33 results about "Laboratory design" patented technology

The invention relates to a peanut vitamin E synthesis key gene and its application. Our laboratory designs primers based on analysis results of peanut transcriptome data, and adopts improved RACE technology to clone and isolate the cDNA sequence of peanut HPPD gene. The overexpression vector p35S::AhHPPD was constructed, and the model plant tobacco was transformed. The content of α-tocopherol in the transgenic tobacco and wild-type tobacco was analyzed by RP-HPLC. The results showed that the content of α-tocopherol in the transgenic tobacco increased by 2-3 times. It shows that AhHPPD cloned from peanut can encode functional p-hydroxyphenylpyruvate dioxygenase; the activity of AhHPPD can improve the important role of tocopherol such as α-tocopherol synthesis. This will help increase the content of vitamin E in peanuts, prolong the storage period and shelf life of oils and fats, and is of great significance.

The invention discloses a humanoid robot stable control method of an RBF-Q learning frame. The method comprises the following steps: the RBF-Q learning frame which solves the problems of state space serialization and behavior space serialization in a Q learning process is brought forward; an online motion adjusting stable control algorithm of the RBF-Q learning is brought forward, loci of the hip joint, the knee joint and the ankle joint of a support leg are generated, and a humanoid robot is controlled to walk stably through calculation of angles of other joints; and finally, the feasibility and validity of an RBF-Q learning frame method are verified on the Vitruvian Man humanoid robot platform designed by the laboratory. The method provided by the invention can generate a stable walking gait of the humanoid robot in an online learning process.

The invention provides an anti-humanized multidrug resistant protein Fab antibody and a preparation method thereof, and belongs to the field of antibodies and preparation. The preparation method comprises the following steps: performing reverse transcription of general RNA of pathological tissue of colorectal cancer in multidrug resistance to cDNA; amplifying a P-gp transmembrane domain (amino acid 784-968) gene segment by a specific primer designed in a laboratory; cloning to a pET28a(+) carrier and converting E.coliBL21(DE3), IPTG; performing inducible expression at 30 DEG C; performing WesternBolt identification for an expression product purified by a nickel ion affinity column; using purified P-gp784-968 protein as an antigen, and using 17 heavy chain upstream primers and 5 heavy chain downstream primers; for 20 light chain upstream primers and 2 light chain downstream primers, elutriating a P-gpFab antibody library of a Comb3 carrier by P-gp784-968 peptide segment in prokaryotic expression for five rounds; and finally performing double digestion identification for clones obtained by elutriation and performing IPTG (Isopropyl-Beta-d-Thiogalactoside) inductive expression; and obtaining a positive clone strain after ELISA and WesernBlot identification from the expression product, and detecting the sequence to obtain PFab29. The invention lays foundation for preparing multidrug resistant drug for inhibiting tumors.

The invention proposes a basin-type insulator life evaluation method based on artificial accelerated aging test. According to the characteristics of basin type insulators being closed, high temperature and long-term high voltage, the method uses a multi-factor laboratory to design an artificial accelerated aging test platform of electric and thermal comprehensive factors, and measures the characteristic quantities of multiple epoxy resin samples under different aging degrees. On this basis, analyze the experimental data, use the existing empirical formula, adopt the curve fitting technology and the Weibull probability distribution method, study the inner relationship between each aging factor, electrical test quantity and aging life, and will have an impact on the environment. The test data of epoxy resin material is connected with the aging evaluation of pot insulators, and a set of life evaluation methods for pot insulators is proposed.

The invention discloses a cell preparation laboratory design scheme. According to the design scheme, the laboratory comprises a preparation chamber, and a logistics chamber, a treatment chamber, a human flow chamber and a monitoring chamber are arranged sequentially around the preparation chamber; a first preparation area is arranged between the monitoring chamber and the preparation chamber; a second preparation area is arranged between the human flow chamber and the preparation chamber; a cultivation chamber and a culture medium preparation room are arranged on the side, adjacent to the treatment chamber, of the inside of the preparation chamber, the cultivation chamber is adjacent to the culture medium preparation chamber, the cultivation chamber is adjacent to the logistics chamber, and the culture medium preparation chamber is adjacent to the second preparation area; the side, adjacent to the first preparation area, of the inside of the preparation chamber is provided with a sterile operation chamber and a fourth buffering chamber, the sterile operation chamber is adjacent to the logistics chamber and the cultivation chamber, and the fourth buffering chamber is adjacent to thesterile operation chamber and the second preparation area; a washing chamber and a disinfection chamber are arranged between the fourth buffering chamber and the cultivation chamber and the culture medium preparation chamber, the washing chamber is adjacent to the disinfection chamber, the washing chamber is adjacent to the sterile operation chamber, and the disinfection chamber is adjacent to the second preparation area.