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Method for quickly detecting 1-OHP (1-hydroxy pyrene) in urine by aid of extractive electrospray ionization tandem mass spectrum

A 1-OHP and tandem mass spectrometry technology, which is applied in the field of rapid detection of 1-OHP in urine and polycyclic aromatic hydrocarbon biomarkers by electrospray extraction ionization tandem mass spectrometry, can solve the problems that cannot meet urgent needs and take a long time , to improve selectivity and accuracy, reduce matrix interference, and shorten the analysis cycle

Inactive Publication Date: 2012-10-10
SHANGHAI UNIV +1
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Problems solved by technology

[0005] However, the existing methods for the analysis of 1-OHP in urine generally take a long time for sample pretreatment and chromatography, which cannot meet the urgent needs of high-throughput sample analysis in PAHs health risk assessment (such as PAHs molecular epidemiology)

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  • Method for quickly detecting 1-OHP (1-hydroxy pyrene) in urine by aid of extractive electrospray ionization tandem mass spectrum
  • Method for quickly detecting 1-OHP (1-hydroxy pyrene) in urine by aid of extractive electrospray ionization tandem mass spectrum
  • Method for quickly detecting 1-OHP (1-hydroxy pyrene) in urine by aid of extractive electrospray ionization tandem mass spectrum

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Embodiment Construction

[0029] Preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. The present invention is not limited to the following embodiments. The experimental conditions in the embodiments are only used as illustrations of typical situations, and are not limitations of the present invention.

[0030] The ionization source used in this embodiment is the electrospray extraction ionization source manufactured by Jiangxi Provincial Key Laboratory of Mass Spectrometry Science and Instruments, East China University of Science and Technology. Xcalibur data processing system of American Finnigan Company.

[0031] The method for rapid detection of 1-OHP in urine by electrospray extraction ionization tandem mass spectrometry, the specific operation steps are as follows:

[0032] (1) Preparation of 1-OHP mother solution: Accurately weigh 10.0 ± 0.5 mg 1-OHP, put it into a 100-mL volumetric flask, add methanol to dissolve, and ob...

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Abstract

The invention relates to a method for quickly detecting 1-OHP (1-hydroxy pyrene) in urine by the aid of an extractive electrospray ionization tandem mass spectrum, particularly relates to a method for detecting 1-OHP in urine by the aid of a constant-pressure ionization source mass spectrum, and belongs to a means for analyzing and detecting biological markers of polycyclic aromatic hydrocarbons. The method includes operational steps: (1), preparing mother liquor of the 1-OHP; (2), preparing marking liquor of the 1-OHP; (3), preparing a marked urine sample; (4), setting parameters of an EESI (extractive electrospray ionization) source; (5), setting parameters of a tandem mass spectrum; (6), detecting the sample; and (7), processing data. The 1-OHP in the urine can be directly measured without pretreatment for the sample, and shortcomings of troublesome pretreatment process and long analysis period in the prior art are overcome. The method has the advantages of speediness, matrix interference resistance and the like, is particularly applicable to quickly screening the 1-OHP in urine of exposure population, and has an important significance in research for health risks of the PAHs (polycyclic aromatic hydrocarbons) exposure population.

Description

technical field [0001] The invention relates to a detection method of 1-hydroxypyrene (1-OHP) in urine, in particular to a method for rapid detection of 1-OHP in urine by electrospray extraction ionization tandem mass spectrometry, which belongs to the analysis and detection means of polycyclic aromatic hydrocarbon biomarkers. Background technique [0002] 1-OHP in urine is an important biomarker of carcinogenic environmental pollutants polycyclic aromatic hydrocarbons (PAHs), which is of great significance in human exposure to PAHs and health risk assessment studies (such as PAHs molecular epidemiology). [0003] The analysis methods of 1-OHP in urine reported in the literature include: high performance liquid chromatography fluorescence detection (HPLC-FD), RTF, SFS, resonance light scattering (resonance light scattering spectroscopy, RLS) , gas chromatography (hydrogen flame ionization detector) (gas chromatography flame ionization detection, GC-FID), gas chromatography m...

Claims

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Application Information

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IPC IPC(8): G01N27/62
Inventor 李雪陈焕文李银萍方小伟于志强傅家谟
Owner SHANGHAI UNIV
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