33 results about "Extractive electrospray ionization" patented technology

An apparatus and method for direct analysis of continuous-flow liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS) including a sample stage that is adapted to receive a liquid sample and a nebulizing ionizer that is configured to generate a charged, nebulized solvent and thereby desorb at least a portion of the liquid sample from the sample stage.

An apparatus and method for direct analysis of continuous-flow liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS) including a sample stage that is adapted to receive a liquid sample and a nebulizing ionizer that is configured to generate a charged, nebulized solvent and thereby desorb at least a portion of the liquid sample from the sample stage.

An ion source able to ionize both liquid and gaseous vapors from interfaced liquid separation techniques and a solids / liquid atmospheric pressure (AP) probe. The liquid effluents are ionized by electrospray ionization, photoionization or atmospheric pressure chemical ionization and the vapors released from a probe device placed in a heated gas stream in the AP source are ionized by a corona or Townsend electrical discharge or photoionization. The source has the ability to ionize compounds from both liquid and solid sources, which facilitates ionization of volatile and semivolatile compounds by applying heat from a gas stream as well as highly non-volatile compounds infused by electrospray or separated by liquid chromatography or capillary electrophoresis.

Disclosed is an extractive electrospray ionization mass spectrometry method for the inside of a tissue sample. An extractive electrospray device comprises a sample bracket, a micro-syringe and a quartz capillary tube, one end of the quartz capillary tube is connected with a sampling needle of the micro-syringe, the other end of the quartz capillary tube is directly inserted into the sample to enable the tail end of the quartz capillary tube to be horizontal, but the other end of the quartz capillary tube isn't inserted through the sample, and the tail end of the quartz capillary tube is kept coaxial to the front end of the sample and an inlet of a mass spectrometer, an extracting agent is contained in the micro-syringe, a sampling pump controls flow velocity to push the extracting agent into the sample, direct current voltage is added to the sampling needle of the micro-syringe to perform extraction, desorption and ionization to the sample and form spraying at the front end of the sample, and then the sample enters the mass spectrometer to be analyzed. The extractive electrospray ionization mass spectrometry method for the inside of the tissue sample is wide in application range and capable of directly and rapidly obtaining information of molecules in the sample, and according to experiments, the method is simple in device, low in manufacturing cost, high in ionization efficiency and small in damage to the sample.

The present invention relates to an integrated system for liquid separation, such as LC, CE, affinity chromatography, and ion exchange chromatography. A preferred aspect relates to a specialised protection means for protecting the fragile electrospray needle when not in use. Another preferred aspect relates to a specialised electrical contact means for applying voltage to the electrospray needle. The invention comprises an integrated system for liquid separation and electrospray ionization comprising: a separation column; and an electrospray emitter connected with the separation column. In one aspect there is a retractable protective sleeve (8) for covering and supporting the electrospray emitter (2) along at least a portion of its axis. In another aspect there is an electrically conducting sheath surrounding the emitter and providing an electrical connection.

The invention belongs to the field of analysis and testing, and discloses a method for directly detecting chloramphenicol in honey by using ND-EESI-MS (neutral desorption-extractive electrospray ionization mass spectrometry). The method comprises the following steps: (1) preparing marked honey; (2) preparing a sample; (3) detecting by using the ND-EESI-MS; (4) selecting the secondary characteristic fragment ions m / z257 of m / z321 to perform quantitative analysis so as to obtain the amount of chloramphenicol. By using the method, the chloramphenicol in the honey can be rapidly detected and identified without pretreatment or chromatographic separation on the sample to be tested, and the defects that in the prior art, the detection steps are complex, and a long time is consumed are overcome.

The present invention relates to an integrated system for liquid separation, such as LC, CE, affinity chromatography, and ion exchange chromatography. A preferred aspect relates to a specialized protection means for protecting the fragile electrospray needle when not in use. Another preferred aspect relates to a specialized electrical contact means for applying voltage to the electrospray needle. The invention comprises an integrated system for liquid separation and electrospray ionization comprising: a separation column; and an electrospray emitter connected with the separation column. In one aspect there is a retractable protective sleeve (8) for covering and supporting the electrospray emitter (2) along at least a portion of its axis. In another aspect there is an electrically conducting sheath surrounding the emitter and providing an electrical connection.

The invention belongs to the technical field of detection and discloses a method for directly detecting chlorpyrifos in honey by using neutral desorption-extractive electrospray ionization mass spectrometry. The method comprises the following steps: (1) preparing labeled honey; (2) detecting selection of a chlorpyrifos extraction agent and a neutral desorption agent by using neutral desorption-extractive electrospray ionization mass spectrometry; and (3) selecting three-level characteristic fragment ions m / z296 of m / z352 to perform quantitative analysis, thereby obtaining the amount of chlorpyrifos in honey. According to the method disclosed by the invention, a to-be-detected sample does not need to be pretreated, the chlorpyrifos in honey is rapidly detected and identified, and the defects such as complex operating steps and high time consumption in the existing analysis technology are improved.

The invention discloses a water quality classifying method and system based on random forest. The system comprises a sampling module and a classifying module. The method comprises the following steps: acquiring water sample mass spectrum data corresponding to water quality to be tested by an extractive electrospray ionization mass spectrum technology; classifying the acquired water sample mass spectrum data through a water quality class judging model built on the basis of a random forest algorithm to obtain a classification result of the water quality to be tested. By adopting the method and the system disclosed by the invention, the water quality class judging model based on the random forest algorithm can be built easily and rapidly, and a water quality class can be identified directly without various index detection on a sample; the system has the advantages of easiness in operation, high analysis speed, high accuracy and the like. The water quality classifying method based on the random forest can be widely applied to the technical field of water quality analysis.

The invention relates to a method for quickly detecting 1-OHP (1-hydroxy pyrene) in urine by the aid of an extractive electrospray ionization tandem mass spectrum, particularly relates to a method for detecting 1-OHP in urine by the aid of a constant-pressure ionization source mass spectrum, and belongs to a means for analyzing and detecting biological markers of polycyclic aromatic hydrocarbons. The method includes operational steps: (1), preparing mother liquor of the 1-OHP; (2), preparing marking liquor of the 1-OHP; (3), preparing a marked urine sample; (4), setting parameters of an EESI (extractive electrospray ionization) source; (5), setting parameters of a tandem mass spectrum; (6), detecting the sample; and (7), processing data. The 1-OHP in the urine can be directly measured without pretreatment for the sample, and shortcomings of troublesome pretreatment process and long analysis period in the prior art are overcome. The method has the advantages of speediness, matrix interference resistance and the like, is particularly applicable to quickly screening the 1-OHP in urine of exposure population, and has an important significance in research for health risks of the PAHs (polycyclic aromatic hydrocarbons) exposure population.

The invention relates to an online-internal-extraction electrospray-ionization mass-spectroscopy detection device for rapidly analyzing body fluid samples of a human body, and belongs to the technical field of chemical analysis. The detection device consists of an eluate introduction device, an in-situ sample enriching extractor, a sample introduction device, an electrospray ionization source and a mass spectrograph, wherein the eluate introduction device is used for introducing a flowing phase for sample eluting, the in-situ sample enriching extractor is used for carrying out sample loading and online extracting and enriching, the sample introduction device is used for introducing eluted samples into the electrospray ionization source for electrospraying, and the mass spectrograph is used for carrying out detection and analysis on online samples, so as to obtain mass-spectroscopy molecular fingerprint spectrogram information on the samples. According to the detection device, the detected human body fluid samples may be human body fluids such as blood, urine, sweat and saliva, do not need sample pretreatment, and are subjected to in-situ sample loading. The device provided by the invention is simple in structure and low-cost, the practicability is high, the detection time and economic cost are reduced greatly, and a technical support is provided for the rapid diagnosis and screening of critical illnesses.

An atmospheric pressure (AP) mass spectrometry ion source able to ionize analytes in liquid effluents from interfaced liquid separation techniques and from individual containers, as in microtiter plates, by direct introduction of the analyte into the ion source using a moving ribbon, wire, or belt with vaporization of the analyte and subsequent ionization using an electric discharge or photoionization. The AP source may also incorporate atmospheric pressure chemical ionization (APCI), photoionization (APPI) and / or electrospray ionization (ESI), techniques that are commercially available. The source facilitates ionization of volatile and semivolatile compounds by applying heat from a gas stream to vaporize analyte entering the ionization region on a moving transport device. Solvent removal prior to sample entering the ionization region enables ionization of a wider range of compounds than liquid introduction APCI or APPI. The source is also capable of ionizing non-volatile compounds in solution by electrospray ionization, thus the combination provides almost universal ionization for organic compounds.

The invention discloses an adjustable device used for an extractive electrospray ionization source. The adjustable device mainly includes a universal interface device, a base, a Y-direction adjustment device, an electrospray channel nozzle parameter adjustment device, and a sample channel nozzle parameter adjustment device. The Y-direction adjustment device is capable of adjusting the heights of an electrospray channel nozzle and a sample channel nozzle. The sample channel nozzle parameter adjustment device and the electrospray channel nozzle parameter adjustment device are capable of adjusting an angle Alpha of the sample channel nozzle and a mass spectrometer sample inlet port, a distance a between the sample channel nozzle and the mass spectrometer sample inlet port, an angle Beta between the electrospray channel nozzle and the sample channel nozzle and a distance b between the electrospray channel nozzle and the sample channel nozzle. The device is applicable to researches of a relation between specific parameters and a signal intensity in a mass spectrometry technology of different forms of samples such as liquid, aerosol, solid surface and viscous samples and the like and particularly suitable for real-time online monitoring of liquid samples.

The invention discloses an MRESI (multichannel rotating electrospray ionization) mass spectrometric analysis ion source. The ion source comprises a pedestal, a stepping motor, a coupling part, a voltage conducting part, a rear support part, a rear-end bearing, a middle shaft, a front support part, a front-end bearing, a spraying needle fixed part, spraying needles and a sample platform, wherein the pedestal supports a whole set of device, and a rotor of the stepping motor and the middle shaft are coaxial; the voltage conducting part is provided with at least one conductive contact, and the spraying needles are communicated with external voltage in an electric conductance manner through wires; the front support part is sleeved on the middle shaft and the spraying needle fixed part through the front-end bearing, the rear support part is sleeved on the middle shaft through the rear-end bearing so as to support on a coaxial direction along a revolving part of the stepping motor; the left end of the middle shaft is connected with the stepping motor through the coupling part so as to transfer the rotation to connect with the spaying needle fixed part of the right end of the middle shaft; and the spraying needle fixed part is provided with multiple grooves used for installing the spraying needles, and each spraying needle is mutually communicated with an outer placing injector through an individual pipeline. The device provided by the invention has the advantages of being capable of improving ionization efficiency, enhancing stability of mass spectrometric detection signals, being used for researching gas phase among different sprayings or liquid phase reactions as well as the detection of polycomponent of complicated samples or full component.

The invention discloses a multichannel rotary extractive electrospray ionization mass spectrometry ion source. The multichannel rotary extractive electrospray ionization mass spectrometry ion source comprises an injector mounting part, at least two injector fixing parts, a spraying device mounting part, a metal ring, an insulating ring, a voltage conducting part, a front end support part, a front end connecting part, a spraying device fixing part, at least two ion source mounting parts, at least two ion source fixing parts, a spraying device, at least two spraying needles, a motor transmission part, a bearing, a motor, an injecting pump and the like. According to the ion source, the spraying needles are driven by a stepper motor to rotate to ensure that sprayed mists (electric mist or electroneutral mist) are mixed uniformly, so the ionization efficiency is improved, the strength and stability of mass spectrum signals are improved, and multicomponent or all components in a sample can be analyzed simultaneously and used for researching sample extraction or a gaseous phase / liquid phase reaction among different mists.

The invention relates to the field of analytical chemistry, and discloses an internal extraction electro-spray ionization source and a mass spectrometry method thereof. The internal extraction electro-spray ionization source includes a sampler handle 11, a quantification sampler 12, a capillary mounting fixture 13, a mounting fixture inner sleeve 14, an extractant capillary tube 15, a hand-screw joint 16, and an extractant channel 17. The sampler handle 11 and the quantification sampler 12 form a sampling device, the quantification sampler 12, the capillary mounting fixture 13, the mounting fixture inner sleeve 14, the extractant capillary tube 15, and the hand-screw joint 16 are sequentially connected and are connected with the extractant channel 17. The stability and reliability of the analysis result can be guaranteed. An electro-spray nozzle is arranged in the ionization source, so that the electrospray can be directly formed after sampling, the operation is simple, and the influence caused by differences of tissue sample lines or blood vessels can be prevented. The internal extraction electro-spray ionization source and the mass spectrometry method thereof improve the iEESI-MS stability, quantification capability, and analysis speed, and expand the iEESI-MS application range.

The invention belongs to the technical field of detection and discloses a method for directly detecting five chemical pollutants in propolis through extractive electrospray ionization mass spectrum. The method comprises the following steps: 1) preparing a standard substance solution; 2) preparing propolis tincture; 3) preparing marked propolis tincture; 4) performing extractive electrospray ionization mass spectrum detection; 5) performing qualitative analysis; and 6) performing quantitative analysis. According to the detection method provided by the invention, the dosage of the sample is small, the analysis speed is high and the high throughput and on-line detection can be realized; the common propolis chemical pollutants can be directly analyzed and detected, no complex sample pretreatment is required and the detection time for a single sample is less than 1 min. The method is non-toxic and non-pollution to products, operators and working environment. The sensitivity is high. According to the method provided by the invention, the to-be-detected sample is free from complex pretreatment and can bear the influence of complex matrix of propolis; the chemical pollutants in propolis can be directly and quickly detected; and the defects of complex sample pretreatment and long analysis time in the present analysis technique are overcome.

A device for protecting electrospray ionization (ESI) emitter tip made on a fused silica capillary nanoscale liquid chromatography (nanoLC) column for robust use of the nanoLC-ESI integrated column.

The present invention relates to electrospray ionization (ESI) at atmospheric pressure coupled with a mass spectrometer, in particular to a special kind of micro-electrospray with liquid flows in the range of 0.1 to 100 microliters per minute. The invention describes the use of an off-axis pre-entrance channel in an ESI ion source to prevent particulate matter with higher inertia than the (charged) gas molecules, such as droplets, from entering the mass spectrometer. The elimination of the particulate matter improves the quantitative precision of an LC / MS bioassay, minimizes the contamination of the mass spectrometer and improves the robustness for high throughput assays.

The invention provides a method for identifying activity of mini-sized potato seeds with EESI-MS (extractive electrospray ionization-mass spectrometry) and a multivariate statistical technique. The method comprises steps as follows: to-be-tested mini-sized potato seed samples are subjected to artificial aging acceleration processing; the mini-sized potato seeds after artificial aging acceleration treatment are subjected to a germination standard test, and germination indexes and activity indexes are calculated; the mini-sized potato seeds subjected to aging acceleration treatment are selected through random sampling for preparation of extracting solutions, and supernatants are obtained; the supernatants of the mini-sized potato seed extracting solutions are subjected to EESI-MS analysis, and the mini-sized potato seeds with different activity indexes are identified in combination with a variable analysis method. According to the method, the mini-sized potato seed extracting solutions with different activity levels are subjected to mass spectrometric analysis, and the mini-sized potato seeds with different activity indexes are identified in combination with the variable analysis method, so that the method has the advantages of being good in stability, high in sensitivity, high in analysis speed and the like.

The invention discloses an MRESI (multichannel rotating electrospray ionization) mass spectrometric analysis ion source. The ion source comprises a pedestal, a stepping motor, a coupling part, a voltage conducting part, a rear support part, a rear-end bearing, a middle shaft, a front support part, a front-end bearing, a spraying needle fixed part, spraying needles and a sample platform, wherein the pedestal supports a whole set of device, and a rotor of the stepping motor and the middle shaft are coaxial; the voltage conducting part is provided with at least one conductive contact, and the spraying needles are communicated with external voltage in an electric conductance manner through wires; the front support part is sleeved on the middle shaft and the spraying needle fixed part through the front-end bearing, the rear support part is sleeved on the middle shaft through the rear-end bearing so as to support on a coaxial direction along a revolving part of the stepping motor; the left end of the middle shaft is connected with the stepping motor through the coupling part so as to transfer the rotation to connect with the spaying needle fixed part of the right end of the middle shaft; and the spraying needle fixed part is provided with multiple grooves used for installing the spraying needles, and each spraying needle is mutually communicated with an outer placing injector through an individual pipeline. The device provided by the invention has the advantages of being capable of improving ionization efficiency, enhancing stability of mass spectrometric detection signals, being used for researching gas phase among different sprayings or liquid phase reactions as well as the detection of polycomponent of complicated samples or full component.

The invention relates to the field of analytical chemistry, and discloses an internal extraction electrospray ionization source and a mass spectrometry analysis method thereof. The internal extraction electrospray ionization source consists of a sampler handle 11, a quantitative sampler 12, a capillary holder 13, and an inner sleeve of the holder. Tube 14, extractant capillary 15, hand twist joint 16 and extractant passage 17 constitute, described sampler handle 11 and quantitative sampler 12 constitute sampling device, quantitative sampler 12, capillary tube holder 13, holder inner casing 14 , the extractant capillary 15 and the thumb joint 16 are sequentially connected and connected with the extractant passage 17. The invention guarantees the stability and reliability of the analysis results. The ionization source device has a built-in electrospray nozzle, which can be directly used to form electrospray after sampling, which is easy to operate and eliminates the influence caused by differences in texture or blood vessels of tissue samples. The invention improves the stability, quantitative ability and analysis speed of iEESI-MS, and expands the application range of iEESI-MS.

The invention relates to an electrospray extraction ionization-mass spectrum (EESI-MS) rapid detection method for alkaloid in lotus seeds, belongs to the technical field of analysis, and particularly relates to a rapid determination method for alkaloid in lotus seeds. The method comprises the following steps: processing a sample, processing a standard product, detecting by EESI-MS, drawing a standard curve, and calculating the concentration of alkaloid in a sample. The method provided by the invention is rapid and accurate without complex sample pretreatment, not only can be used for rapidly identifying the alkaloid in a complex matrix sample, but also can effectively differentiate lotus seed samples with different aging degrees by combining with chemometrics.

The present invention relates to electrospray ionization (ESI) at atmospheric pressure coupled with a mass spectrometer, in particular to a special kind of micro-electrospray with liquid flows in the range of 0.1 to 100 microliters per minute. The invention describes the use of an off-axis pre-entrance channel in an ESI ion source to prevent particulate matter with higher inertia than the (charged) gas molecules, such as droplets, from entering the mass spectrometer. The elimination of the particulate matter improves the quantitative precision of an LC / MS bioassay, minimizes the contamination of the mass spectrometer and improves the robustness for high throughput assays.

The invention relates to a universal-type ion-source nozzle of a novel structure. In the ion-source nozzle, a liquid inlet capillary tube, a gas inlet tube and a high-voltage wire are all arranged at a tail part of a basal body of the nozzle, wherein the liquid inlet caplillary tube is at the center, the gas inlet tube is on an inclined side face and an axis of the high-voltage wire is vertical to a plane in which center lines of the liquid inlet capillary tube and the gas inlet tube are located. The liquid inlet capillary tube is sealed and fixed at the tail part of the basal body of the nozzle through use of front and rear cutting sleeves and a screw head. The gas inlet tube is sealed and fixed at the tail part of the basal body of the nozzle through use of a conical screw head. The high-voltage wire is welded on a high-voltage guide head and is conductive with the basal body. The universal-type ion-source nozzle can be used as an electrospray channel nozzle and can also be used as a sample lead-in channel spray; and the universal-type ion-source nozzle is compact in structure, small in volume, mainly used to a normal-temperature and high-temperature extractive electrospray Ionization source and particularly applicable to small-scale direct mass spectrometric analysis.

The invention discloses a multichannel rotary extractive electrospray ionization mass spectrometry ion source. The multichannel rotary extractive electrospray ionization mass spectrometry ion source comprises an injector mounting part, at least two injector fixing parts, a spraying device mounting part, a metal ring, an insulating ring, a voltage conducting part, a front end support part, a front end connecting part, a spraying device fixing part, at least two ion source mounting parts, at least two ion source fixing parts, a spraying device, at least two spraying needles, a motor transmission part, a bearing, a motor, an injecting pump and the like. According to the ion source, the spraying needles are driven by a stepper motor to rotate to ensure that sprayed mists (electric mist or electroneutral mist) are mixed uniformly, so the ionization efficiency is improved, the strength and stability of mass spectrum signals are improved, and multicomponent or all components in a sample can be analyzed simultaneously and used for researching sample extraction or a gaseous phase / liquid phase reaction among different mists.

The invention belongs to the field of analysis and testing, and discloses a method for directly detecting chloramphenicol in honey by using ND-EESI-MS (neutral desorption-extractive electrospray ionization mass spectrometry). The method comprises the following steps: (1) preparing marked honey; (2) preparing a sample; (3) detecting by using the ND-EESI-MS; (4) selecting the secondary characteristic fragment ions m / z257 of m / z321 to perform quantitative analysis so as to obtain the amount of chloramphenicol. By using the method, the chloramphenicol in the honey can be rapidly detected and identified without pretreatment or chromatographic separation on the sample to be tested, and the defects that in the prior art, the detection steps are complex, and a long time is consumed are overcome.