78 results about "Acute lymphoblastic leukemias" patented technology

The invention relates to the technical field of medical detection, in particular to a detection kit for minute residues of B-cell acute lymphocyte leukemia. The detection kit comprises anti CD10, CD19, CD20, CD34, CD38 and CD45 antibodies labeled by different fluoresceins. The detection kit comprises six monoclonal antibody combinations of leukocyte differentiation antigen, and a fluorescence labeling and flow cytometric detection technology is combined to realize that one-time flow cytometric detection can quickly and accurately identify the level and typing of abnormal cells of the minute residues of the B-cell acute lymphocyte leukemia, the obtained detection index can be used as an intermediate result to intuitively judge the prognosis conditions of B-cell acute lymphocyte leukemia patients, and thus important guiding significance is provided for the formulation of clinical treatment schemes for the leukemia patients.

The invention relates to tubulin depolymerizing agent polypeptides and application thereof, particularly polypeptides capable of inhibiting polymerization of tubulin and treating acute lymphatic leukemia. The invention is characterized in that the sequence of the tubulin depolymerizing agent polypeptides 1 is CRALERLV disclosed as SEQ NO.1. The invention also relates to application of the tubulin depolymerizing agent polypeptides 1 in preparation of medicines for treating acute lymphatic leukemia. A solid-phase synthesis method is utilized to chemically synthesize the tubulin depolymerizing agent polypeptides 1; and the polypeptides with the bran-new sequence can inhibit tubulin in vitro and treat acute lymphatic leukemia.

The invention discloses a kit for detecting gene mutation of acute lymphoblastic leukemia based on multiplex-PCR targeted high-throughput sequencing and a detection method of the kit. The kit is usedfor detecting multiple exon regions of 19 genes related with the acute lymphoblastic leukemia. The kit contains a primer working solution for detecting relevant gene mutation, a PCR mixed solution, aPCR reaction solution, a digestion solution, a joint P5 and a joint P7. The kit is used for carrying out detection based on a multiplex-PCR targeted high-throughput sequencing technique, has the advantages of high accuracy, sensitivity and throughput and can be used for rapidly and efficiently carrying out standardized quantitative detection on 19 relevant genes of acute lymphoblastic leukemia (ALL).

The invention provides a mouse model of acute lymphoblastic leukemia and a modeling method, and relates to the field of biotechnology. The preparation method comprises the following steps: infecting IL-7 stimulated hematopoietic cells with retroviruses with BCR-ABLP190; and transfusing the stimulated hematopoietic cells to syngenic mice. The method has the advantages of being simple and easy to implement in a modeling process and capable of establishing the ph+ALL mouse model in one step. According to the mouse model of the acute lymphoblastic leukemia provided by the invention, the sorted BCR-ABL+B cells are transfused to the syngenic mice for the second time without irradiation treatment, with performance consistent with that of mice of first episode; the model is good in stability; and in addition, the uniformity of recipient mice is enhanced and such risks as accidental death of the recipient mice due to irradiation are reduced.

The invention relates to the field of diagnosis kits, and particularly relates to a pair of primer and kit for detecting the specific gene HB-1 of acute B lymphocyte leukemia. The primers for detecting the HB-1 and a probe are an upstream primer sequence as shown in SEQ ID NO:1, a downstream primer sequence as shown in SEQ ID NO:2 and a probe as shown in SEQ ID NO:3; the 5'end of a nucleotide sequence as shown in the SEQ ID NO:3 has a Fam mark, and the 3'end of the nucleotide sequence has a Tamra mark. The kit disclosed by the invention optimizes the probe sequence, simplifies the experimental step, realizes the single step of a detection method, enhances the experimental stability and realizes the quantitative detection of the gene HB-1.

The invention discloses application of fatsia japonica branch and leaf extractives to anti-cancer drug preparation and preparation of the fatsia japonica branch and leaf extractives, and belongs to the technical field of medicines and natural medicines. A preparation method of the fatsia japonica branch and leaf extractives includes the following steps: ethyl alcohol with 95-percent concentration serves as extracting agents; an ultrasonic extraction method is adopted for extracting fatsia japonica branches and leaves; total ethyl alcohol extractives of the fatsia japonica branch and leaf extractives are obtained; petroleum ether, chloroform and ethyl acetate are sequentially used for extracting the total ethyl alcohol extractives; a petroleum ether part, a chloroform part, an ethyl acetate part and an extraction remaining part are obtained. Meanwhile, supersonic extraction is carried out on the fatsia japonica branches and leaves obtained after extraction with the ethyl alcohol is carried out with distilled water, and an aqueous extract part is obtained. The five extraction parts of the fatsia japonica branches and leaves are of great significance in preparing medicines for treating the breast cancer, the non-small cell lung cancer and the human B-cell acute lymphoblastic leukemia.

The invention relates to succinyl hydrazine compounds derivative and medical compound containing them; and the application of it in preparing medicine that resists leukemia. The succinyl hydrazine compounds derivative shows inhibiting action to BCR-Ab1 tyrosine kinase activity through pharmacological test. It is characterized by high effecivity and low toxicity, and can be widly used to prepare medicine that treats chronic granulocytic leukemia (CML) and philadelphia chromosome positive acute lymphoblastic leukemia, especially medicine treating patient that generates drug resistance to Gleevec.

The present invention discloses a kit for simultaneously detecting mutations of genes NBPF10, TSFM, PRB2 and DIAPH1, wherein the kit comprises a plurality of multi-gene capture probes, can be used fordetecting the somatic mutations and the germline mutations at the tumor genome level, can be used for the assisted diagnosis of patients with hematological tumors (especially acute lymphoblastic leukemia patients), the monitoring of minimal residual diseases, the evaluation of prognosis, and the like, and can provide important effect in the field of medical detection, wherein the detection methodis simple and sensitive.