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Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof

A lectin and microfluidic technology, applied in the preparation of test samples, laboratory containers, chemical instruments and methods, etc., can solve the problems of cumbersome operation steps, achieve enhanced contact area, good reproducibility, good effect

Inactive Publication Date: 2013-10-09
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In J.Chromatogr.B, 831, 2006, Jolita et al. used modified cellulose as a substrate, and used pentaethylenehexamine as a cantilever to graft two kinds of lectins respectively to make a lectin affinity chromatographic column. Glycosyl compounds show high reproducibility, but the method is relatively cumbersome

Method used

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  • Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof
  • Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof
  • Micro-fluidic agglutinin chip for glycosyl separation, and preparation method thereof

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preparation example Construction

[0023] A method for preparing a microfluidic lectin chip for glycosyl separation of the present invention, the specific steps are as follows:

[0024] Step 1, adopt AutoCAD software to draw channel graphics, import the designed channel graphics into Adobe Illustrator and print on the film to make a mask by a high-resolution printer (resolution greater than 3000dpi); wherein, the microstructure lines made by the mask plate The width is 0, the channel width is 10-400 μm, the radius of the four entrances and exits is 20-100 μm, and the length of the micro-column is 20-400 mm; the channel pattern includes the micro-column channel and the sample channel, and the micro-column channel includes the front Part 8, micro-column 4, micro-column channel rear 9, the sample channel includes a sample channel front 10, a micro-column 4, a sample channel rear 11, and the sample channel front 10, micro-column 4, and sample channel rear 11 are located On the same horizontal line, the front part 8...

Embodiment 1

[0034] A method for preparing a microfluidic chip with the function of separating glycosyl compounds of the present invention comprises the following steps:

[0035] Step 1, adopt AutoCAD software to draw channel graphics, import the designed channel graphics into Adobe Illustrator and print on the film to make a mask by high-resolution printer (resolution greater than 3000dpi); Wherein, the graphic line width that described mask plate makes is zero, the channel width is 10 μm, the radius of the reservoir at the end of the channel is 20 μm, and the length of the microcolumn is 20 mm; α is 30°, and β is 40°.

[0036] Step 2: Use standard ultraviolet lithography to etch the microstructure on the mask on the chromium plate, turn on the lithography machine to preheat for 10 minutes, take out the chromium plate under yellow light, and place it on the stage for vacuum adsorption; Spread the mask on the chrome plate, press it with a clean polished glass sheet, perform exposure pre-op...

Embodiment 2

[0044] A method for preparing a microfluidic chip with the function of separating glycosyl compounds of the present invention comprises the following steps:

[0045] Step 1, adopt AutoCAD software to draw channel graphics, import the designed channel graphics into Adobe Illustrator and print on the film to make a mask by high-resolution printer (resolution greater than 3000dpi); Wherein, the graphic line width that described mask plate makes is zero, the channel width is 400 μm, the radius of the reservoir at the end of the channel is 100 μm, and the length of the microcolumn is 400 mm; α is 90°, and β is 60°.

[0046] Step 2: Use standard ultraviolet lithography to etch the microstructure on the mask on the chromium plate, turn on the photolithography machine to preheat for 20 minutes, take out the chromium plate under yellow light, and place it on the stage for vacuum adsorption; The mask is laid flat on the chrome plate, pressed with a clean polished glass sheet, and the ex...

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Abstract

The invention relates to a micro-fluidic agglutinin chip, specifically to a method for preparing and modificating a micro-fluidic chip. The micro-fluidic chip is designed and prepared through a micro electro mechanical technology. Dissolved bacterial cellulose is regenerated in a micro channel to be as a filling material of chip channel columns, and agglutinin is fixed on the filling material surface which is subjected to hydrophilic modification; and according to specific affinity effect of agglutinin to glycosyl, substances containing specific glycosyl on the surface, such as glycoprotein and lipopolysaccharide and the like, are separated. The chip consists of a glass base material, channel graph is etched on the base material by an ultraviolet etching technology, and high-temperature bonding is performed by program temperature control. The chip is provided with an inlet and an outlet of a separation sampling, an inlet and an outlet of the filling material, a bacterial cellulose filled column, a sample micro channel and on-line monitoring points. By taking regenerated bacterial cellulose as the chip filling material, the micro-fluidic agglutinin chip has the main advantages of being excellent in effects of bacterial cellulose, simple in fixing step of agglutinin, convenient to perform spectrum monitor, and substantial in glycosyl separation effect.

Description

technical field [0001] The invention relates to the field of flow control chips, in particular to a lectin chip based on glass material and bacterial cellulose matrix filler, which is applied to the separation and detection of sugar-based compounds and microorganisms, and a preparation method thereof. Background technique [0002] The lectin chip is a new technology for glycomics research, and it is a kind of analysis chip in the microfluidic chip. The research of structural glycomics mainly focuses on mass spectrometry and cutting-edge affinity chromatography technology, but mass spectrometry still has difficulties in distinguishing sugar heterogeneities, and there are limitations in the application of crude extraction samples. Samples are extracted by lectin affinity technology The core glycan spectrum information of lectin can provide sugar structure information in a high-throughput manner. Therefore, the lectin chip can be used as a complementary technology of mass spect...

Claims

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Application Information

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IPC IPC(8): G01N1/28B01L3/00
Inventor 孙东平陈春涛聂英朱春林黄洋杨加志
Owner NANJING UNIV OF SCI & TECH
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