Colloidal gold test paper for rapidly and quantificationally detecting galectin-3

Abstract

The invention discloses a piece of colloidal gold test paper for rapidly and quantificationally detecting galectin-3. The colloidal gold test paper comprises a support gasket 5 and a nitrocellulose membrane 1 on the support gasket, wherein one end of the nitrocellulose membrane is connected with a water absorption pad 4, and the other end of the nitrocellulose membrane is connected with a conjugate gasket 2; the conjugate gasket is connected with a sample gasket 3; a first fixing part 7 is arranged under the other end of the sample gasket, and a second fixing part 6 is arranged above the other end of the sample gasket. The colloidal gold test paper is characterized in that a detection line 8 in the middle of the nitrocellulose membrane 1 is coated with a Gal-3 antibody; two control lines 9 are arranged at the two sides of the detection line, respectively; the conjugate gasket 2 is coated with a gold labeled Gal-3 monoclonal antibody or polyclonal antibody and a control line conjugate.

Description

technical field [0001] The invention belongs to the field of clinical medical diagnosis, and in particular relates to a colloidal gold test paper for quantitative detection of galectin-3 (Gal-3). Background technique [0002] Galectin-3 (Gal-3), also known as galactose-binding protein-3, has a molecular weight of 31kD and is one of the important members of the galectin family. Members of the Galectin family need to meet two criteria: first, affinity for galactose; second, significant sequence similarity in the carbohydrate recognition domain (CRD). Galectin-3 has two functional domains: the carboxy-terminal is the sugar recognition domain, which contains 135 amino acid residues; the amino-terminal contains 100-150 amino acid residues, which are connected in series by 9 amino acid residues rich in proline and glycine Repeat composition. Galectin-3 is mainly expressed in macrophages, eosinophils, neutrophils and mast cells. Its function depends on its localization in the ce...

AI Technical Summary

Problems solved by technology

[0004] At present, the method for detecting galectin-3 (Gal-3) is mainly enzyme-linked immunosorbent technique (ELISA). ELISA technology has the following disadvantages: high requirements for detection equipment, high cost; many interference factors, and poor repeatability ;Long detection time

In view of the fact that there is no rapid quantitative detection method at present, the purpose of the present invention is to provide a kind of colloidal gold test paper that can be used for rapid quantitative detection of galectin-3 and a kind of colloidal gold that can be used for rapid quantitative detection of galectin-3 Test paper box, the colloidal gold test paper and the colloidal gold test paper box have the advantages of convenience, quick operation, and accurate results.

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