Chrysanthemum anti-retroelement DgZFP1, plant expression vector thereof, construction method thereof and application thereof

Abstract

The invention provides a chrysanthemum anti-retroelement DgZFP1, a plant expression vector thereof, a construction method thereof and application thereof, belonging to the field of molecular biology. The expression vector pCAMBIA1301-DgZFP1 constructed by the invention is a recombinant plasmid which is obtained by the means that a DgZFP1 gene is inserted into a cloning vector pMD19-T simple vector (Takara), is performed by means of double digestion through BamH1 (Takara) and KpnI (Takara), and is connected with the BamH1 site and the KpnI site of the vector pCAMBIA1301 (Invitrogen). The plant expression vector is used for the genetic transformation of the plant, and the DgZFP1 gene is excessively expressed under the promotion of a CaMV35S promoter, so that a large number of the DgZFP1 protein is synthesized, the expression of the downstream gene is regulated and controlled, and the plant salt tolerance of the plant is improved.

Description

[0001] technical field [0002] The invention belongs to the field of molecular biology and relates to chrysanthemum anti-reverse transcription factor DgZFP1 The plant expression vector and construction method and application thereof. [0003] Background technique [0004] Abiotic stresses such as high salinity, drought and low temperature have a great impact on the yield and growth of crops. Therefore, the research on plant stress resistance has always been one of the hotspots in the field of botany research. Chrysanthemum is native to my country and is one of the top ten traditional famous flowers in my country and one of the four major cut flowers in the world. It has high ornamental and economic value and occupies an important position in flower production. However, with the continuous increase of the protected cultivation area of ​​chrysanthemums, soil salinization has gradually become the main limiting factor for the annual supply of chrysanthemums. There are many d...

AI Technical Summary

Problems solved by technology

However, with the continuous increase of the protected cultivation area of ​​chrysanthemums, soil salinization has gradually become the main limiting factor for the annual supply of chrysanthemums.

There are many deficiencies in the current soil improvement measures. At the same time, the huge consumption of water resources has become the bottleneck of the current chrysanthemum industry development.

Breeding for stress resistance of chrysanthemums has always been one of the important topics for horticultural workers at home and abroad. Given that stress resistance is a quantitative trait controlled by multiple genes, conventional breeding methods such as cross breeding are inefficient.

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