Preparation of 7-amino-3-desacetoxy cephalosporanic acid by maize milk fermentation-enzyme catalysis method

A deacetoxycephalosporanic acid and enzyme-catalyzed technology, which is applied in the field of biological enzyme engineering, can solve the problems of the fermentation process technology not reaching industrialization, high production cost, immobilized enzymes relying on imports, and the like

Inactive Publication Date: 2008-10-15
徐州瑞赛医药科技有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Semi-synthetic and biosynthetic methods have also been introduced in China, all of which use penicillin G potassium as a raw material, and the production cost is relatively high. level of industrialization

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Cultivation of the seed medium: 1ml of the Expansionella strain of Streptomyces clavuligerus was mixed with 10 8 Conidia / ml, inoculated in the seed medium (g / l) of the following composition: Glucose 23; (NH 4 ) 2 SO 4 25; KH 2 PO 4 0.7; Lactose 50; CaCO 3 6; corn steep liquor 10; cottonseed flour 10; add water 100ml. Before disinfection, adjust the pH of the medium to 5.3. Seal the seed medium, and shake and culture it on a rotary shaker at 25°C and 220 rpm for 48 hours.

[0022] Cultivation of the fermentation medium: the cultivated seed medium is replanted in the fermentation medium with a 5% inoculum size (g / l): glucose 10; (NH 4 ) 2 SO 4 16; KH 2 PO 4 0.5; Lactose 60; CaCO 3 9; corn steep liquor 20; cottonseed meal 15; others are water. Before disinfection, adjust the pH of the medium to 6.2. After inoculation, 15% phenylacetic acid solution was added to the fermentation broth at a ratio of 1:10, cultured at 25°C and 30 rpm for 168 hours, and the evapo...

example 2

[0028] Cultivation of the seed medium: the Expansionella strain of Streptomyces clavuligerus (Streptomyces clavuligerus) with 10 9 Conidia / ml, inoculated in the following seed medium (g / l): glucose, 20; (NH 4 ) 2 SO 4 , 18; KH 2 PO 4 , 0.5; Lactose, 30; CaCO 3 , 5; corn steep liquor, 15; Pharmamedia (cotton seed flour), 12; others are water. Before sterilization, the pH of the medium was 5.3. Seal the seed medium and incubate for 42 hours at 27°C and 220 rpm.

[0029] Cultivation of the fermentation medium: the above-mentioned seed medium was inoculated into the production medium (g / l) of the following composition at a volume ratio of 1:18: glucose, 15; (NH 4 ) 2 SO 4 , 11; KH 2 PO 4 , 0.6; lactose, 23; CaCO 3 , 6; corn steep liquor, 25; Pharmamedia (cotton seed flour), 10; others are water. Before sterilization, the pH of the medium was 6.5. After inoculating with the seed medium, 15% phenylacetic acid solution 1:15 was added to the fermentation broth. Airtight...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a corn steep liquor fermentation-enzyme catalysis method, which is used to synthesize phenylacetyl-7-amino-3- desacetoxycephalos-porin acid. Streptomyces clavuligerus with an expandase gene is used to make strain transformation to corn steep liquor to ensure that the corn steep liquor is made into fermentation ring enlargement in a culture medium and is reacted with phenylacetic acid added into the culture medium to generate the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid. Water solution of the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid is obtained through organic solvent extraction and water back extraction. Acetyl transferase and nutrition content are added into the water solution to form an enzyme reaction culture medium and finish a biochemical reaction under appropriate condition, and the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid can be produced after side chains are removed.

Description

technical field [0001] The invention belongs to the technical field of biological enzyme engineering and relates to a biosynthetic method for preparing and purifying 7-amino-3-deacetoxycephalosporanic acid (7-ADCA). Background technique [0002] Cephalosporin antibiotics are an important class of β-lactam antibiotics. Originally, it was naturally produced by Acremonium chrysogenum. In recent years, it is mainly obtained through artificial synthesis. 7-Amino-3-deacetoxycephalosporanic acid (7-ADCA) is the key mother nucleus for the synthesis of cephalosporin antibiotics. 7-ADCA is prepared by artificial synthesis, and then further synthesized into cephalexin, cephradine, cephalosporin Crowe, ceftazidime and cefadroxil and other cephalosporin antibiotics. The synthesis methods of 7-ADCA mainly include chemical synthesis, semi-synthesis and biosynthesis. [0003] At present, chemical synthesis and semi-synthesis are still the main methods of synthesizing 7-ADCA: EP0169144 (...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12R1/465
Inventor 胡晓王玉成李桂根王静康王理想王友志赵红周伟军王圣良李维泉王健周平江路一飞
Owner 徐州瑞赛医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap