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Biological seed-dressing agent

A seed dressing agent and biological technology, applied in the direction of biocide, biocide, animal repellent, etc., can solve the problems of environmental pollution, pesticide residues, etc., to enhance drought resistance and stress resistance, prevent seed rot, control The effect of disease occurrence

Inactive Publication Date: 2013-03-20
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional seed coating agents are composed of chemical pesticides added to film materials, which are likely to cause environmental pollution and pesticide residues after use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Add 200L of Bacillus subtilis fermentation broth (2% solid content, 10.5 billion viable bacteria / mL), 200L Bacillus licheniformis fermentation broth (2% solid content, 15.4 billion viable bacteria / mL) and 100L Streptomyces Louchei fermented broth (solid content 2%, viable count 5.3 billion / mL), stirred and mixed, added 30kg of pre-mixed adhesive according to starch: hydroxymethylcellulose ratio of 10:1. After fully stirring, add 160 kg of bentonite as a carrier medium, and continue stirring to make a homogenate.

[0016] The homogenate is transported to the LG300 spray drying tower through a peristaltic pump, spray-dried by controlling the inlet temperature at 200°C and the outlet temperature at 75°C, and the dry powder obtained is the seed dressing agent of the present invention.

[0017] Sampling was used to determine the number of viable bacteria in the seed dressing with LB bacterial culture medium plate, and the test results were as follows:

[0018] Table 1

[0...

Embodiment 2

[0021] After soaking 20g of pepper seeds in sterile water for 1 h, mix with the same amount of seed dressing agent prepared in Example 1, sow in sterile soil pre-heated (200°C, 2h), control the temperature at 25°C, Regular watering with sterile water. After the emergence of peppers, samples were taken at regular intervals to isolate endophytic Bacillus subtilis, and the number of colonies formed per unit root weight was calculated.

[0022] The result is as figure 1 As shown, the beneficial microorganism Bacillus subtilis in the seed dressing can achieve long-term colonization in the root system, and 15 days after inoculation, the number of endophytic bacteria colonization is the largest, and the colony forming unit per unit root weight is 10 6 cfu.

Embodiment 3

[0024] After soaking the pepper seeds in the spore suspension of Fusarium wilt for 1 hour, mix them with an equal amount of the seed dressing agent prepared in Example 1, and sow them in pots of high-temperature sterilized soil (200°C, 2 hours). Cultivate under the condition of ±3°C, and set the chemical fungicide carbendazim seed dressing and bentonite seed dressing as controls at the same time, and count the disease severity after 20 days.

[0025] The results showed that the biological seed dressing agent had a good control effect on watermelon Fusarium wilt, and could significantly reduce the severity of the disease, and there was no significant difference in the control effect of the commonly used chemical fungicide carbendazim. It shows that the application of biological seed dressings can not only effectively control the harm of watermelon wilt, but also help reduce the amount of chemical pesticides, reduce pesticide residues, and protect the ecological environment of fa...

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PUM

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Abstract

The invention relates to a biological seed-dressing agent, and in particular relates to a vegetable seed-dressing agent which contains soil beneficial microorganisms, relates to a method for preparing the biological seed-dressing agent, and belongs to the technical field of vegetable seed biological agents. The biological seed-dressing agent is mainly characterized by comprising bentonite which is used as a carrier medium, starch, hydroxymethyl cellulose and soil beneficial microorganism bacillus subtilis which are used as sticking agents, bacillus licheniformis and streptomyces. The biological seed-dressing agent has the effects of disease resistance, seed saving, seedling strengthening and the like as well as the advantages of no environmental pollution, safety in use, root growth and development promotion, crop drought resistance and adversity resistance enhancement, production increasing promotion and the like.

Description

technical field [0001] The invention relates to a biological seed dressing agent, in particular to a vegetable seed dressing agent containing beneficial soil microorganisms, and to a method for preparing the biological seed dressing agent, belonging to the technical field of biological preparations for vegetable seeds. Background technique [0002] my country's vegetable production is developing rapidly. The annual planting area of ​​major vegetables in the country is 280 million mu, and the vegetable output is 600 million tons, of which the area of ​​protected vegetables is 30 million mu, accounting for 80% of the world's protected gardening area. With the expansion of greenhouse vegetable area in my country and the increase of land use intensity, a series of soil quality degradation problems such as soil secondary salinization, acidification and continuous cropping obstacles have emerged. Toxic and harmful substances in the soil gradually accumulate, the number and structu...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P1/00A01P3/00A01P21/00
CPCY02A40/10
Inventor 徐健刘琴金党琴马谈斌李传明祁建杭王艳孙俊韩光杰
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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