The invention discloses a detection kit for
galectin-3 and a preparation method thereof. The detection kit comprises
reagent I and
reagent II which are independent of each other.
Reagent I comprises a biobuffer, a coagulation accelerator, a
preservative, a chelating agent, an inorganic salt and water; and
reagent II comprises latex particles, the biobuffer, a surfactant, the
preservative, a suspending agent, a blocking agent and water, wherein
galectin-3 antibodies are coated by the latex particles. The latex particles with a particle size of 130 to 270nm are used for
coating galectin-3 antibodies via chemical crosslinking, so that stability, sensitivity and relatively wide detection
linear range of the detection kit are ensured. The detection kit possesses advantages such as excellent stability, high sensitivity and specificity, and convenient operation process; and is suitable for
population and application.