Asepsis sowing and tissue culturing technique of Cattleya bowringiana Hort.

Abstract

The invention provides a rapid breeding method of Cattleya by improving the current flow of production, the method comprises fluid culturing on earlier stage, so as to promote the seed to grow fast, then directly inoculating the original bulbodium to the sprout culture medium for sprout production in one step. The method can reduce culture cost and time greatly, and can be applied to Catteva genus, near source bigeneric hybid seedling production and breeding works.

Description

technical field [0001] The invention provides the rapid propagation technology of Cattleya seedlings. Background technique [0002] Cattleya is known as the king of orchids. There are large flower strains and miniature strains, with beautiful flower shapes, bright colors and high ornamental value. It can be used for potted plants or hanging pots, and is also widely used for cut flowers. The trade volume in the international market is huge, and it may also become a new tropical orchid sales hot spot in the Chinese market after Phalaenopsis and Cymbidium phalaenopsis. [0003] Most of the Cattleya commercial seedlings are seedlings, and the current production process is as follows: [0004] Seed germination medium (mother bottle) → growth and proliferation medium (mother bottle) → seedling growth medium [0005] It takes 2 months to half a year from seed germination to subculture on solid germination medium depending on the variety. After the seedlings are formed in the moth...

AI Technical Summary

Problems solved by technology

[0005] It takes 2 months to half a year from seed germination to subculture on solid germination medium depending on the variety. After the seedlings are formed in the mother bottle, they are then transferred to the seedling medium for strong seedlings to take root. Need to spend a lot of labor and training costs

[0006] Cattleya can be propagated by division, but the propagation speed is slow, and Cattleya seeds are extremely difficult to germinate under natural conditions due to incomplete embryo development.

A large amount of test-tube seedlings can be obtained by using the method of test-tube aseptic sowing, but the existing conventional production technology uses solid medium for sowing, and the germination time of the seeds is long and the growth is slow; For seedling cultivation, after the seeds germinate, they need to go through the mother bottle stage, which is labor-intensive to operate, takes a long time to cultivate, and costs high