38results about How to "Plant variety improvement" patented technology

The invention discloses application of a cotton GhLecRK1 gene in plant greensickness resistance. The nucleotide sequence of the cotton GhLecRK1 gene is as shown in SEQ ID NO.1, and the amino acid sequence is as shown in SEQ ID NO.1. The GhLecRK1 gene has higher expression in cotton roots compared with that of other tissues, and the gene is induced and expressed by verticillium dahliae; the gene inthe cotton is silenced, verticillium dahliae is more likely to infect plants, and the greensickness resistance of the cotton is reduced, so that it is indicated that the cotton has the greensicknessresistance, the gene can be applied to creation of a new disease-resistant species, and then plant variety improvement is carried out.

The invention discloses a first nucleotide sequence and protein sequence coded by it of paddy C2H2 type zinc finger protein gene OsZFP18 that belongs to gene engineering field. The OsZFP18 anticipates in the development of the fringe and the salt menace response. The expression of mRNA analysis indicates it is induced by high salt. The invention also discloses the method to improve the salt endurance of the plant by using OsZFP18.

The invention discloses a chrysanthemum stress-resistance transcription factor CmMYB2 as well as a plant expression vector, a construction method and an application thereof, belonging to the field of molecular biology. The expression vector pCAMBIA1301-CmMYB2 is constructed with the construction method which comprises the following steps: inserting the CmMYB2 gene into a colon vector pMD19-Tsimplevector (Takara); and after carrying out enzyme digestion on the CmMYB2 gene by BamHI (Takara) and SacI (Takara), connecting to the BamHI locus and SacI locus of an expression vector pCAMBIA1301. The plant expression vector is used for the genetic transformation of plants, the CmMYB2 gene is subjected to over expression under the starting of CaMV35S to synthesize a great quantity of MYB2 proteins, regulate the expression of a downstream target gene and improve plant stress resistance.

The invention discloses application of a rice trypsin inhibitor protein gene OsBBTI4 to improving the resistance of rice to rice blast. The cDNA nucleotide sequence of the rice trypsin inhibitor protein gene OsBBTI4 is shown as SEQ ID No. 1 or the degenerate sequence of SEQ ID No. 1. A transgenosis experiment proves that the overexpression of the gene improves the resistance of rice to rice blast.Therefore, the gene can serve as a target gene to be transferred into a plant, thus the plant disease resistance can be improved, and plant species improvement can be conducted. The transferred trypsin inhibitor can also protect other proteins from being degraded by inhibiting hydrolysis of protease, and accordingly the plant defense capability is effectively improved.

The invention relates to a Leymus chinensis aquaporin and an encoding gene and application thereof. The current researches of Leymus chinensis are only to perform eating and composition analysis for human or livestock, but the researches in molecular biology aspect, regarding Leymus chinensis as drought tolerant crop are less. The Leymus chinensis aquaporin is the protein with the following amino acid sequences: (1) the amino acid sequence in SEQ ID No:1 of the sequence table; and (2) the protein derived from the protein with the amino acid sequence in SEQ ID No:1, with the same activity of the amino acid sequence in SEQ ID No:1 of the sequence table by replacing, deleting or adding one or more of amino acid residues to amino acid sequence in SEQ ID No:1 of the sequence table. The encoding gene of the Leymus chinensis aquaporin of the invention can be transferred in other plants to increase the drought-resisting property of transgenic plants. The Leymus chinensis aquaporin and encoding gene of the invention are used in plant gene engineering field.

The invention belongs to the technical field of genetic engineering and particularly relates to a coding sequence and an application of an epoxy carotene dioxygenase OsNCED3 gene of rice. A method specifically comprises steps as follows: a nucleotide coding sequence of the OsNCED3 gene is cloned, isogeny comparison of the sequence is performed, space expression modes of different endogenous organsand tissue of the gene of the rice and expression mode change after drought, salt stress and plant hormone treatment are analyzed and identified, a Tilling mutant is obtained on the basis of rice Zhonghua11, molecular identification and stress experiments are performed, gene expression change and resistance change are detected. The OsNCED3 gene can be used for plant variety improvement includingimprovement of rice senescence resistance and improvement of the capability of rice for coping with the dark condition, so that the rice yield is improved.

The invention discloses application a cotton GbCaMBP gene in verticillium wilt resisting of plants. The nucleotide sequence of the cotton GbCaMBP gene is as shown in SEQ ID No. 1, and the amino acid sequence of the cotton GbCaMBP gene is as shown in SEQ ID No. 2. The fact that the verticillium wilt resistance of plants is lowered when the GbCaMBP gene is silenced in cotton and the verticillium wilt resistance of plants is increased when the GbCaMBP gene is overexpressed in Arabidopsis shows that the GbCaMBP gene has an effect of resisting verticillium wilt and can be used for creating disease-resistant new germplasm so as to improve plant varieties.

The invention belongs to the field of molecular biology and discloses an iris ensata thumb cysteine-rich protein gene IlCDT1 as well as a plant expression vector and a construction method thereof. The sequence of the iris ensata thumb cysteine-rich protein gene IlCDT1 is SEQ ID NO.1. IlCDT1 is a new heavy metal resistance gene, and the heavy metal resistance of plants can be improved by the gene. The plant expression vector of the iris ensata thumb cysteine-rich protein gene IlCDT1 is formed by the iris ensata thumb cysteine-rich protein gene IlCDT1 and a plant expression vector. The plant expression vector of IlCDT1 is reported for the first time; the plant expression vector can be directly used for genetic transformation mediated by agrobacterium tumefaciens and creating a new heavy metal resistance germplasm, so that the heavy metal resistance of the plants is improved; the plant expression vector can be used for improving plant varieties.

The invention discloses a flower-specific expression vector of a cucumber CsLOX1 gene, an application of the flower-specific expression vector in transformation of floral organs and belongs to the technical field of biology. The vector is a plant expression vector containing arabidopsis thaliana flower-specific promoter AP3 and the cucumber CsLOX1. By expressing the CsLOX1 gene in arabidopsis thaliana, the obtained CsLOX1 transgenic plant has closed petals, the development of petals is incomplete or is deleted, stamens are degenerated, the number of pistils is increased, ovules and stigmas which abnormally and ectopically grow are obviously visible, which indicates that CsLOX1 plays an important regulatory role in the development of floral organs of cucumber. The novel material prepared through the CsLOX1 gene and capable of varying the floral organs can be applied to breeding of crops and ornamental plants.

The invention provides a chrysanthemum anti-retroelement DgZFP1, a plant expression vector thereof, a construction method thereof and application thereof, belonging to the field of molecular biology. The expression vector pCAMBIA1301-DgZFP1 constructed by the invention is a recombinant plasmid which is obtained by the means that a DgZFP1 gene is inserted into a cloning vector pMD19-T simple vector (Takara), is performed by means of double digestion through BamH1 (Takara) and KpnI (Takara), and is connected with the BamH1 site and the KpnI site of the vector pCAMBIA1301 (Invitrogen). The plant expression vector is used for the genetic transformation of the plant, and the DgZFP1 gene is excessively expressed under the promotion of a CaMV35S promoter, so that a large number of the DgZFP1 protein is synthesized, the expression of the downstream gene is regulated and controlled, and the plant salt tolerance of the plant is improved.

The invention belongs to the technical field of gene engineering, and specifically relates to a coding sequence of an indole-3-acetic acid-amido synthetase gene OsGH3.6 expressed in paddy rice and applications thereof. The applications are as follows: the nucleotide coding sequence of the indole-3-acetic acid-amido synthetase gene OsGH3.6 is cloned; the spatial expression pattern of different endogenous organs and tissues of the gene of paddy rice is analyzed and identified; after treatments such as drought treatment, high salt stress, plant hormone treatment, and the like, the change of expression pattern is analyzed and identified; the tilling mutant of OsGH3.6 is detected, and the root length and output are analyzed.

The invention discloses a peanut R2R3-MYB transcription factor AhMYB31 and an application thereof, and belongs to the field of genetic engineering. The gene has a cDNA sequence of SEQ ID NO.1 and a coding amino acid sequence of SEQ ID NO.2. The AhMYB31 gene in peanuts is reported for the first time, fluorescence quantitative expression analysis indicates that the gene is subjected to high-salty inducible expression and functional verification with trans-arabidopsis, and results indicate that an over-expressed trans-genetic plant shows stronger salt resistance in comparison with a wild plant. Therefore, AhMYB31 can be introduced into a plant as a target gene, so that the salt resistance of the plant can be improved.

The invention discloses application of a rice trypsin inhibitor protein gene OsBBTI4 to improving the resistance of rice to rice blast. The cDNA nucleotide sequence of the rice trypsin inhibitor protein gene OsBBTI4 is shown as SEQ ID No. 1 or the degenerate sequence of SEQ ID No. 1. A transgenosis experiment proves that the overexpression of the gene improves the resistance of rice to rice blast.Therefore, the gene can serve as a target gene to be transferred into a plant, thus the plant disease resistance can be improved, and plant species improvement can be conducted. The transferred trypsin inhibitor can also protect other proteins from being degraded by inhibiting hydrolysis of protease, and accordingly the plant defense capability is effectively improved.

The invention belongs to the field of molecular biology and discloses a salt-resistant gene ZmGnTL of a halophyte zoysia matrella and an expression vector and an application thereof. The sequence of a novel salt-resistant gene ZmGnTl in zoysia matrella is SEQ ID NO.1. The plant expression vector is obtained by performing a recombination reaction with pEarleyGate103 expression vector plasmids after inserting ZmGnTL after double enzyme digestion of BamHI and NotI into a gateway entry vector pENTR1A. The ZmGnTL of zoysia matrella provided by the invention is the novel salt-resistant gene which can improve the salt resistance of the plant and can be applied to creating salt-resistant novel germplasms and improving plant varieties.

The invention relates to the construction of an RNAi (RNA interfere) plant expression vector of a soybean allergy protein gene Gly m Bd 28K, belonging to the filed of molecular biology. Plant vectors PBI (protein-bound iodine) 121 and pCAMBIA3301 are respectively transformed, and are connected to obtain a new vector pGSB. Intron segments of CHAS are amplified by PCR (polymerase chain reaction), and a GUS (glucuronidase) sequence in the vector pGSB is replaced by the amplified intron segments. Primers are designed for amplifying Gly m Bd 28K right interference segments and antisense interference segments and the amplified Gly m Bd 28K right interference segments and antisense interference segments are respectively introduced into the vector pGSBI to obtain the RNAi plant expression vector pYZ of the Gly m Bd 28 K. The Gly m Bd 28K is one of the main soybean allergy proteins, and the soybeans transformed from the RNAi plant expression vector can carry out gene silencing on allergic protein genes. The invention establishes a solid basis for ensuring the safety for eating soybeans by allergic people, and has important theoretical and practical meaning for improving the technological level of national soybean molecular breeding.

Belonging to the field of plant genetic engineering, the invention relates to a tomato non-coding RNA gene SL-LINC2 and application thereof. The cDNA sequence SEQ ID NO.1 of the gene is reported for the first time in tomatoes. Expression analysis indicates that the gene has up-regulated expression after TYLCV (tomato yellow leaf curl virus) induction, PCR and RT-PCR verification are carried out onan obtained transgenic tomato plant, then TYLCV is inoculated for disease resistance evaluation, and the result shows that compared with the control group, the transgenic plant has significantly improved resistance to TYLCV.

The invention discloses a tumorous stem mustard stress resistance gene BjEFh1 as well as a plant expression vector and application thereof. The base sequence of the stress resistance gene BjEFh1 is as shown in SEQ ID NO. 1; the recombinant plant expression vector pCAMBIA1302-BjEFh1 constructed by the invention is formed by connecting the SEQ ID NO. 1 sequence in a plant expression vector pCAMBIA1302; the plant expression vector is used for plant genetic transformation, and the BjEFh1 gene can realize over-expression under promotion of a CaMV35S promoter to synthesize a large quantity of BjEFh1 protein, thus enhancing stress resistance of plant.

The invention relates to a dendranthema dichrum cold-resistant gene DdICE1 as well as a plant expression vector and constructing method thereof, belonging to the field of molecular biology. The sequence of the gene DdICE1 is shown in SEQ ID NO.1, and the expression vector is obtained by inserting the gene DdICE1 between the Sal I and Not I endonuclease sites of a gateway vector pENTRlA and enabling recombined pENTRlA-DdICEl plasmid Nsi I single endonuclease digestion to be linearized and then subjected to LR recombination with a pEarleyGate103 vector plasmid. The dendranthema dichrum cold-resistant gene DdICE1 and plant expression vector provided by the invention can be directly used for carrying out agrobacterium-mediated genetic transformation, creating new cold-resistant germplasm, improving the cold resistance of plants, and improving breeds of the plants.

The invention belongs to the field of molecular biology, and discloses a salt-tolerant gene ZmPDI in a halophyte zoysia matrella as well as a plant expression vector and application thereof. The sequence of the salt-tolerant gene ZmPDI in the zoysia matrella is SEQ ID NO. 1. The plant expression vector is obtained by carrying out double enzyme digestion on ZmPDI through BamHI and EcoRV, then inserting the ZmPDI into a gateway entry vector pENTR1A, and finally subjecting the ZmPDI and a pEarleyGate103 expression vector plasmid to recombination reaction. The ZmPDI in the zoysia matrella, which is provided by the invention, is a novel salt-tolerant gene; the gene can be used for improving the salt tolerance of a plant, and can be applied to the creation of novel salt-tolerant germplasm and the improvement of a plant variety.

The invention belongs to the field of molecular biology, and relates to a silvergrass salt-tolerant gene MsVP2 as well as an expression vector and a construction method thereof. The sequence of the MsVP2 gene is SEQ ID NO.1; and the constructed expression vector pEarleyGate103-MsVP2 is obtained by inserting the MsVP2 gene into a cloning vector pMD10-T simple vector, performing double digestion on the MsVP2 gene by use of Sal I and Not I and then connecting the MsVP2 gene to Sal I and Not I sites of a gateway entry vector pENTR1A, linearizing a pENTR1A-MsVP2 plasmid and then performing LR recombination reaction on the linearized pENTR1A-MsVP2 plasmid and a pEarleyGate103 vector plasmid. The silvergrass MsVP2 provided by the invention is a new salt-tolerant gene, can be used for enhancing the salt resistance of plants and can be applied to new salt-tolerant idioplasm creation and plant breed improvement.

The invention belongs to the technical field of gene engineering, and particularly discloses an Enterobacter glyphosate-tolerant gene argF, and a coding protein and application thereof. The gene is cloned from Enterobacter evolved from glyphosate tolerance, and the nucleotide sequence is disclosed as SEQ ID NO.1. The invention detects that any plant expression vector converter plant cell capable of transforming an exogenous gene argF can be utilized to obtain the argF-expressed transgenic plant of which the glyphosate tolerance is enhanced as compared with the non-transgenic plant. The gene disclosed by the invention can be transformed into a plant as a target gene, thereby enhancing the glyphosate tolerance of the transgenic plant and having important meanings for culturing glyphosate-tolerant crops.

The invention provides a chrysanthemum anti-retroelement DgZFP1, a plant expression vector thereof, a construction method thereof and application thereof, belonging to the field of molecular biology. The expression vector pCAMBIA1301-DgZFP1 constructed by the invention is a recombinant plasmid which is obtained by the means that a DgZFP1 gene is inserted into a cloning vector pMD19-T simple vector (Takara), is performed by means of double digestion through BamH1 (Takara) and KpnI (Takara), and is connected with the BamH1 site and the KpnI site of the vector pCAMBIA1301 (Invitrogen). The plant expression vector is used for the genetic transformation of the plant, and the DgZFP1 gene is excessively expressed under the promotion of a CaMV35S promoter, so that a large number of the DgZFP1 protein is synthesized, the expression of the downstream gene is regulated and controlled, and the plant salt tolerance of the plant is improved.

The invention belongs to the field of molecular biology, and relates to a silvergrass salt-tolerant gene MsVP2 as well as an expression vector and a construction method thereof. The sequence of the MsVP2 gene is SEQ ID NO.1; and the constructed expression vector pEarleyGate103-MsVP2 is obtained by inserting the MsVP2 gene into a cloning vector pMD10-T simple vector, performing double digestion on the MsVP2 gene by use of Sal I and Not I and then connecting the MsVP2 gene to Sal I and Not I sites of a gateway entry vector pENTR1A, linearizing a pENTR1A-MsVP2 plasmid and then performing LR recombination reaction on the linearized pENTR1A-MsVP2 plasmid and a pEarleyGate103 vector plasmid. The silvergrass MsVP2 provided by the invention is a new salt-tolerant gene, can be used for enhancing the salt resistance of plants and can be applied to new salt-tolerant idioplasm creation and plant breed improvement.

The invention belongs to the field of molecular biology, and discloses a chrysanthemum aquaporins coding gene CmAQP, a plant expressing vector thereof and a constructing method. The constructed expression vector pCAMBIA1301-CmAQP is recombinant plasmid obtained by means of inserting CmAQP genes into a simple cloning vector pMD19-T, and connecting the CmAQP genes to BamH I locus and Xba I locus of a vector pCAMBIA 1301 after double digestion by the aid of the BamH I and the Xba I. The plant expression vector is used for plant genetic transformation, the CmAQP genes are promoted by CaMV35S promoter to perform excess expression, a great quantity of CmAQP protein is synthesized, and accordingly germination percentage under salt stress of arabidopsis seeds is increased.

The invention belongs to the field of molecular biology, and discloses a salt-tolerant gene ZmPDI in a halophyte zoysia matrella as well as a plant expression vector and application thereof. The sequence of the salt-tolerant gene ZmPDI in the zoysia matrella is SEQ ID NO. 1. The plant expression vector is obtained by carrying out double enzyme digestion on ZmPDI through BamHI and EcoRV, then inserting the ZmPDI into a gateway entry vector pENTR1A, and finally subjecting the ZmPDI and a pEarleyGate103 expression vector plasmid to recombination reaction. The ZmPDI in the zoysia matrella, which is provided by the invention, is a novel salt-tolerant gene; the gene can be used for improving the salt tolerance of a plant, and can be applied to the creation of novel salt-tolerant germplasm and the improvement of a plant variety.

The invention belongs to the technical field of genetic engineering, specifically a rice epoxy carotenoid dioxygenase OsNCED3 Gene coding sequence and its application. The present invention specifically includes: OsNCED3 Cloning of the nucleotide coding sequence of the gene, homologous comparison of the sequence, analysis and identification of the spatial expression pattern of the gene in different organs and tissues of rice endogenously, and the expression pattern changes after drought, high-salt stress and plant hormone treatment , and obtained the Tilling mutant based on rice Zhonghua11, carried out molecular identification and stress experiments, and detected the changes in gene expression and resistance. the gene OsNCED3 It can be used for plant variety improvement, including improving the anti-aging performance of rice and improving the ability of rice to cope with dark conditions, thereby increasing rice yield.

The invention belongs to the field of molecular biology and discloses a salt-resistant gene ZmDUF1644 of a halophyte zoysia matrella, and an expression vector and an application thereof. The sequence of a novel salt-resistant gene ZmDUF1644 in zoysia matrella is as shown in SEQ ID NO.1. The plant expression vector is obtained by performing a recombination reaction with pEarleyGate103 expression vector plasmids after inserting ZmDUF1644 subjected to double enzyme digestion of BamHI and NotI into a gateway entry vector pENTR1A. The ZmDUF1644 of zoysia matrella provided by the invention is the novel salt-resistant gene which can improve the salt resistance of the plant and can be applied to creating salt-resistant novel germplasms and improving plant varieties.

The invention belongs to the field of molecular biology and discloses a salt tolerance gene ZmPDI in zoysia matrella of the halophyte and a plant expression vector and application of the new salt tolerance gene ZmPDI. The sequence of the salt tolerance gene ZmPDI of the zoysia matrella is SEQ ID NO.1. The plant expression vector is obtained in the manner that BamHI and EcoRV are inserted in a gateway entry vector pENTR1A after double enzyme digestion of the ZmPDI and then are subjected to a recombination reaction with pEarleyGate 103 expression vector plasmids. The zoysia matrella ZmPDI is the new salt tolerance gene, can improve salt tolerance of plants and can be applied to creating of new salt tolerance germplasm and improvement of plant breeds.