the vertical transmission of maternal diseases, shorten the reproductive cycle of sows, and extend the breeding life, early weaning technology is widely used in large-scale pig breeding industry. However, due to the lack of innate immunity of newborn piglets, the imperfect development of the digestive system, and the weak secretion of digestive enzymes, direct supply of corn and other feeds can easily lead to low digestibility, l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165A23K1/16A23K1/00
Inventor 李绩
Owner BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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