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Thermophilic and colloid degrading Bacillus licheniformis sp. QB26 and its application

A technology of Bacillus licheniformis and QB26, which is applied in the field of microbial biotechnology and environmental biology, and can solve problems such as poor crude oil fluidity and impact on oil recovery

Inactive Publication Date: 2012-08-01
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a heat-resistant and degradable colloidal solution for the increasing shortage of petroleum resources and the continuous increase of exploration costs, especially the problem that the colloid content in heavy oil is too high, which leads to poor crude oil fluidity and affects crude oil recovery. Bacillus licheniformis sp.QB26 and its application in microbial oil recovery

Method used

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  • Thermophilic and colloid degrading Bacillus licheniformis sp. QB26 and its application
  • Thermophilic and colloid degrading Bacillus licheniformis sp. QB26 and its application
  • Thermophilic and colloid degrading Bacillus licheniformis sp. QB26 and its application

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Experimental program
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Effect test

Embodiment 1

[0028] Screening and breeding of the Bacillus licheniformis sp. QB26 strain provided by the invention.

[0029] Get Bohai Oilfield produced water water sample, after shaking evenly, take 10ml with pipette, aseptically inoculate in 100ml inorganic salt basal medium (culture medium composition g / L: colloid 10, Na 2 HPO 4 0.6, KH 2 PO 4 0.2, NaNO 3 2. FeSO 4 0.02, MgSO 4 0.3, Cacl 2 0.01, yeast powder 0.5, distilled water, 1000ml, pH7.2, sterilized at 121°C for 30min), cultured on a shaker at 55°C for 7 days with reciprocating shaking. Dilute the enriched shake flask with sterile water to 10 -5 , 10 -6 , 10 -7 . Spread on the new Wang's + liquid wax and LB plates, culture at 55°C for 2 days, and select strains with different colony shapes and sizes. Observe the colonies that grow. Single colonies were picked and checked for purity using a microscope. Each bacterial colony grown at high temperature was inoculated into inorganic salt sucrose medium (sucrose 10, Na...

Embodiment 2

[0033] The morphological characteristics and physiological and biochemical characteristics of the Bacillus licheniformis sp. QB26 strain provided by the present invention.

[0034] According to the experimental method of "Bergey's Manual of Systematic Bacteriology" (Vol. VIII), the Gram staining, cell size and shape, presence or absence of flagella and spores, growth temperature, growth pH range, and NaCl tolerance were detected. Catalase, V-P, mouse acute oral, casein hydrolysis, gelatin hydrolysis, starch hydrolysis, nitrate reduction to nitrite, cellulose hydrolysis, glucose gas production, tyrosine hydrolysis, phenylalanine dehydrogenase, Yolk lecithinase, NaCl and KCl requirement, allantoin and urea salt requirement, oxidase, lysozyme resistance, D-glucose, L-arabinose, D-xylose, D-mannitol acid production, citrate and propionate requirements etc. experiments.

[0035] The results showed that the strain was positive for Gram staining, and the thallus was short rod-shaped...

Embodiment 3

[0037] Morphological characteristics of Pseudomonas aeruginosa sp. DB strain provided by the present invention.

[0038] Carry out with reference to the experimental method of "Bergey's Manual of Systematic Bacteriology" (Vol.VIII), and detect its Gram staining, thalline size and shape, presence or absence of flagella and spores, and growth temperature.

[0039] The results showed that the strain was negative for Gram staining, and the thallus was short rod-shaped with single flagella at the end, capable of movement, aerobic growth, and no spore production. ), most of the cells are club-shaped or linear, arranged in pairs or short chains. The growth temperature is 25-42°C, the optimum temperature is 37°C

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Abstract

The invention relates to a thermophilic and colloid degrading Bacillus licheniformis sp. QB26 and its application. The Bacillus licheniformis sp. QB26 involved in the invention has a preservation number of CGMCC No. 5250. can Being able to grow in thick oil and colloid under a temperature of 30DEG C-60DEG C, the strain has a degradation rate of over 45% to the colloid (20g / L), and can reduce the absolute content of the colloid in the thick oil (20g / L) by over 10%. The strain and its fermentation liquid can well emulsify colloid and thick oil, and can reduce the thick oil viscosity by over 66% when applied to an inorganic salt medium containing 50% thick oil. Further, the colloid and the thick oil can be degraded, and the thick oil viscosity can be reduced. Therefore, the strain provided in the invention has good effects in microbial enhanced oil recovery, and can enhance crude oil recovery by more than 14%.

Description

technical field [0001] The invention belongs to the field of microbial biotechnology and environmental biotechnology, and in particular relates to a strain of Bacillus licheniformis sp. QB26 and its application in microbial oil recovery. Background technique [0002] At present, with the increasing shortage of oil resources and the continuous increase of exploration costs, the secondary oil recovery technology of pure water injection is gradually unavailable due to its low efficiency (almost 60-70% of the crude oil remains in the oil reservoir after water flooding). To meet people's increasing demand for oil resources. In recent years, the tertiary oil recovery method represented by Microbial Enhanced Oil Recovery (MEOR) technology is gradually receiving widespread attention. Microbe-enhanced oil recovery (MEOR) technology is to use the reproduction activities of microorganisms in the formation and the interaction between their metabolites and crude oil or oil reservoirs to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/10C12R1/385
Inventor 马挺齐义彬李国强吴萌萌
Owner NANKAI UNIV
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