The invention provides a preparation method of a rubber concrete panel with a rough surface, and belongs to the field of compound materials. The method specifically includes the steps that treated stone and rubber compound particles are packed into a mold after being mixed evenly, then the mold is covered by a plastic panel with an area equal to that of an upper mold cavity and the thickness of 2-5 mm, an upper mold panel is closed, at 150-180 DEG C, firstly, preheating is conducted for 30-90 seconds, then mold pressing is conducted at 8-18 MPa, and finally, vulcanization is conducted for 10-40 minutes to prepare the rubber concrete panel with the rough surface. The preparation method of the rubber concrete panel with the rough surface has the beneficial effects that through the method, apart of stone on the rubber concrete surface can be made to be raised, not only is the rubber concrete material surface wear resistance improved, but also a surface friction coefficient of a materialcan be increased, and the anti-skid performance is improved, so that the safety of passengers and driving is improved; meanwhile, mold opening after vulcanization is conducted can be made to be easier.