Energy crop silvergrass salt-tolerant gene MsVP2 as well as plant expression vector and construction method thereof

Abstract

The invention belongs to the field of molecular biology, and relates to a silvergrass salt-tolerant gene MsVP2 as well as an expression vector and a construction method thereof. The sequence of the MsVP2 gene is SEQ ID NO.1; and the constructed expression vector pEarleyGate103-MsVP2 is obtained by inserting the MsVP2 gene into a cloning vector pMD10-T simple vector, performing double digestion on the MsVP2 gene by use of Sal I and Not I and then connecting the MsVP2 gene to Sal I and Not I sites of a gateway entry vector pENTR1A, linearizing a pENTR1A-MsVP2 plasmid and then performing LR recombination reaction on the linearized pENTR1A-MsVP2 plasmid and a pEarleyGate103 vector plasmid. The silvergrass MsVP2 provided by the invention is a new salt-tolerant gene, can be used for enhancing the salt resistance of plants and can be applied to new salt-tolerant idioplasm creation and plant breed improvement.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a grass salt-tolerant gene MsVP2, a plant expression vector and a construction method thereof. Background technique [0002] Salt stress is an important stress factor affecting plant growth and development, and a key factor limiting the efficient use of saline-alkali land in my country. Therefore, the study of salt tolerance has become a hot field. Grass (Miscanthus sacchariflora) is a plant of the Gramineae genus Miscanthus Anderss. As an important energy plant native to my country, it has high development and utilization value. ) and other materials have relatively limited salt tolerance, which greatly affects their cultivation and application in saline-alkali land. Given that salt tolerance is a quantitative trait controlled by multiple genes, conventional breeding methods such as cross-breeding are inefficient. Existing studies have shown that the molecular breeding of high-e...

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Problems solved by technology

Grass (Miscanthus sacchariflora) is a plant of the Gramineae genus Miscanthus Anderss. As an important energy plant native to my country, it has high development and utilization value. ) and other materials have relatively limited salt tolerance, which greatly affects their cultivation and application in saline-alkali land

Given that salt tolerance is a quantitative trait controlled by multiple genes, conventional breeding methods such as cross-breeding are inefficient

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