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Method for tissue culture and rapid propagation of Dendrobium nobile variety 'Huoniao'

A technology for tissue culture rapid propagation and dendrobium spring, applied in the field of agricultural biology, can solve the problem of no 'Firebird' tissue culture rapid propagation, etc., and achieves the effect of speeding up reproduction and speeding up the base number of seedlings

Active Publication Date: 2016-11-09
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Dendrobium stardust ‘Fire Bird’ is a species of Dendrobium orchidaceae, and there is no report on tissue culture and rapid propagation of Dendrobium stardust ‘Fire Bird’ in the literature

Method used

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  • Method for tissue culture and rapid propagation of Dendrobium nobile variety 'Huoniao'
  • Method for tissue culture and rapid propagation of Dendrobium nobile variety 'Huoniao'
  • Method for tissue culture and rapid propagation of Dendrobium nobile variety 'Huoniao'

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: Screening spring Dendrobium species 'Firebird' induction medium with the method of the present invention

[0028] 1) Selection of explants: Select the spring Dendrobium species 'Firebird', which grows robustly and has no diseases and insect pests, as explants, cut into 3 cm stem segments, leave 1 to 2 buds for each stem segment, and wash with detergent Wash for 10 minutes, then rinse with tap water for 3 hours, and finally dry the surface of the material for later use;

[0029] 2) Tender stem induction culture: Soak the stem section of the spring Dendrobium species 'Firebird' with a mass concentration of 75% alcohol for 30 seconds on an ultra-clean workbench, then disinfect it with 0.3% mercury liter for 7 minutes, and finally Rinse 3 times with sterile water, then place the tender stems on sterile filter paper to absorb excess water on the surface, inoculate them on the stem segment induction medium, and place the inoculated explants at a temperature of 2...

Embodiment 2

[0030] Embodiment 2: Carry out spring dendrobium kind ' Huo Niao ' bushy bud proliferation subculture with the method of the present invention

[0031] Put the clustered buds into the culture medium for the proliferation of clustered buds, place them in a culture room with a temperature of 25±2°C, a light of 12h / d, and a light intensity of 2000-3000lx, and subculture once every 30-40d, and subculture 3 times to generate growth Robust tissue-cultured seedlings (the multiplication coefficient of clustered buds reaches an average of 5.3 at the third subculture);

[0032] 3) rooting of strong seedlings: adopt 2 / 3DKW medium to carry out rooting culture for robust test-tube seedlings, and grow into rooted spring dendrobium variety 'Firebird' seedlings after 30 days;

[0033] 4) hardening and transplanting: the rooted Dendrobium species 'Firebird' seedlings are hardened and transplanted to form the spring Dendrobium variety 'Firebird' tissue culture seedlings.

[0034] The method of...

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Abstract

The invention belongs to the field of plant tissue culture, and discloses a method for tissue culture and rapid propagation of Dendrobium nobile variety 'Huoniao'. The method mainly comprises the following steps: 1, disinfecting current-year-grown stems in spring, used as an explant, in NaClO and HgCl2; 2, grafting the disinfected explant to an induction medium MS + 6-BA 3 mg.L<-1> + NAA 0.2 mg.L<-1>, and culturing the explant; 3, transferring the explant to a multiplying medium MS +TDZ 0.1 mg.L<-1> + NAA 0.2 mg.L<-1> + 200 g.L<-1> mashed potato + 1 g.L<-1> active carbon, and culturing the explant; 4, transferring the explant to a seedling strengthening and rooting medium 1 / 2MS + 0.5 mg.L<-1> IBA + 200 g.L<-1> mashed potato, and culturing the explant; and 5, hardening seedlings, and transplanting the seedlings to obtain Dendrobium nobile variety 'Huoniao' tissue culture seedlings. The method for tissue culture and rapid propagation of 'Huoniao' realizes propagation coefficient maximization in a shortest time, and is in favor of rapidly growing seedlings of the cattleya dendrobium variety 'Huoniao', shortening the seedling stage and the growing period in order to reach the purposes of rapid propagation speed, production cost saving and suitableness for large-scale seedling growth. The method has the advantages of extremely small amount of a used plant material, fast growth, short period, strong repeatability, and realization of annual test or production.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and relates to a tissue culture and rapid propagation method of the spring dendrobium variety 'Huo Niao'. Background technique [0002] Dendrobium stardust ‘Fire Bird’ is a species of Dendrobium orchidaceae, and there is no report on tissue culture and rapid propagation of Dendrobium stardust ‘Fire Bird’ in the literature. This method provides a new method for the rapid propagation of the spring dendrobium variety 'Firebird', and provides a new breeding method for the industrialization of the spring dendrobium variety 'Firebird' as spring dendrobium. Contents of the invention [0003] In order to change the poor breeding and cultivation status of the current Dendrobium species 'Firebird', the purpose of the present invention is to accelerate the cultivation and development of the Dendrobium species 'Firebird', improve the reproductive efficiency of the Dendrobium species 'Firebird', an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李振坚孙振元张俊民杨业波刘俊祥彭向永龚晓慧
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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