35 results about "Cattleya" patented technology

The invention discloses a breeding method for vanda. The breeding method comprises the following steps of: selecting a full terminal bud which is germinated in the current year as a material; removing redundant leaves for induction culture; germinating strong buds after 15-20 days; carrying out enrichment culture on the buds for 20-30 days; and performing root induction on the buds for 20-25 days to obtain seedlings for transplanting, wherein the survival rate is higher than 95%. The expanding propagation coefficient is high, and nursery-grown plants grow well after surviving. The vanda is single-axis stem cattleya, so that lateral buds are almost not generated. Moreover, stem tips contain many polyphenols with strong activity, and the cut stem tips are easy to brown during the culture, so that the culture is difficult to succeed; and natural sexual propagation is difficult to succeed as seed germination depends on symbiotic bacteria nourishing. The technical scheme provided by the invention solves the problems of low survival rate, slow propagation and the like in the prior art, and has remarkable economic and social benefits.

The invention relates to the technical field of plant tissue culture, in particular to a Cattleya tissue culture propagation method, comprising the following steps: material selection, explant pretreatment, explant disinfection, induction culture, proliferation culture, strong seedling rooting and transplanting , Cattleya can be rapidly cultivated and propagated through the present invention, and the cultivated Cattleya seedlings have a high seedling rate, good quality, and strong adaptability after cultivation, and meet the requirements of commercialization in a short period of time by means of biotechnology. The demand for production provides technical support for the large-scale and industrialized cultivation of Cattelan. At the same time, the tissue culture propagation method has good disinfection effect and low browning rate, thereby greatly improving the seedling rate and work efficiency, and saving resources.

The invention discloses Cattleya pollen germination medium, which is liquid medium comprising cane sugar, H3BO3 and CaCl2. Preferably, concentrations of the cane sugar, the H3BO3 and the CaCl2 are respectively 100g / L-1, 40mg / L-1 and 150mg / L-1. By using the Cattleya pollen germination medium, germination of pollen can be observed after 3 hours, pollen mass completely dissolves in the liquid after 24 hours, germination of the pollen can be observed vividly, and efficiency can be improved greatly.

The present invention disclose a regulating cattleya bloom during the new year's day and the spring festival, proceeding low-temperature control in the bud sheath-breaking phase of the cattleya, the daytime average temperature is kept at 10 DEG, and 6 DEG at night, which can delay the florescence of the cattleya; proceeding heating treatment one week before the new year's day or the spring festival, the day temperature is kept at 23 DEG and the 20 DEG at night to enable the cattleya bloom during the new year's day and the spring festival without any influence to the flowering quality, and the flowering regularity is more than 95%, so that increasing commodity value of the cattleya and implementing commercial production of the cattleya.

The invention discloses a method for screening tulasnella fungi and establishing a symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida. A Tcs1 bacterial strain isadopted and establishes a symbiotic relation together with one or more orchid seeds including cymbidium mannii seeds, cattleya hybrid YK seeds and cattleya hybrid YD seeds to promote germination of orchid seeds. A symbiotic germination test is carried out on an artificial medium through adoption of tulasnella fungi and orchid seeds, and is compared with a control group of orchid seeds with no fungi inoculated to screen mycorrhizal fungi capable of widely efficiently promoting seed germination and to screen appropriate symbiotic mediums. Efficient symbiotic fungi for a germination stage of orchid seeds are obtained, the germination rate of orchid seeds is improved via symbiotic germination culture technique, and a symbiotic system of orchid and mycorrhizal fungi is established.

The invention provides the rapid propagation technology of Cattleya seedlings. Cattleya is known as the king of orchids, with beautiful flower shapes, bright colors and high ornamental value. The trade volume in the international market is huge, and it may also become a new tropical orchid sales hotspot after Phalaenopsis and Whelan orchid in the Chinese market. However, the existing conventional production technology adopts solid medium for seeding, which takes a long time to germinate and grows slowly; the operation is labor-intensive, the cultivation time is long, and the cost is high. The purpose of the present invention is to improve the production process and use liquid culture in the early stage to promote the rapid germination and growth of seeds, and then directly inoculate the protocorms on the seedling medium to form seedlings in one step, reducing intermediate links, greatly reducing costs and cultivation time, The technology can be applied to the seedling production and breeding of hybrids within the genus Cattleya and close genera, and has a good application prospect.

The invention discloses a cattleya florescence regulating and controlling method. The selected cattleya plants are put into a greenhouse with a day temperature of 25 DEG C and a night temperature of 20 DEG C, photoperiod is set with a light period of 12h and a night period of 12h, a light intensity of 320[mu]mol.m<-2>.s<-1> and an air humidity of 70%-80%, and the process continues for six weeks. High P fertilizer with a N:P:K ratio of 10:30:20 is applied for forcing the cattleya, and the plants are sprayed once each week by monopotassium phosphate aqueous solution diluted by 1000 times for improving cold resisting ability and in-vivo nutrient accumulation of the plants, and further improving the flowering. From September, the high P fertilizer is applied for forcing the cattleya, the cattleya is fertilized once after watering until the florescence is stopped. Therefore, the cattleya is abloom in time before holidays such as National Day, Spring Festival and the like, and the commodityvalue of the cattleya can be increased.

The invention discloses a method for realizing in-vitro preservation of cattleya germplasm resources and recovering the growth after the preservation. The method comprises the following steps: stripping a stem tip from a new growing pseudobulb, putting the stem tip on a surface of a protocorm induction culture medium and culturing to obtain a protocorm; carrying out repeated cutting and subculture on the protocorm, so as to reproduce the protocorms with the needed quantity; culturing by adopting an in-vitro preservation culture medium; after the protocorms enter a development and growth phase, culturing in a dark place at 0 to 5 DEG C; transferring the protocorms which are cultured in the dark place into a differentiation culture medium; differentiating the protocorms to generate seedlings. According to the method disclosed by the invention, a cattleya stem tip culture technology is utilized to induce the protocorms and the protocorms are differentiated to obtain the cattleya seedlings. The protocorms are preserved at low temperature and a proper amount of paclobutrazol is added, so that the preservation time can be greatly prolonged and the number of times of subculture is reduced; the hereditary properties of the germplasm resources are effectively kept. After the protocorms are preserved for certain time, the protocorms can be cultured at normal temprature and under illumination conditions, and the growth of the protocorms can be rapidly recovered and the seedlings are differentiated.

The invention discloses a breeding method for vanda. The breeding method comprises the following steps of: selecting a full terminal bud which is germinated in the current year as a material; removing redundant leaves for induction culture; germinating strong buds after 15-20 days; carrying out enrichment culture on the buds for 20-30 days; and performing root induction on the buds for 20-25 days to obtain seedlings for transplanting, wherein the survival rate is higher than 95%. The expanding propagation coefficient is high, and nursery-grown plants grow well after surviving. The vanda is single-axis stem cattleya, so that lateral buds are almost not generated. Moreover, stem tips contain many polyphenols with strong activity, and the cut stem tips are easy to brown during the culture, so that the culture is difficult to succeed; and natural sexual propagation is difficult to succeed as seed germination depends on symbiotic bacteria nourishing. The technical scheme provided by the invention solves the problems of low survival rate, slow propagation and the like in the prior art, and has remarkable economic and social benefits.

The invention discloses a primary cattleya hybrida inducing medium and an explant pretreatment method. The primary cattleya hybrida inducing medium takes an improved MS culture medium as a basic culture medium; citric acid, salicylic acid, lanthanum nitrate, 6-BA, NAA, agar powder, cane sugar and silica gel are added into the basic culture medium; the browning of the cattleya hybrida explants can be effectively alleviated.

The present invention relates to the preparation of plant cell cultures belonging to the Dendrobium Phalaenopsis, Ansellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera and their use in cosmetic, nutritional and pharmaceutical fields. In particular, the present invention regards a selected cell culture of meristematic cells of plants belonging to the Dendrobium Phalaenopsis, Ansellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera characterized in that it contains an amount of phenylpropanoids greater than 0.1% expressed on the cell dry weight and an amount of hydrosoluble polysaccharides greater than 5% expressed on the cell dry weight, as well as preparations, pharmaceutical and cosmetic compositions or food supplements containing the cell culture.

The invention provides a Cattleya SSR molecular marker primer composition, a fingerprint code and its application in the identification of Cattleya germplasm resources, belonging to the technical field of molecular biology. The present invention provides 15 SSR molecular markers of Cattleya and their amplification primer compositions, and utilizes TP-M13-SSR technology to analyze the genetic diversity of 26 different germplasm resources within the genus and construct molecular ID cards , can accurately, efficiently and stably identify the germplasm resources of Cattleya, and lay a solid foundation for the identification of germplasm resources of Cattleya, analysis of kinship, mapping of trait genes, molecular marker-assisted breeding and related molecular research of Cattleya. Base.

The invention provides a Chinese mugwort essence oil perineum stick and a production process and application thereof. The Chinese mugwort essence oil perineum stick comprises an inner Chinese mugwort essence oil herb layer and an outer breathable cotton layer, a stickup layer is arranged on the bottom layer of the breathable cotton layer, and the Chinese mugwort essence oil herb layer mainly comprises, by weight, 3-5 parts of Chinese mugwort essence oil, 15-20 parts of mulberry twigs, 2-5 parts of rhizome of rehmannia, 1-2 parts of rheum officinale, 2-6 parts of cattleya, 1-2 parts of mastic, 2-4 parts of radix angelicae tuhuo, 1-3 parts of cortex phellodendri, 1-3 parts of achyranthis bidentatae, 5-8 parts of eucommia ulmoides, 5-7 parts of mistletoe, 6-9 parts of rhizoma curculiginis, 10-13 parts of herba epimedii and 25-35 parts of folium artemisiae argyi. The Chinese mugwort essence oil perineum stick is convenient to use and carry, simple in operation, safe and comfort and more remarkable in effect after multiple use and has efficacies of nourishing yin and tonifying yang, inhibiting bacterial and protecting the uterus and detoxifying and beautifying.

The invention discloses R2R3MYB transcription factors that promote anthocyanin formation in two kinds of orchids. The transcription factors are shown in SEQ ID NO: 1 and SEQ ID NO: 2, and the transcription factors are isolated and identified from Cattleya. The corresponding transcription factors can enrich the transgenic material library for the red trait of orchid flowers, which provides strong support for the molecular breeding of orchid red trait flowers in the future.