38results about How to "Plant variety improvement" patented technology

The invention relates to a Leymus chinensis aquaporin and an encoding gene and application thereof. The current researches of Leymus chinensis are only to perform eating and composition analysis for human or livestock, but the researches in molecular biology aspect, regarding Leymus chinensis as drought tolerant crop are less. The Leymus chinensis aquaporin is the protein with the following amino acid sequences: (1) the amino acid sequence in SEQ ID No:1 of the sequence table; and (2) the protein derived from the protein with the amino acid sequence in SEQ ID No:1, with the same activity of the amino acid sequence in SEQ ID No:1 of the sequence table by replacing, deleting or adding one or more of amino acid residues to amino acid sequence in SEQ ID No:1 of the sequence table. The encoding gene of the Leymus chinensis aquaporin of the invention can be transferred in other plants to increase the drought-resisting property of transgenic plants. The Leymus chinensis aquaporin and encoding gene of the invention are used in plant gene engineering field.

The invention belongs to the technical field of genetic engineering and particularly relates to a coding sequence and an application of an epoxy carotene dioxygenase OsNCED3 gene of rice. A method specifically comprises steps as follows: a nucleotide coding sequence of the OsNCED3 gene is cloned, isogeny comparison of the sequence is performed, space expression modes of different endogenous organsand tissue of the gene of the rice and expression mode change after drought, salt stress and plant hormone treatment are analyzed and identified, a Tilling mutant is obtained on the basis of rice Zhonghua11, molecular identification and stress experiments are performed, gene expression change and resistance change are detected. The OsNCED3 gene can be used for plant variety improvement includingimprovement of rice senescence resistance and improvement of the capability of rice for coping with the dark condition, so that the rice yield is improved.

The invention discloses application of a rice trypsin inhibitor protein gene OsBBTI4 to improving the resistance of rice to rice blast. The cDNA nucleotide sequence of the rice trypsin inhibitor protein gene OsBBTI4 is shown as SEQ ID No. 1 or the degenerate sequence of SEQ ID No. 1. A transgenosis experiment proves that the overexpression of the gene improves the resistance of rice to rice blast.Therefore, the gene can serve as a target gene to be transferred into a plant, thus the plant disease resistance can be improved, and plant species improvement can be conducted. The transferred trypsin inhibitor can also protect other proteins from being degraded by inhibiting hydrolysis of protease, and accordingly the plant defense capability is effectively improved.

Belonging to the field of plant genetic engineering, the invention relates to a tomato non-coding RNA gene SL-LINC2 and application thereof. The cDNA sequence SEQ ID NO.1 of the gene is reported for the first time in tomatoes. Expression analysis indicates that the gene has up-regulated expression after TYLCV (tomato yellow leaf curl virus) induction, PCR and RT-PCR verification are carried out onan obtained transgenic tomato plant, then TYLCV is inoculated for disease resistance evaluation, and the result shows that compared with the control group, the transgenic plant has significantly improved resistance to TYLCV.

The invention relates to a dendranthema dichrum cold-resistant gene DdICE1 as well as a plant expression vector and constructing method thereof, belonging to the field of molecular biology. The sequence of the gene DdICE1 is shown in SEQ ID NO.1, and the expression vector is obtained by inserting the gene DdICE1 between the Sal I and Not I endonuclease sites of a gateway vector pENTRlA and enabling recombined pENTRlA-DdICEl plasmid Nsi I single endonuclease digestion to be linearized and then subjected to LR recombination with a pEarleyGate103 vector plasmid. The dendranthema dichrum cold-resistant gene DdICE1 and plant expression vector provided by the invention can be directly used for carrying out agrobacterium-mediated genetic transformation, creating new cold-resistant germplasm, improving the cold resistance of plants, and improving breeds of the plants.

The invention provides a chrysanthemum anti-retroelement DgZFP1, a plant expression vector thereof, a construction method thereof and application thereof, belonging to the field of molecular biology. The expression vector pCAMBIA1301-DgZFP1 constructed by the invention is a recombinant plasmid which is obtained by the means that a DgZFP1 gene is inserted into a cloning vector pMD19-T simple vector (Takara), is performed by means of double digestion through BamH1 (Takara) and KpnI (Takara), and is connected with the BamH1 site and the KpnI site of the vector pCAMBIA1301 (Invitrogen). The plant expression vector is used for the genetic transformation of the plant, and the DgZFP1 gene is excessively expressed under the promotion of a CaMV35S promoter, so that a large number of the DgZFP1 protein is synthesized, the expression of the downstream gene is regulated and controlled, and the plant salt tolerance of the plant is improved.

The invention belongs to the field of molecular biology, and relates to a silvergrass salt-tolerant gene MsVP2 as well as an expression vector and a construction method thereof. The sequence of the MsVP2 gene is SEQ ID NO.1; and the constructed expression vector pEarleyGate103-MsVP2 is obtained by inserting the MsVP2 gene into a cloning vector pMD10-T simple vector, performing double digestion on the MsVP2 gene by use of Sal I and Not I and then connecting the MsVP2 gene to Sal I and Not I sites of a gateway entry vector pENTR1A, linearizing a pENTR1A-MsVP2 plasmid and then performing LR recombination reaction on the linearized pENTR1A-MsVP2 plasmid and a pEarleyGate103 vector plasmid. The silvergrass MsVP2 provided by the invention is a new salt-tolerant gene, can be used for enhancing the salt resistance of plants and can be applied to new salt-tolerant idioplasm creation and plant breed improvement.

The invention belongs to the field of molecular biology, and discloses a chrysanthemum aquaporins coding gene CmAQP, a plant expressing vector thereof and a constructing method. The constructed expression vector pCAMBIA1301-CmAQP is recombinant plasmid obtained by means of inserting CmAQP genes into a simple cloning vector pMD19-T, and connecting the CmAQP genes to BamH I locus and Xba I locus of a vector pCAMBIA 1301 after double digestion by the aid of the BamH I and the Xba I. The plant expression vector is used for plant genetic transformation, the CmAQP genes are promoted by CaMV35S promoter to perform excess expression, a great quantity of CmAQP protein is synthesized, and accordingly germination percentage under salt stress of arabidopsis seeds is increased.

The invention belongs to the field of molecular biology, and discloses a salt-tolerant gene ZmPDI in a halophyte zoysia matrella as well as a plant expression vector and application thereof. The sequence of the salt-tolerant gene ZmPDI in the zoysia matrella is SEQ ID NO. 1. The plant expression vector is obtained by carrying out double enzyme digestion on ZmPDI through BamHI and EcoRV, then inserting the ZmPDI into a gateway entry vector pENTR1A, and finally subjecting the ZmPDI and a pEarleyGate103 expression vector plasmid to recombination reaction. The ZmPDI in the zoysia matrella, which is provided by the invention, is a novel salt-tolerant gene; the gene can be used for improving the salt tolerance of a plant, and can be applied to the creation of novel salt-tolerant germplasm and the improvement of a plant variety.

The invention belongs to the technical field of genetic engineering, specifically a rice epoxy carotenoid dioxygenase OsNCED3 Gene coding sequence and its application. The present invention specifically includes: OsNCED3 Cloning of the nucleotide coding sequence of the gene, homologous comparison of the sequence, analysis and identification of the spatial expression pattern of the gene in different organs and tissues of rice endogenously, and the expression pattern changes after drought, high-salt stress and plant hormone treatment , and obtained the Tilling mutant based on rice Zhonghua11, carried out molecular identification and stress experiments, and detected the changes in gene expression and resistance. the gene OsNCED3 It can be used for plant variety improvement, including improving the anti-aging performance of rice and improving the ability of rice to cope with dark conditions, thereby increasing rice yield.

The invention discloses a first nucleotide sequence and protein sequence coded by it of paddy C2H2 type zinc finger protein gene OsZFP18 that belongs to gene engineering field. The OsZFP18 anticipates in the development of the fringe and the salt menace response. The expression of mRNA analysis indicates it is induced by high salt. The invention also discloses the method to improve the salt endurance of the plant by using OsZFP18.

The invention belongs to the field of molecular biology and discloses a lotus phytochelatin synthase gene NnPCS1 and a plant expression vector and a construction method thereof. The sequence of the lotus phytochelatin synthase gene NnPCS1 is represented by SEQ ID No.1. The NnPCS1 is a new heavy metal resistance associated gene and can improve the heavy metal resistance in plants. The plant expression vector of the lotus phytochelatin synthase gene NnPCS1 consists of the lotus phytochelatin synthase gene NnPCS1 and the plant expression vector. The plant expression vector of the NnPCS1 is reported for the first time, can be directly used in Agrobacterium tumefaciens-mediated genetic transformation to develop a new heavy metal resistant variety and improve the heavy metal resistance in the plants and can be used for plant variety improvement.