30 results about "Streptomyces clavuligerus" patented technology

A new antibacterially active agent has been isolated from Streptomyces clavuligerus. This new compound which is designated clavulanic acid has the formula (I): In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of beta -lactam antibiotics against many beta -lactamase producing bacteria.

Genetically engineered Streptomyces clavuligerus strains with improved capabilities to produce clavulanic acid are provided. The strains are genetically engineered by disrupting newly identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes. This results in an increased intracelluar pool of the clavulanic acid precursor D-glyceraldehyde-3-phosphate (D-G3P), and increased clavulanic acid production. Clavulanic acid production may be further increased by supplying arginine to the medium in which the S. clavuligerus is grown.

The invention discloses a Streptomyces clavuligerus strain with high production of clavulanic acid and a stress breeding method and application thereof, and belongs to the technical field of biology.The Streptomyces clavuligerus strain with high production of clavulanic acid disclosed herein is collected in China Center for Type Culture Collection on February 25th, 2019 under CCTCC No. 2019110. The Streptomyces clavuligerus strain B-77 with high production of clavulanic acid is cultured by adding stress materials (penicillin and Klebsiella) into a culture medium under suitable adding mode ofthe stress materials, suitable adding time, suitable culture conditions, suitable separate culture process of the strain and the like. The strain acquired via the method herein has good genetic stability; the ability of the strain to produce clavulanic acid is improved significantly; the yield of clavulanic acid is high, and titer is high.

Genetically engineered Streptomyces clavuligerus strains with improved capabilities to produce clavulanic acid are provided. The strains are genetically engineered by disrupting newly identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes. This results in an increased intracelluar pool of the clavulanic acid precursor D-glyceraldehyde-3-phosphate (D-G3P), and increased clavulanic acid production. Clavulanic acid production may be further increased by supplying arginine to the medium in which the S. clavuligerus is grown.

The present invention relates to a process of producing a monoterpene and / or derivatives thereof. The process comprises the steps of: a) providing a host microorganism genetically engineered to express a bacterial monoterpene synthase (mTS); and b) contacting geranyl pyrophosphate (GPP) with said bacterial mTS to produce said monoterpene and / or derivatives thereof. The present invention also relates to a microorganism for use in producing a monoterpene and / or derivatives thereof and a recombinant microor-ganism adapted to conduct the step of converting geranyl pyrophosphate (GPP) into a monoterpene and / or derivatives thereof by ex-pression of a bacterial mTS. It was shown to produce 1,8 cineole using 1,8 cineole synthase and to produce linalool using linalool synthase, both from Streptomyces clavuligerus.

The invention discloses a corn steep liquor fermentation-enzyme catalysis method, which is used to synthesize phenylacetyl-7-amino-3- desacetoxycephalos-porin acid. Streptomyces clavuligerus with an expandase gene is used to make strain transformation to corn steep liquor to ensure that the corn steep liquor is made into fermentation ring enlargement in a culture medium and is reacted with phenylacetic acid added into the culture medium to generate the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid. Water solution of the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid is obtainedthrough organic solvent extraction and water back extraction. Acetyl transferase and nutrition content are added into the water solution to form an enzyme reaction culture medium and finish a biochemical reaction under appropriate condition, and the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid can be produced after side chains are removed.

The method for producing potassium clavulanate by solid-state continuous fermentation involving inert carrier adsorption in the present invention: the Streptomyces clavulus seed liquid and the Streptomyces clavulus fermentation medium are dispersed from the top of the fermenter through a distribution plate and flow down to the inert carrier successively, and the seed liquid is filled in In the carrier, the fermentation medium is covered on the carrier to form a liquid film, and the mycelium of the seed liquid grows and reproduces on the liquid film to produce fermentation metabolites. At the same time, sterile air flows from the bottom of the tank through the distribution plate to provide oxygen for fermentation; Adding and overloading of carrier adsorption, fermentation metabolites flow out of the tank; then exchanged with basic anion resin, 5 times the amount of resin containing 1.5% lithium chloride 95% ethanol solution to obtain potassium clavulanate; fermentation process The medium fermentation medium is continuously pumped in and out, and the fermentation metabolites can be continuously obtained with only one inoculation of the strain, realizing continuous fermentation; the fermentation process does not need to be stirred, the heat and mass transfer is good, and the energy consumption is low. Potassium clavulanate has a high yield.

The invention discloses Streptomyces clavuligerus, its application, a fermentation medium and a preparation method of clavulanic acid; the Streptomyces clavuligerus is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.19076; Disclosed is the application of Streptomyces clavulanic acid in the production of clavulanic acid; the preparation method of clavulanic acid is disclosed comprising: fermenting the Streptomyces clavulanic acid to obtain clavulanic acid; the Streptomyces clavulanicus has good genetic stability, and the output of clavulanic acid is large , high potency.

The invention belongs to the field of microbial engineering, provides a superior streptomyces clavuligerus strain that is used for clavulanic acid production, a strain preparation method and application of the strain, and concretely relates to a method, which obtains high expression of key enzyme in the arginine synthesizing process by increasing the copy number of clavulanic acid synthesizing precursor of the streptomyces clavuligerus strain that is key enzyme gene argG in the arginine synthesizing process, and then obtains the streptomyces clavuligerus strain LNSC-2 with high yield of clavulanic acid. Compared with the original strains, the superior streptomyces clavuligerus strain that is provided by the invention can improve the yield of the zymolytic clavulanic acid production by over 20 percent.

The invention discloses a genetically engineered bacterium for converting cephalosporin C and a preparation method thereof. The genetically engineered bacterium integrates an expression cassette of cmcJ gene into the genome of a wild strain of Streptomyces clavuligerus (ATCC27064). It is verified by cephalosporin C conversion that the genetically engineered bacterium is advantaged in that the conversion rate from cephalosporin C to hydroxy cephalosporin C is improved from 57.0% of a wild strain to 67.4% of a recombinant mutant, and the conversion rate of methoxy cephalosporin C is improved from 43.1% of the wild strain to 51.9% of the recombinant mutant.

The invention discloses a streptomyces clavuligerus for high-yield clavulanic acid, and application of the streptomyces clavuligerus. The streptomyces clavuligerus for high-yield clavulanic acid is specifically a streptomyces clavuligerus 16CF10 of which the preservation number is CGMCC No.7915 in the China General Microbiological Culture Collection Center. According to the invention, a streptomyces clavuligerus 70116 is taken as a starting strain, is subjected to metabolic engineering modification and is mutated to obtain the streptomyces clavuligerus 16CF10 CGMCC No.7915. Experimental results show that the amount of clavulanic acid produced by the strain cultivated through fermentation reaches 2.25 g / L of fermentation liquor, and is improved by about 51% compared with that the streptomyces clavuligerus 70116 serving as the starting strain, so that the streptomyces clavuligerus 16CF10 CGMCC No.7915 has a broad industrial application prospect.

The invention discloses streptomyces clavuligerus and a use method of streptomyces clavuligerus in melanin production. The use method comprises the following steps of carrying out strain activation on streptomyces clavuligerus preserved by refrigeration, then carrying out propagation culture, inoculating a fermentation culture liquid with the strain liquid, carrying out fermentation culture to obtain a fermentation broth, separating a melanin product from the fermentation broth, and drying the melanin product. The strain culture liquid is inoculated with streptomyces clavuligerus and the streptomyces clavuligerus strain is subjected to propagation culture so that the strain liquid is obtained, the strain liquid is transferred to the fermentation culture liquid and is subjected to fermentation culture so that the fermentation broth is obtained, and the melanin product is separated from the fermentation broth and then is dried. The use method has simple processes, can be operated conveniently and can rich melanin production bacterium strain resources. The melanin product has stable performances and has the highest yield of 0.85g / L. The use method provides an efficient and stable method for melanin production and fills in gap of use of microbial strain in melanin production.

The invention belongs to the field of microbial engineering, provides a streptomyces clavuligerus strain LNSC with low oxygen consumption and high fermentation yield, a strain preparation method and application of the strain, and particularly relates to a method. The method utilizes genetic engineering methods, researches the application aspect of vitreoscilla hemoglobin gene (vhb), transfers thegene into the streptomyces clavuligerus culture that is used for clavulanic acid production, obtains high expression of VHB protein, thus improving the production characteristics of the streptomyces clavuligerus, and lowering the energy consumption of zymolytic production.

The invention discloses a high-activity aminoacetylase clavulanate streptomyces clavuligerus strain. The clavulanate streptomyces clavuligerus strain is clavulanate streptomyces B02-246, is preserved in the China general microbiological culture collection center (CGMCC) on October 13, 2015 and has a preservation number of CGMCC NO. 11484. A sequential error-prone PCR method is used for in-vitro mutation of a gene of the aminoacetylase clavulanate, an aminoacetylase clavulanate-less strain is used as a starter, through a conjugational transfer technology, a clavulanate streptomyces gene-containing mutant library is built, a positive mutant is screened, and through fermentation verification and subculture, the clavulanate streptomyces B02-246 with stable genetic characters is obtained. The strain has a clavulanic acid synthesis level 1.3 times higher than that of the original strain B02 and can be used for industrial fermentation production.