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Aseptic seeding medium for broad-spectrum tropical orchids

A technology for aseptic sowing and culture medium, which can be used in culture medium, soilless culture, planting substrate, etc., and can solve the problems of poor broad-spectrum and germination rate of seeds failing to germinate.

Inactive Publication Date: 2017-06-09
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently disclosed medium has the defect of poor broad-spectrum. Generally, each tropical orchid corresponds to a kind of sowing medium, and the seeds of other types of orchid sowing medium cannot germinate or have a low germination rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0010] Broad-spectrum Tropical Orchid Aseptic Sowing Medium of the present invention, its preferred embodiment is:

[0011] Each L medium includes:

[0012] Huabao 1# 2g, tryptone 1g, banana 20g, mashed potato 100g, sucrose 10g, activated carbon 2g, agar 5.8g, pH=5.7.

[0013] Cultivate at the stage of mature fruit pods: take uncracked fruit pods and take them when the stalks of the fruit pods start to turn yellow.

[0014] The culture temperature is 24 to 26 ° C, and the light intensity is 60 μmol m -2 the s -1 , light time 12 hours / day.

[0015] The embodiments of the present invention will be further described in detail below.

[0016] Harvesting period of mature fruit pods: when the fruit pods are not cracked and the stalks of the fruit pods start to turn yellow.

[0017] The culture medium is: Huabao 1# 2g / L+tryptone 1g / L+banana 20g / L+mashed potato 100g / L+sucrose 10g / L+activated carbon 2g / L+agar 5.8g / L, pH=5.7.

[0018] The culture temperature is about 25℃, and th...

specific Embodiment

[0020] (1) The fruit pods used for aseptic sowing are better when they are just mature but not yet cracked. Generally, it is better when the stalk of the fruit pods starts to turn yellow. At this time, the seeds in the capsule can be separated from each other and are easy to sow. Immature seeds stick together when sown, which is not conducive to later growth. In addition, uncracked fruit pods are easy to sterilize, and only the pod skin can be sterilized; after the fruit pods are cracked, it is more difficult to sterilize the powdered seeds, and the germination ability of the seeds is reduced. It is advisable to pick and sow the fruit pods immediately after harvesting. When it is time to sow in the future, they can be stored in a sealed plastic bag and stored in a refrigerator at 4°C, but the time should not be too long, otherwise it is easy to grow mold or reduce the germination rate.

[0021] (2) Sterile seeding must be carried out on the ultra-clean workbench. Prepare all ...

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Abstract

The invention discloses an aseptic seeding medium for broad-spectrum tropical orchids; each L of the medium comprises 2 g of Hyponex No. 1, 1 g of tryptone, 20 g of banana, 100 g of mashed potato, 10 g of sucrose, 2 g of activated carbon, and 5.8 pg of agar, under pH of 5.7. Culturing is performed in the stage of mature capsules; to be specific, non-cracked capsules are picked from the time when stems of the capsules start to yellow. Culturing temperature is 24-26 DEG C, light intensity is 60 Mumol.m<-2>s<-1>, and illumination time is 12 h / d. After the use of the medium, germination rate of Paphiopedilum seeds is 70% and above, germination rate of Cattleya, Phalaenopsis and Dendrobium seeds is 90% and above, and germination rate of seeds of other tropical orchid is 80% and above.

Description

technical field [0001] The invention relates to a culture medium for flowers, in particular to a broad-spectrum tropical orchid aseptic sowing medium. Background technique [0002] Orchid is one of my country's top ten famous flowers and one of the world's famous and precious flowers. It occupies a very important position in flower production. With the rapid development of the economy, the acceleration of urbanization and the improvement of people's living standards, traditional orchids can no longer meet people's growing aesthetic needs. It has become the darling of flowers in the market because of its extraordinary beauty, changeable flower shape, long flowering period and rich and colorful colors. [0003] A large number of seedlings can be obtained by aseptic sowing, which is the main means of cross breeding to cultivate new varieties. However, the currently disclosed medium has the defect of poor broad-spectrum. Generally, each tropical orchid corresponds to a seeding...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01G31/00
CPCA01G24/00C05C11/00C05G3/00C05D9/00C05F11/00C05G1/00
Inventor 王雁郑宝强
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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