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A kind of Streptomyces clavulatus and its application method in producing melanin

A technology of Streptomyces coryneformis and an application method, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as lack of microbial strains, and achieve the effects of convenient operation, simple method and stable performance

Inactive Publication Date: 2020-04-07
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the embodiments of the present invention is to provide a method for the application of Streptomyces clavulicularis in the production of melanin, aiming to solve the problem of lack of existing microbial strains in the production of melanin

Method used

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  • A kind of Streptomyces clavulatus and its application method in producing melanin
  • A kind of Streptomyces clavulatus and its application method in producing melanin
  • A kind of Streptomyces clavulatus and its application method in producing melanin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, strain isolation and identification:

[0037] (1) Strain isolation:

[0038] Soil samples were collected from different locations in Jiaxing Park. When sampling, the soil at a depth of 5cm to 20cm below the soil surface was selected, and brought back to the laboratory in a sterile paper bag; after air-drying and grinding, 1g of the soil sample was added to 99mL of deionized water , to make a soil suspension, and successively diluted to 10 -5 、10 -6 ; take 10 -5 、10 -6 0.1mL of the diluent was spread on the screening medium respectively, cultured at 37°C, and the colonies with early melanin production time and dark color were picked, and then separated and purified by multiple streaks to obtain a single colony;

[0039]The formula of the screening medium is: beef extract 3g, peptone 10g, NaCl 5g, agar 15-20g, pH7.0-7.2, water 1000mL;

[0040] (2) Morphological identification:

[0041] The selected melanin-producing single colony was inoculated in Gaos...

Embodiment 2

[0047] Embodiment 2, bacterial strain fermentation produces melanin:

[0048] (1) Pick colonies from the test tubes where the strains are stored for strain activation, and culture them at 37°C for 48 hours; the formula for the activation medium is:

[0049] (2) pick a single bacterium colony on the activated plate and transfer it to the seed culture solution for proliferation and culture, cultivate it at 37°C for 48 hours, and the rotation speed is 200r / min; the formula of the seed culture solution is (g / L): soluble starch 2g, KNO 3 0.1g, K 2 HP0 4 0.05g, NaCl0.05g, MgSO 4 ·7H 2 O0.05g, FeS0 4 ·7H 2 00.01g, pH7.2-7.4.

[0050] (3) Inoculate the seed liquid into the fermentation culture liquid for fermentation and culture, and cultivate it on a shaking table at 28°C for 7 days at a rotation speed of 200r / min until the fermentation liquid is black;

[0051] (4) Take the black fermented liquid and centrifuge at 10000r / min, take its supernatant, concentrate by rotary evapo...

Embodiment 3

[0052] Example 3, obtaining melanin basic data:

[0053] Carry out a series of tests to the melanin sample that embodiment 2 prepares to obtain, to obtain the basic data of this melanin:

[0054] (1) Structural characterization:

[0055] The melanin was subjected to ultraviolet-visible full-band scanning and infrared spectrum analysis, and the results were as follows: Figure 4 and as shown in Figure 5;

[0056] Depend on image 3 It can be seen that the melanin has a strong absorption peak at about 240nm, and there is no characteristic peak in the visible light region, and the absorbance decreases with the increase of the wavelength. The results are consistent with the literature (Zheng Chenna, Fang Baishan, Luo Juxiang, etc. 4. Extraction and physicochemical properties of melanin. The melanin produced by streptomycin G-HD-4 in Huaqiao University (Natural Science Edition), 2009, 30(3):292-296.) is similar;

[0057] Depend on Figure 5 It can be seen that the infrared spe...

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Abstract

The invention discloses streptomyces clavuligerus and a use method of streptomyces clavuligerus in melanin production. The use method comprises the following steps of carrying out strain activation on streptomyces clavuligerus preserved by refrigeration, then carrying out propagation culture, inoculating a fermentation culture liquid with the strain liquid, carrying out fermentation culture to obtain a fermentation broth, separating a melanin product from the fermentation broth, and drying the melanin product. The strain culture liquid is inoculated with streptomyces clavuligerus and the streptomyces clavuligerus strain is subjected to propagation culture so that the strain liquid is obtained, the strain liquid is transferred to the fermentation culture liquid and is subjected to fermentation culture so that the fermentation broth is obtained, and the melanin product is separated from the fermentation broth and then is dried. The use method has simple processes, can be operated conveniently and can rich melanin production bacterium strain resources. The melanin product has stable performances and has the highest yield of 0.85g / L. The use method provides an efficient and stable method for melanin production and fills in gap of use of microbial strain in melanin production.

Description

technical field [0001] The invention belongs to the technical field of melanin-producing bacteria, in particular to a Streptomyces clavulosa and its application method in producing melanin. Background technique [0002] Melanin refers to the substance that can color the mordant, and is widely used in production, life and scientific research. Commonly used pigments can be roughly divided into two categories: synthetic pigments and natural pigments. Synthetic pigments are organic pigments produced by chemical synthesis methods, mainly made of aniline dyes separated from coal tar; natural pigments usually refer to the use of substances that exist in nature or secondary metabolites produced by cultivation methods Processed pigments. [0003] Compared with synthetic pigments, natural pigments are non-toxic and harmless to the human body, have high nutritional value, and have some important biological activities. Therefore, the development and application of non-toxic and harmle...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/465
Inventor 刘晓侠王玉洁孙诗清尤忠毓徐微
Owner JIAXING UNIV
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