Process for preparation of clavulanic acid employing streptomyces clavuligerus mtcc 1142 in solid state fermentation

A technology of solid-state fermentation and clavulanic acid, applied in the direction of fermentation, etc., can solve the problems of not clearly limiting the composition of the medium, not being able to understand, not evaluating clavulanic acid production, etc.

Inactive Publication Date: 2013-02-13
COUNCIL OF SCI & IND RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this study did not evaluate actual clavulanic acid production, nor did it clearly define the medium composition
It is unclear from this study how much clavulanic acid was present in the fermentation medium and how effective the clavulanic acid production method was

Method used

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  • Process for preparation of clavulanic acid employing streptomyces clavuligerus mtcc 1142 in solid state fermentation
  • Process for preparation of clavulanic acid employing streptomyces clavuligerus mtcc 1142 in solid state fermentation
  • Process for preparation of clavulanic acid employing streptomyces clavuligerus mtcc 1142 in solid state fermentation

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Embodiment Construction

[0039] It is to be understood that the disclosed embodiments are merely exemplary of the invention, which may be embodied in various forms. Therefore, the details disclosed herein are not to be interpreted as limiting, but merely as a basis for the claims and as a basis for teaching one skilled in the art how to make use of the invention.

[0040] A) The method is carried out under relatively simple production conditions, which include culturing a strain of Streptomyces clavulicularis for 5-7 days and recovering clavulanic acid or a salt thereof from the medium, followed by obtaining the free acid. The organism is preferably Streptomyces clavulicularis MTCC 1142 or a high-yielding mutant thereof, which is used in this method.

[0041] B) Cultivation of organisms takes place in solid, semi-solid or liquid media in the presence of assimilable sources of carbon, nitrogen and mineral salt supplements. Culturing can take place on aerobic solid media consisting of complex nutrients...

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Abstract

The present invention provides a fermentative production of clavulanic acid using agro-industrial residues and solid-state fermentation technique. The process involves cultivating the selected strain of Streptomyces clavuligerus MTCC 1142 aerobically under media conditions which are not strictly acidic or alkaline and are close to neutral, with an initial moisture content of 60-75% and uses agro-industrial residues as carbon and support. The process uses complex as well as simple nitrogen sources along with small amount of mineral salt supplementation. The process is carried out at a temperature range of 25-30 DEG C for a period extending up to 120 hrs. The process is simple to operate and offers a major advantage in the fact that it requires relatively cheaper substrates for producing clavulanic acid.

Description

technical field [0001] The present invention relates to a solid-state fermentation method for preparing clavulanic acid. More specifically, the present invention relates to the cost-effective production of clavulanic acid from agro-industrial residues under solid-state fermentation conditions. The emergence of antibiotic resistance, especially against β-lactams, has been a major target of recent attention. Drugs that used to be our first line of defense are rendered ineffective by the presence of beta-lactamases. Clavulanic acid is a novel and potent inhibitor of bacterial β-lactamases, which are produced by fermentation. Background technique [0002] Streptomyces are producers of various secondary metabolites, which are used in the preparation of pharmaceuticals. Examples of such drugs are e.g. polyketides, macrolides, anthracyclines, tetracyclines, lipopeptides and β-lactams (Strohl W.R., Biotechnology of Industrial Antibiotics, Marcel Dekker Inc., pages 1-48, 1997) . ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18
CPCC12P17/188
Inventor 保劳格·苏布哈什·绍达加尔素提尔·库马尔·辛格列哈·萨蒂什钱德拉·辛哈尔阿肖克·潘迪
Owner COUNCIL OF SCI & IND RES
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