The invention relates to a timing synchronization method under a high-speed mobile condition. The timing synchronization method under the high-speed mobile condition includes the steps: 1, constructing space base vectors of right crossing angle domains, 2, carrying out projection on the right crossing angle domains of received signals, 3, carrying out rough timing synchronization on the right crossing angle domains, 4, carrying out equivalent Doppler estimate and compensation on the right crossing angle domains, 5, carrying out fine timing synchronization on the right crossing angle domains, and 6, carrying out fine synchronized signal combination and timing judgment on each right crossing angle domain space. According to the timing synchronization method under the high-speed mobile condition, the process of timing synchronization is divided into the rough timing synchronization and the fine timing synchronization, reference synchronization frame header is adopted to carry out frame timing synchronization, and the reference synchronization frame header comprises Q short training sequences with the cycle length of D and one long training sequence with the length of K. A first part of the reference synchronization frame header is mainly used for the rough timing synchronization and frequency synchronization, and a second part of the reference synchronization frame header is mainly used for the fine timing synchronization. When the timing synchronization method under the high-speed mobile condition is used for frequency offset estimation, acquired estimate range is large, estimate accuracy is high, and the function of noise suppression of a synchronizing system can be further improved.